The transfluthrin effect on mosquito repellent to sperm quality and testicular histology of rats

Permatasari, Silvani; Frethernety, Agnes; Shinta, Herlina Eka

doi:10.1063/5.0103917

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…These antioxidant properties work naturally to help ward off free radicals or ROS in the testes so that the mitochondrial cell membrane is not disturbed, so that ATP supplies become abundant and cause increased sperm motility. 23,24…”

Section: Discussionmentioning

confidence: 99%

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

Permatasari,

Syarpin,

Pujianto

2023

JKKI

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Background: Saluang belum (Lavanga sarmentosa) is one of Kalimantan's typical plants, which is as efficacious as a traditional medicine to increase sexual activity and male fertility. Based on previous studies, the content of flavonoid and steroid in 70% ethanol extract of L. sarmentosa were able to affect sperm quality of mice. Studies related to L. sarmentosa are still limited for phytochemical test and their bioactivity on human spermatozoa motility in vitro.Objective: This study is to perform phytochemical tests of compound content in fractionation with eluents of high and low polarity, namely methanol and chloroform, and then to test their bioactivity on the motility of human spermatozoa in vitro.Methods: L. sarmentosa was extracted with 96% ethanol and fractionated by using a vacuum chromatography column with chloroform and methanol as the eluent. Then obtained samples were analysed by a quantitative phytochemical test. The samples used in-vitro human spermatozoa were divided into eleven groups: control group, group administered with L. sarmentosa extract eluent chloroform of 10, 50, 100, 500, and 1000 ng/mL, and same concentration with extract eluent methanol. Furthermore, the sperm motility was analysed by using a Computer Assisted Sperm Analyser (CASA). Results: The methanol and chloroform fraction of L. sarmentosa root extract contained metabolites, namely terpenoids, flavonoids, steroids, alkaloids, saponins, and tannins. The sperm motility increased significantly at the treatment group of the methanol and chloroform fractions compared to the control group. There was a significant difference between the sperm motility incubated with methanol and that with chloroform fraction at concentration 500 and 1000 ng/mL.Conclusion: The results of sperm motility were higher in the methanol fraction than those in the chloroform fraction.

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Section: Discussionmentioning

confidence: 99%

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

Permatasari,

Syarpin,

Pujianto

2023

JKKI

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“…The calculations were based on 200 sperm cells, colourless living spermatozoa, and red dead spermatozoa from its head. The viability observation result was given in percentages (Permatasari et al, 2023). Mice histology test was done using the right testicle which was placed in a container of 10% Neutral Buffer Formalin.…”

Section: Effects Of Saluang Belum Root Infusion On Sperm Motility Via...mentioning

confidence: 99%

“…Flavonoids can neutralise free radicals in the mitochondria to protect and maximise ATP production needed to optimise sperm production. Additionally, ATP can be transferred to the sperm tail to aid in its motility (Syarif et al, 2016;Permatasari et al, 2023).…”

Section: Effect Of Saluang Belum On the Sperms Of Mus Musculusmentioning

confidence: 99%

The effects of saluang belum (Luvunga sarmentosa) root infusion on sperm motility, viability, and testicle histology in mice (Mus musculus)

Permatasari,

Christin,

Rahmatina

et al. 2024

Pharm Educ

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Background: Men's infertility is frequently caused by a decrease in sperm quality. Herbal medicine, which is relatively cheap and easy to obtain, can be used to treat this condition. People in Central Borneo frequently consume boiled Saluang Belum root (Luvunga sarmentosa). L. sarmentosa root has the potential to improve sperm quality. Objective: To investigate the effects of Saluang Belum root infusion on sperm cell survival and testicle histology in mice (Mus musculus). Method: L. sarmentosa root was extracted using an infusion method, analysed and administered to mice at doses of 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW. Aquadest was used as a negative control for 15 days. On the 16th day, sperm motility and viability were assessed. The right testicles were removed and examined under a microscope at 100x and 400x magnification for five fields of view to examine mice testicle histology. Result: Infusion of L. sarmentosa contained triterpenoids, flavonoids, steroids, alkaloids, saponins, and phenols. Statistical tests of infused L. sarmentosa root on the motility, viability sperm and spermatogenic potentials of mice indicated that the doses of 200mg/kgBW, 400mg/kgBW, and 600mg/kgBW are significantly increased than control. Conclusion: L. sarmentosa root infusion increased sperm motility and viability, and the spermatogenic cells, as well as Leydig cells in mice.

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The transfluthrin effect on mosquito repellent to sperm quality and testicular histology of rats

Cited by 2 publications

References 9 publications

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

The effects of saluang belum (Luvunga sarmentosa) root infusion on sperm motility, viability, and testicle histology in mice (Mus musculus)

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