The TCP transcription factor PeTCP10 modulates salt tolerance in transgenic Arabidopsis

Xu, Yuzeng; Liu, Huanlong; Gao, Yameng; Xiong, Rui; Wu, Min; Zhang, Kaimei; Xiang, Yan

doi:10.1007/s00299-021-02765-7

Cited by 41 publications

(21 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also enhances the expression levels of stress- and ROS-scavenging-associated genes in the cold stressed A. thaliana plants ( Cheng et al, 2019 ). Overexpressed PeTCP10 gene improved salt and ABA tolerance of transgenic A. thaliana at the vegetative growth phase ( Xu et al, 2021 ). These results indicate that TCP genes pointedly contribute to hormone signaling pathways and abiotic stress tolerance in various plant species.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive In Silico Characterization and Expression Profiling of TCP Gene Family in Rapeseed

et al. 2021

View full text Add to dashboard Cite

TCP proteins are plant-specific transcription factors that have multipurpose roles in plant developmental procedures and stress responses. Therefore, a genome-wide analysis was performed to categorize the TCP genes in the rapeseed genome. In this study, a total of 80 BnTCP genes were identified in the rapeseed genome and grouped into two main classes (PCF and CYC/TB1) according to phylogenetic analysis. The universal evolutionary analysis uncovered that BnTCP genes had experienced segmental duplications and positive selection pressure. Gene structure and conserved motif examination presented that Class I and Class II have diverse intron-exon patterns and motifs numbers. Overall, nine conserved motifs were identified and varied from 2 to 7 in all TCP genes; and some of them were gene-specific. Mainly, Class II (PCF and CYC/TB1) possessed diverse structures compared to Class I. We identified four hormone- and four stress-related responsive cis-elements in the promoter regions. Moreover, 32 bna-miRNAs from 14 families were found to be targeting 21 BnTCPs genes. Gene ontology enrichment analysis presented that the BnTCP genes were primarily related to RNA/DNA binding, metabolic processes, transcriptional regulatory activities, etc. Transcriptome-based tissue-specific expression analysis showed that only a few genes (mainly BnTCP9, BnTCP22, BnTCP25, BnTCP48, BnTCP52, BnTCP60, BnTCP66, and BnTCP74) presented higher expression in root, stem, leaf, flower, seeds, and silique among all tested tissues. Likewise, qRT-PCR-based expression analysis exhibited that BnTCP36, BnTCP39, BnTCP53, BnTCP59, and BnTCP60 showed higher expression at certain time points under various hormones and abiotic stress conditions but not by drought and MeJA. Our results opened the new groundwork for future understanding of the intricate mechanisms of BnTCP in various developmental processes and abiotic stress signaling pathways in rapeseed.

show abstract

Section: Discussionmentioning

confidence: 99%

Comprehensive In Silico Characterization and Expression Profiling of TCP Gene Family in Rapeseed

et al. 2021

View full text Add to dashboard Cite

show abstract

“…genic Arabidopsis by increasing primary root length at the vegetative growth stage [52]. In Vigna unguiculata, a drought-and heat-tolerant legume crop, the transgenic lines that overexpressed VuTCP9 displayed higher tolerance to salinity stress through regulation at physiological and biochemical levels, as well as phenotypic alterations [53].…”

Section: Discussionmentioning

confidence: 99%

BpTCP3 Transcription Factor Improves Salt Tolerance of Betula platyphylla by Reducing Reactive Oxygen Species Damage

Ren

Jiang

et al. 2021

Forests

View full text Add to dashboard Cite

The plant-specific transcription factors TEOSINTE BRANCHED1/CYCLO IDEA/PROLIFERATING CELL FACTOR1 (TCP) act as developmental regulators that have many roles in the growth and development processes throughout the entire life span of plants. TCP transcription factors are responsive to endogenous and environmental signals, such as salt stress. However, studies on the role of the TCP genes in salt stress response have rarely focused on woody plants, especially forest trees. In this study, the BpTCP3 gene, a CYC/TB1 subfamily member, isolated from Betula platyphylla Sukaczev, was significantly influenced by salt stress. The β-glucuronidase (GUS) staining analysis of transgenic B. platyphylla harboring the BpTCP3 promoter fused to the reporter gene GUS (pBpTCP3::GUS) further confirmed that the BpTCP3 gene acts a positive regulatory position in salt stress. Under salt stress, we found that the BpTCP3 overexpressed lines had increased relative/absolute high growth but decreased salt damage index, hydrogen peroxide (H2O2), and malondialdehyde (MDA) levels versus wild-type (WT) plants. Conversely, the BpTCP3 suppressed lines exhibited sensitivity to salt stress. These results indicate that the BpTCP3 transcription factor improves the salt tolerance of B. platyphylla by reducing reactive oxygen species damage, which provides useful clues for the functions of the CYC/TB1 subfamily gene in the salt stress response of B. platyphylla.

show abstract

“…The treated A. thaliana individuals whose radicles were able to breach the seed coat within 24 h were considered as successfully germinated seeds. The experiment was repeated three times ( Ranal and Santana, 2006 ; Xu et al., 2021b ). The germination percentage (GP, %) was calculated using the following equation.…”

Section: Methodsmentioning

confidence: 99%

LbMYB48 positively regulates salt gland development of Limonium bicolor and salt tolerance of plants

Han

Qiao²,

Li³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Limonium bicolor is a dicotyledonous recretohalophyte with several multicellular salt glands on the leaves. The plant can directly secrete excess salt onto the leaf surface through the salt glands to maintain ion homeostasis under salt stress. Therefore, it is of great significance to study the functions of genes related to salt gland development and salt tolerance. In this study, an R1-type MYB transcription factor gene was screened from L. bicolor, named LbMYB48, and its expression was strongly induced by salt stress. Subcellular localization analysis showed that LbMYB48 was localized in the nucleus. LbMYB48 protein has transcriptional activation activity shown by transcriptional activation experiments. The density of salt glands in the leaves and the salt secretion capacity of LbMYB48-silenced lines were decremented, as demonstrated by the leaf disc method to detect sodium ion secretion. Furthermore, salt stress index experiments revealed that the ability of LbMYB48-silenced lines to resist salt stress was significantly reduced. LbMYB48 regulates salt gland development and salt tolerance in L. bicolor mainly by regulating the expression of epidermal cell development related genes such as LbCPC-like and LbDIS3 and salt stress-related genes (LbSOSs, LbRLKs, and LbGSTs) as demonstrated by RNA-seq analysis of LbMYB48-silenced lines. The heterologous over-expression of LbMYB48 in Arabidopsis thaliana improves salt tolerance of plants by stabilizing ion and osmotic balance and is likely to be involved in the abscisic acid signaling pathway. Therefore, LbMYB48, a transcriptional activator regulates the salt gland development of L. bicolor and salt tolerance of L. bicolor and A. thaliana.

show abstract

The TCP transcription factor PeTCP10 modulates salt tolerance in transgenic Arabidopsis

Cited by 41 publications

References 93 publications

Comprehensive In Silico Characterization and Expression Profiling of TCP Gene Family in Rapeseed

Comprehensive In Silico Characterization and Expression Profiling of TCP Gene Family in Rapeseed

BpTCP3 Transcription Factor Improves Salt Tolerance of Betula platyphylla by Reducing Reactive Oxygen Species Damage

LbMYB48 positively regulates salt gland development of Limonium bicolor and salt tolerance of plants

Contact Info

Product

Resources

About