The Triple-Repeat Protein Anakonda Controls Epithelial Tricellular Junction Formation in Drosophila

Byri, Sunitha; Misra, Tvisha; Syed, Zulfeqhar A.; Bätz, Tilmann; Shah, Jimit; Boril, Lukas; Glashauser, Jade; Aegerter-Wilmsen, Tinri; Matzat, Till; Moussian, Bernard; Uv, Anne; Luschnig, Stefan

doi:10.1016/j.devcel.2015.03.023

Cited by 80 publications

(109 citation statements)

References 52 publications

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“…3A-D), suggesting that Gli is a component of bicellular SJs in these epithelia of larval A. aegypti. This observation is inconsistent with D. melanogaster Gli, which is reported to be concentrated at the TCJs in the midgut epithelium (Byri et al, 2015). However, in the epithelia of epidermis and wing imaginal discs, D. melanogaster Gli spreads throughout the entire SJ domain and interacts with other bicellular SJ components when overexpressed (Schulte et al, 2006).…”

Section: Gli Expression and Localization In The Osmoregulatory Organscontrasting

confidence: 54%

“…2A). The expression of Gli in a wide range of epithelial tissues, including the midgut and hindgut, has been shown in larval D. melanogaster (Schulte et al, 2003(Schulte et al, , 2006Byri et al, 2015). In contrast, Gli is not expressed in the D. melanogaster Malpighian tubules (Schulte et al, 2006).…”

Section: Gli Expression and Localization In The Osmoregulatory Organsmentioning

confidence: 99%

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Identification of the septate junction protein gliotactin in the mosquito, Aedes aegypti: evidence for a role in increased paracellular permeability in larvae

Jonusaite

Kelly

Donini

2017

Journal of Experimental Biology

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Septate junctions (SJs) regulate paracellular permeability across invertebrate epithelia. However, little is known about the function of SJ proteins in aquatic invertebrates. In this study, a role for the transmembrane SJ protein gliotactin (Gli) in the osmoregulatory strategies of larval mosquito (Aedes aegypti) was examined. Differences in gli transcript abundance were observed between the midgut, Malpighian tubules, hindgut and anal papillae of A. aegypti, which are epithelia that participate in larval mosquito osmoregulation. Western blotting of Gli revealed its presence in monomer, putative dimer and alternatively processed protein forms in different larval mosquito organs. Gli localized to the entire SJ domain between midgut epithelial cells and showed a discontinuous localization along the plasma membranes of epithelial cells of the rectum as well as the syncytial anal papillae epithelium. In the Malpighian tubules, Gli immunolocalization was confined to SJs between the stellate and principal cells. Rearing larvae in 30% seawater caused an increase in Gli protein abundance in the anterior midgut, Malpighian tubules and hindgut. Transcriptional knockdown of gli using dsRNA reduced Gli protein abundance in the midgut and increased the flux rate of the paracellular permeability marker, polyethylene glycol (molecular weight 400 Da; PEG-400). Data suggest that in larval A. aegypti, Gli participates in the maintenance of salt and water balance and that one role for Gli is to participate in the regulation of paracellular permeability across the midgut of A. aegypti in response to changes in environmental salinity.

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Section: Gli Expression and Localization In The Osmoregulatory Organscontrasting

confidence: 54%

Section: Gli Expression and Localization In The Osmoregulatory Organsmentioning

confidence: 99%

Identification of the septate junction protein gliotactin in the mosquito, Aedes aegypti: evidence for a role in increased paracellular permeability in larvae

Jonusaite

Kelly

Donini

2017

Journal of Experimental Biology

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“…Vertebrates, including Xenopus , have TJs and AJs, whereas invertebrates, including Drosophila , have AJs and septate junctions [54, 55], which serve an analogous function to TJs while differing in ultrastructure. Likewise, the ultrastructure of tricellular junctions is distinct between vertebrates and Drosophila [56]. …”

Section: Discussionmentioning

confidence: 99%

Maintenance of the Epithelial Barrier and Remodeling of Cell-Cell Junctions during Cytokinesis

et al. 2016

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Summary Epithelial integrity and barrier function must be maintained during the complex cell shape changes that occur during cytokinesis in vertebrate epithelial tissue. Here, we investigate how adherens junctions and bicellular and tricellular tight junctions are maintained and remodeled during cell division in the Xenopus laevis embryo. We find that epithelial barrier function is not disrupted during cytokinesis and is mediated by sustained tight junctions. Using FRAP, we demonstrate that adherens junction proteins are stabilized at the cleavage furrow by increased tension. We find that vinculin is recruited to the adherens junction at the cleavage furrow, and inhibiting recruitment of vinculin by expressing a dominant negative mutant increases the rate of furrow ingression. Furthermore, we show that cells neighboring the cleavage plane are pulled between the daughter cells, making a new interface between neighbors, and two new tricellular tight junctions flank the midbody following cytokinesis. Our data provide new insight into how epithelial integrity and barrier function are maintained throughout cytokinesis in vertebrate epithelial tissue.

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“…In freeze-fracture images, the rows of intramembrane particles in pSJs are separated from one another, whereas those in sSJs are fused into ridges (Lane et al, 1994;Lane and Swales, 1982). To date, more than 20 pSJ-related proteins, including pSJ components and regulatory proteins involved in pSJ assembly, have been identified and characterized in Drosophila (Banerjee et al, 2006;Byri et al, 2015;Deligiannaki et al, 2015;Hildebrandt et al, 2015;Izumi and Furuse, 2014;Tepass et al, 2001;Wu and Beitel, 2004). By contrast, few genetic and molecular analyses have been carried out on sSJs.…”

Section: Introductionmentioning

confidence: 99%

A tetraspanin regulates septate junction formation in Drosophila midgut

Izumi¹,

Motoishi²,

Furuse³

et al. 2016

Development

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Septate junctions (SJs) are membrane specializations that restrict the free diffusion of solutes through the paracellular pathway in invertebrate epithelia. In arthropods, two morphologically different types of septate junctions are observed; pleated (pSJs) and smooth (sSJs), which are present in ectodermally and endodermally derived epithelia, respectively. Recent identification of sSJ-specific proteins, Mesh and Ssk, in Drosophila indicates that the molecular compositions of sSJs and pSJs differ. A deficiency screen based on immunolocalization of Mesh identified a tetraspanin family protein, Tsp2A, as a newly discovered protein involved in sSJ formation in Drosophila. Tsp2A specifically localizes at sSJs in the midgut and Malpighian tubules. Compromised Tsp2A expression caused by RNAi or the CRISPR/Cas9 system was associated with defects in the ultrastructure of sSJs, changed localization of other sSJ proteins, and impaired barrier function of the midgut. In most Tsp2A mutant cells, Mesh failed to localize to sSJs and was distributed through the cytoplasm. Tsp2A forms a complex with Mesh and Ssk and these proteins are mutually interdependent for their localization. These observations suggest that Tsp2A cooperates with Mesh and Ssk to organize sSJs.

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The Triple-Repeat Protein Anakonda Controls Epithelial Tricellular Junction Formation in Drosophila

Cited by 80 publications

References 52 publications

Identification of the septate junction protein gliotactin in the mosquito, Aedes aegypti: evidence for a role in increased paracellular permeability in larvae

Identification of the septate junction protein gliotactin in the mosquito, Aedes aegypti: evidence for a role in increased paracellular permeability in larvae

Maintenance of the Epithelial Barrier and Remodeling of Cell-Cell Junctions during Cytokinesis

A tetraspanin regulates septate junction formation in Drosophila midgut

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