The Tip-Localized Phosphatidylserine Established by Arabidopsis ALA3 Is Crucial for Rab GTPase-Mediated Vesicle Trafficking and Pollen Tube Growth

Zhou, Yuelong; Yang, Yang; Niu, Yue; Fan, Tingting; Qian, Dong; Luo, Changxin; Shi, Yumei; Li, Shanwei; An, Lizhe; Xiang, Yun

doi:10.1105/tpc.19.00844

Cited by 46 publications

(47 citation statements)

References 71 publications

(109 reference statements)

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“…Under normal growth conditions, seedlings grown on 1% agar MS plates for 5 days were treated with or without 4°C for the duration shown in the figure for fluorescence acquisition. Images of epidermal cells from root transition and elongation zone were acquired using a spinning disk microscope (Andor) equipped with a 63 × NA oil immersion lens, and the Z-series images were captured with the step size set at 0.5 μm (Zhou et al, 2020 ). GFP was excited with a 488-nm laser and observed using a 514-nm emission filter.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress

Zhang

Qian

Luo

et al. 2021

Front. Cell Dev. Biol.

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Low temperature is a major adverse environment that affects normal plant growth. Previous reports showed that the actin cytoskeleton plays an important role in the plant response to low-temperature stress, but the regulatory mechanism of the actin cytoskeleton in this process is not clear. C-repeat binding factors (CBFs) are the key molecular switches for plants to adapt to cold stress. However, whether CBFs are involved in the regulation of the actin cytoskeleton has not been reported. We found that Arabidopsis actin depolymerizing factor 5 (ADF5), an ADF that evolved F-actin bundling function, was up-regulated at low temperatures. We also demonstrated that CBFs bound to the ADF5 promoter directly in vivo and in vitro. The cold-induced expression of ADF5 was significantly inhibited in the cbfs triple mutant. The freezing resistance of the adf5 knockout mutant was weaker than that of wild type (WT) with or without cold acclimation. After low-temperature treatment, the actin cytoskeleton of WT was relatively stable, but the actin cytoskeletons of adf5, cbfs, and adf5 cbfs were disturbed to varying degrees. Compared to WT, the endocytosis rate of the amphiphilic styryl dye FM4-64 in adf5, cbfs, and adf5 cbfs at low temperature was significantly reduced. In conclusion, CBFs directly combine with the CRT/DRE DNA regulatory element of the ADF5 promoter after low-temperature stress to transcriptionally activate the expression of ADF5; ADF5 further regulates the actin cytoskeleton dynamics to participate in the regulation of plant adaptation to a low-temperature environment.

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Section: Methodsmentioning

confidence: 99%

Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress

Zhang

Qian

Luo

et al. 2021

Front. Cell Dev. Biol.

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“…Of the numerous Rabs identified in our study, members of the RabA1, D2, and E1 families were most abundant (Figure 5C; Supplemental Dataset 4). RabA proteins are most closely related to mammalian Rab11, which regulates endosomal trafficking and mediate TGN-PM trafficking in plants (Zhou, et al, 2020;Li, et al, 2017;Nielsen et al, 2008). In addition, members of the RabE family, which are orthologs of the Sec4p/Rab8 GTPase family, mediate exocytosis in yeast and mammalian cells (Rutherford and Moore, 2002) and regulate post-Golgi trafficking to the PM and to the cell plate during cytokinesis (Orr, et al, 2021;Speth et al, 2009;Ahn et al, 2013).…”

Section: Small Gtpasesmentioning

confidence: 99%

Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant Specific Components

Dahhan¹,

Reynolds²,

Cárdenas³

et al. 2021

Preprint

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In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from the cell surface as well as the movement of cargo in post-Golgi trafficking pathways. This diversity of functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment and cargo selectivity. The adaptor-protein AP-1 complex operates as part of the secretory pathway at the trans-Golgi network, while the AP-2 complex and the TPLATE complex (TPC) jointly operate at the plasma membrane to execute clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated trafficking in plants will be the comprehensive identification and characterization of the network of evolutionarily conserved and plant-specific core and accessory machinery involved in the formation and targeting of CCVs. To facilitate these studies, we have analyzed the proteome of enriched trans-Golgi network/early endosome-derived and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis cells. Tandem mass spectrometry analysis results were validated by differential chemical labeling experiments to identify proteins co-enriching with CCVs. Proteins enriched in CCVs included previously characterized CCV components and cargos such as the vacuolar sorting receptors in addition to conserved and plant-specific components whose function in clathrin-mediated trafficking has not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis CCVs is further supported via additional biochemical data.

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“…Phosphatidylserine (PS) is abundant in the inverted-cone zone of the apical pollen tube in Arabidopsis . Recent research has revealed that loss of apical localization of PS and significantly decreased distribution lead to obvious decreases in vesicle numbers and an obvious increase in pollen tube width, which indicates that tip-localized PS establishment is important for vesicle targeting/trafficking and polar growth of pollen tubes in Arabidopsis ( Zhou et al, 2020 ).…”

Section: Vesicle Deliverymentioning

confidence: 99%

Secretory Vesicles Targeted to Plasma Membrane During Pollen Germination and Tube Growth

Ruan

Jiang

Wang

et al. 2021

Front. Cell Dev. Biol.

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Pollen germination and pollen tube growth are important biological events in the sexual reproduction of higher plants, during which a large number of vesicle trafficking and membrane fusion events occur. When secretory vesicles are transported via the F-actin network in proximity to the apex of the pollen tube, the secretory vesicles are tethered and fused to the plasma membrane by tethering factors and SNARE proteins, respectively. The coupling and uncoupling between the vesicle membrane and plasma membrane are also regulated by dynamic cytoskeleton, proteins, and signaling molecules, including small G proteins, calcium, and PIP2. In this review, we focus on the current knowledge regarding secretory vesicle delivery, tethering, and fusion during pollen germination and tube growth and summarize the progress in research on how regulators and signaling molecules participate in the above processes.

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The Tip-Localized Phosphatidylserine Established by Arabidopsis ALA3 Is Crucial for Rab GTPase-Mediated Vesicle Trafficking and Pollen Tube Growth

Abstract: PS regulates polar growth of pollen tubes One-sentence summary: Arabidopsis ALA3 plays a key role in the inverted-cone distribution of PS at the pollen tube tip, and PS is crucial for the distribution and activity of certain Rab GTPases as well as pollen tube growth.

Cited by 46 publications

References 71 publications

Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress

Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress

Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant Specific Components

Secretory Vesicles Targeted to Plasma Membrane During Pollen Germination and Tube Growth

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