The three Bacillus anthracis toxin genes are coordinately regulated by bicarbonate and temperature

Sirard, Jean‐Claude; Mock, Michèle; Fouet, Agnès

doi:10.1128/jb.176.16.5188-5192.1994

Cited by 127 publications

(141 citation statements)

References 21 publications

Supporting

Mentioning

135

Contrasting

Order By: Relevance

“…5,7,[9][10][11][12][13][14][15][16][17][18] Maximum production of toxins occurs during the transition from log to the stationary phase of growth, suggesting growth phase-regulation of expression. 19 Quorum-sensing (QS) is a process by which bacteria regulate the expression of density-and growth phase-dependent genes. [20][21][22][23][24][25] QS involves the synthesis, release and detection of small signaling molecules, termed auto-inducers.…”

Section: Introductionmentioning

confidence: 99%

Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Jones¹,

Peterson²,

Benn³

et al. 2010

Virulence

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Jones¹,

Peterson²,

Benn³

et al. 2010

Virulence

View full text Add to dashboard Cite

“…In B. anthracis, transcription of the genes encoding the virulence-associated capsule and anthrax tripartite toxin is activated by bicarbonate (Bartkus and Leppla, 1989;Cataldi et a/., 1992;Koehler et a/., 1994;Sirard et a/., 1994;Uchida et a/., 1993a), and bicarbonate-stimulated expression of both toxin and capsule genes is dependent on the trans-acting factors of atxA and acpA, respectively (Dai eta/., 1995;Vietri eta/., 1995). Because synthesis of these virulence factors responds to the same signal, bicarbonate, it seemed likely that there might exist some interaction or cross-talk between the two regulatory systems.…”

Section: Discussionmentioning

confidence: 99%

Cross‐talk to the genes for Bacillus anthracis capsule synthesis by atxA, the gene encoding the frans‐activator of anthrax toxin synthesis

Uchida

Makino

Sekizaki

et al. 1997

Molecular Microbiology

View full text Add to dashboard Cite

SummaryThe two major virulence factors of Bacillus anfhracis are the tripartite toxin and the polyglutamate capsule, which are encoded by genes on the large plasmids, pXOl and pX02, respectively. The genes atxA, located on pXO1, and acpA, located on pX02, encode positive trans-acting proteins that are involved in bicarbonatemediated regulation of toxin and capsule production, respectively. A derivative strain cured of pXOl produced less capsular substance than the parent strain harbouring both pXOl and pX02, and electroporation of the strain cured of pXOl with a plasmid containing the cloned afxA gene resulted in an increased level of capsule production. An acpA-null mutant was complemented by not only acpA but also the afxA gene. The cap region, which is essential for encapsulation, contains three genes capB, cape, and capA, arranged in that order. The afxA gene stimulated capsule synthesis from the cloned cap region. Transcriptional analysis of cap by RNA slot-blot hybridization and primer-extension analysis revealed that afxA activated expression of cap in trans at the transcriptional level. These results indicate that cross-talk occurs, in which the pXOl -located gene, atxA, activates transcription of the cap region genes located on pX02. We identified two major apparent transcriptional start sites, designated P1 and P2, located at positions 731 bp and 625 bp, respectively, upstream of the translationinitiation codon of caps. Transcription initiated from P1 and P2 was activated by both atxA and acpA, and activation appeared to be stimulated by bicarbonate. Deletion analysis of the upstream region of the cap

show abstract

“…B.anthracis mostly constitutes two major toxins which are composed of three different proteins, protective antigen, lethal factor and edema factor. The structural genes for these components are located on the virulent plasmid pXO1 [5]. The plasmid pXO2 encodes an antiphagocytic capsule composed of a high molecular-weight polypeptide (poly-D-glutamic acid) which inhibits the host phagocytosis of the vegetative form of B.anthracis.…”

Section: Introductionmentioning

confidence: 99%

Virtual Screening and Docking Studies of Identified Potential Drug Target: Polysaccharide Deacetylase in <i>Bacillus anthracis</i>

Zaveri

Chaitanya

Reddy

2015

ILNS

View full text Add to dashboard Cite

In recent years, insilico approaches have been predicting novel drug targets. The present day development in pharmaceutics mainly ponders on target based drugs and this has been aided by structure based drug designing and subtractive genomics. In the present study, the computational genome subtraction methodology was applied for identification of novel, potential drug target against Bacillus anthracis, cause of deadly anthrax. The potential drug target identified through subtractive genomics approach was considered as polysaccharide deacetylase. By virtual screening against NCI database and Drugbank chemical libraries, two potential lead molecules were predicted. Further the potential lead molecules and target protein were subjected for docking studies using Autodock.

show abstract

The three Bacillus anthracis toxin genes are coordinately regulated by bicarbonate and temperature

Cited by 127 publications

References 21 publications

Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Cross‐talk to the genes for Bacillus anthracis capsule synthesis by atxA, the gene encoding the frans‐activator of anthrax toxin synthesis

Virtual Screening and Docking Studies of Identified Potential Drug Target: Polysaccharide Deacetylase in <i>Bacillus anthracis</i>

Contact Info

Product

Resources

About