1987
DOI: 10.1530/acta.0.114s157
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The TSH receptor: Structure and interaction with autoantibodies in thyroid disease

Abstract: Studies of the TSH receptor using affinity labelling with photoactive derivatives of TSH and analysis by SDS-PAGE have shown that the receptor contains 2 subunits (A and B), linked by a disulphide bridge. Similar results are obtained with TSH receptors from human, porcine and guinea pig thyroid tissue and from guinea pig fat. Analysis of affinity labelled receptors under non-denaturing conditions suggest that subunits additional to the A and B subunits are not present.Hydrodynamic measurements indicate that th… Show more

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Cited by 12 publications
(9 citation statements)
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“…Interestingly, while the LH/CG receptor is expressed in target organs as a monomer (14), the TSH receptor is expressed in thyroid membranes as a heterodimer: an extracellular ␣ subunit (ϳ53 kDa) and a membrane-spanning ␤ subunit (ϳ38 kDa) are held together by disulfide bridge(s) (15). This observation is in agreement with one of the models proposed for the structure of the TSH receptor before the cloning (16). The post-translational cleavage in two subunits is almost complete in human thyroid tissue, whereas in L cells stably transfected with the TSH receptor, a small amount of uncleaved mature receptor may still be present.…”
supporting
confidence: 84%
See 1 more Smart Citation
“…Interestingly, while the LH/CG receptor is expressed in target organs as a monomer (14), the TSH receptor is expressed in thyroid membranes as a heterodimer: an extracellular ␣ subunit (ϳ53 kDa) and a membrane-spanning ␤ subunit (ϳ38 kDa) are held together by disulfide bridge(s) (15). This observation is in agreement with one of the models proposed for the structure of the TSH receptor before the cloning (16). The post-translational cleavage in two subunits is almost complete in human thyroid tissue, whereas in L cells stably transfected with the TSH receptor, a small amount of uncleaved mature receptor may still be present.…”
supporting
confidence: 84%
“…Cells were washed with ice-cold PBS, scraped, centrifuged at 1500 ϫ g for 5 min, and stored at Ϫ70°C. An aliquot of the Triton X-100 cellular extract (16) was used for cellular TSHR (cTSHR) determinations or protein content quantification using the Pierce BCA protein assay reagent kit (Pierce).…”
Section: Culture Of An L Cell Line Permanently Expressing the Human Tshmentioning
confidence: 99%
“…Furmaniak et al (40) have described a patient with hyperthyroid Graves' disease in which the TRAb changed from stimulating to blocking following 131 I treatment and then returned to stimulating 1 yr later. The patient's serum thyroid microsomal antibodies also showed interesting changes.…”
Section: Trab With Blocking Activitymentioning
confidence: 99%
“…The TSH receptor is present on the cell surface in very small amounts (in the region of 10 3 -10 4 sites per cell) (40,96) and, consequently, special techniques are often required to study the receptor. One of the most powerful techniques currently available for studying trace amounts of cell surface receptors is affinity labeling (97).…”
Section: A Photoaffinity Labelingmentioning
confidence: 99%
“…Since this subject has been covered extensively by Rees and colleagues ( [34], and elsewhere in this volume) the TSHR will not be discussed further in this article. Since this subject has been covered extensively by Rees and colleagues ( [34], and elsewhere in this volume) the TSHR will not be discussed further in this article.…”
Section: Introduction -The Thyroid Antigensmentioning
confidence: 99%