The Toxicity of Extracts With Bimetallic Nanoparticles on Ferns Spores Germination

Abstract

Plants based synthesis of Ag and Au nanoparticles represent one of the most important focus of researchers due to their applications and catalytic activities. We utilized various techniques to obtain extracts with bimetallic nanoparticles from Asplenium scolopendrium leaves; we tested the following variants: M -microwave extract, M NP -microwave extract with nanoparticles Au-Ag, C -extract obtained in the oven, C NP -extract obtained in the oven with nanoparticles Au-Ag, H -hydroalcoholic solution; for each extract were made 2 dilutions: 1:10 and 1:100. The germinative percent was used to quantify the effect of the extracts with/without bimetallic nanoparticles on Asplenium scolopendrium and Dryopteris filix-mas spores. In the variants with bimetallic nanoparticles, regardless species, dilution or obtaining technique, after 1 week no germination was recorded and after one month the situation was the same. Also, no germination was observed in the variants without bimetallic nanoparticles with 1:10 dilution. In both species, at 1:100 dilution, the variants with the extract obtained using microwave technique (without nanoparticles) recorded a higher germinative percent than the other variants with extract.