The two-dimensional migration pattern of the single HLA-DR beta chain expressed in DRw8 haplotypes is not fully predictive of its activity in antigen presentation

Bouillot, M; Choppin, Jeannine; Sterkers, Ghislaine; Freidel, C; Gebuhrer, L.; Bétuel, H; Lévy, J. P.

doi:10.1007/bf00372528

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…The heterogeneity of the HLA-DRw8 specificity has been reported from serological, cellular, and biochemical analyses (Bdtuel et al, 1984;Bouillot et al, 1988;Layrisse et aL, 1984;Mickelson et aL, 1983). These data suggest that there are differences in DR/3 chain sequences between the different DRw8 subspecifieities.…”

Section: Introductionmentioning

confidence: 72%

Direct sequencing of a HLA-DRB gene by polymerase chain reaction: Sequence variation in DRw8 specificity

et al. 1990

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SummaryThe nucleotide sequence of a HLA-DRB gene with a predominant subtype of DRw8 specificity in Japanese (DRS.1) was determined with single-stranded DNA enzymatically amplified by polymerase chain reaction (PCR). The sequence differs at a single amino acid from both of the published DRwS/DwS.1 and DRwS/Dw8.2 sequences: isoleucine67(ATC) instead of phenylalanine87(TTC) in DRwS/DwS.1 and serineSr(AGC) instead of aspartic acidaT(GAT) in DRw8/Dw8.2. On the other hand the DR8.1 and DRwS/DwS.3 have the same amino acid sequence although one silent nucleotide substitution has occurred between the two sequences. These results indicate that Japanese DR8.1 specificity corresponds to DRwS/Dw8.3. Furthermore, an oligonucleotide probe specific for this sequence was synthesized and hybridized with 33 HLAtyped controls. This probe clearly distinguished the particular subtype from other DRw8 subtypes and specificities.

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Section: Introductionmentioning

confidence: 72%

Direct sequencing of a HLA-DRB gene by polymerase chain reaction: Sequence variation in DRw8 specificity

et al. 1990

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A cellular and functional split in the DRw8 haplotype is due to a single amino acid replacement (DR ? ser 57- asp 57 )

1989

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The single DR beta chain gene of the DRw8 haplotype has been suggested to carry both the DRw8 and the DRw52 epitopes. Cellular typing has shown that the DRw8 haplotype can be split into three subtypes, Dw8.1, Dw8.2, and Dw8.3, presumably due to a polymorphism in the DRw8 beta chain. Furthermore, Dw8.1 and Dw8.2 cells present influenza virus antigen to different T-cell clones. In the present study, DRw8/Dw8.2 beta chain cDNA was cloned and characterized. A comparison of this sequence with a partial DRw8/Dw8.1 beta chain gene suggested that the DRw8 split is due to a single amino acid replacement of ser57-asp57 caused by three nucleotide substitutions in the same codon. In most DR haplotypes, two expressed DR beta chain genes exist. Comparing the nucleotide sequence of the single beta gene in the DRw8 haplotype to those of other DR beta genes revealed that the DRw8 beta gene sequence is most closely related to the DRB1 genes of the DR3, 5, and w6 haplotypes. However, the comparisons also showed that it was not possible from sequence similarities to divide the DR beta genes into two or more distinct allelic series.

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DNA typing for class II hla antigens with allele-specific or group-specific amplification. II. typing for alleles of the DRw52-associated group

1990

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The two-dimensional migration pattern of the single HLA-DR beta chain expressed in DRw8 haplotypes is not fully predictive of its activity in antigen presentation

Cited by 3 publications

References 15 publications

Direct sequencing of a HLA-DRB gene by polymerase chain reaction: Sequence variation in DRw8 specificity

Direct sequencing of a HLA-DRB gene by polymerase chain reaction: Sequence variation in DRw8 specificity

A cellular and functional split in the DRw8 haplotype is due to a single amino acid replacement (DR ? ser 57- asp 57 )

DNA typing for class II hla antigens with allele-specific or group-specific amplification. II. typing for alleles of the DRw52-associated group

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