The theoretical consideration of microgravity effects on a cell

Kondrachuk, A. V.; Sirenko, S.P.

doi:10.1016/0273-1177(95)00630-w

Cited by 6 publications

(2 citation statements)

References 2 publications

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“…In case of fibroblasts, mechanical stress is an important regulator of distinct cell shape and extracellular matrix component production [Croute et al, 1995;Seitzer et al, 1995;Buravkova and Romanov, 2001]. Some more theoretical studies have argued against cells sensing gravity or changes therein [Kondrachuk and Sirenko, 1996;Kondrachuk, 2001]. Nevertheless, a limited number of studies has been performed to examine cell shape changes under various gravity conditions [Rijken et al, 1994;Cogoli and Cogoli-Greuter, 1997;Boonstra, 1999;Sciola et al, 1999;Searby et al, 2005;Erkut and Can, 2005].…”

Section: Introductionmentioning

confidence: 98%

The effect of combined hypergravity and microgrooved surface topography on the behaviour of fibroblasts

Loesberg

Walboomers

Loon

et al. 2006

Cell Motil. Cytoskeleton

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This study evaluated in vitro the differences in morphological behaviour between fibroblast cultured on smooth and micro-grooved substrata (groove depth: 1 mum, width: 1, 2, 5, 10 microm), which undergo artificial hypergravity by centrifugation (10, 24 and 50 g; or 1 g control). The aim of the study was to clarify which of these parameters was more important to determine cell behaviour. Morphological characteristics were investigated using scanning electron microscopy and fluorescence microscopy in order to obtain qualitative information on cell spreading and alignment. Confocal laser scanning microscopy visualised distribution of actin filaments and vinculin anchoring points through immunostaining. Finally, expression of collagen type I, fibronectin, and alpha(1)- and beta(1)-integrin were investigated by PCR. Microscopy and image analysis showed that the fibroblasts aligned along the groove direction on all textured surfaces. On the smooth substrata (control), cells spread out in a random fashion. The alignment of cells cultured on grooved surfaces increased with higher g-forces until a peak value at 25 g. An ANOVA was performed on the data, for all main parameters: topography, gravity force, and time. In this analysis, all parameters proved significant. In addition, most gene levels were reduced by hypergravity. Still, collagen type 1 and fibronectin are seemingly unaffected by time or force. From our data it is concluded that the fibroblasts primarily adjust their shape according to morphological environmental cues like substratum surface whilst a secondary, but significant, role is played by hypergravity forces.

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Section: Introductionmentioning

confidence: 98%

The effect of combined hypergravity and microgrooved surface topography on the behaviour of fibroblasts

Loesberg

Walboomers

Loon

et al. 2006

Cell Motil. Cytoskeleton

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“…it has a magnitude and direction. During an experimental run in the RPM the sample position with regard to the Earth's gravity vector direction is constantly changing, and as a result the samples will experience a near-weightlessness environment [Briegleb, 1992;Cogoli, 1992;Kessler, 1992;Sievers and Hejnowicz, 1992;Kondrachuk and Sirenko, 1996;Thery et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

The effect of combined simulated microgravity and microgrooved surface topography on fibroblasts

Loesberg

Walboomers

Bronkhorst

et al. 2007

Cell Motil. Cytoskeleton

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This study evaluated in vitro the differences in morphological behaviour between fibroblast cultured on smooth and microgrooved substrata (groove depth: 0.5 microm, width: 1, 2, 5, and 10 microm), which were subjected to simulated microgravity. The aim of the study was to clarify which of these parameters was more dominant to determine cell behaviour. Morphological characteristics were investigated using scanning electron microscopy and fluorescence microscopy in order to obtain qualitative information on cell alignment and area. Confocal laser scanning microscopy visualised distribution of actin filaments and focal adhesion points. Finally, expression of collagen type I, fibronectin, and alpha1- and beta1-integrin were investigated by PCR. Microscopy and image analysis showed that the fibroblasts aligned along the groove direction on all textured surfaces. On the smooth substrata, cells had spread out in a random fashion. The alignment of cells cultured on grooved surfaces decreased under simulated microgravity, especially after 24 h of culturing. Cell surface area on grooved substrata were significantly smaller than on smooth substrata, but simulated microgravity on the grooved groups resulted in an enlargement of cell area. ANOVA was performed on all main parameters: topography, gravity force, and time. In this analysis, all parameters proved significant. In addition, gene levels were reduced by microgravity particularly those of beta1-integrin and fibronectin. From our data it is concluded that the fibroblasts primarily adjust their shape according to morphological environmental cues like substratum surface whilst a secondary, but significant, role is played by microgravity conditions.

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Cellular and Molecular Responses to Gravitational Force-Triggered Stress in Cells of the Immune System

Ullrich

Thiel

2019

Stress Challenges and Immunity in Space

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The theoretical consideration of microgravity effects on a cell

Cited by 6 publications

References 2 publications

The effect of combined hypergravity and microgrooved surface topography on the behaviour of fibroblasts

The effect of combined hypergravity and microgrooved surface topography on the behaviour of fibroblasts

The effect of combined simulated microgravity and microgrooved surface topography on fibroblasts

Cellular and Molecular Responses to Gravitational Force-Triggered Stress in Cells of the Immune System

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