The type-specific substance from Pneumococcus type 10A(34). The phosphodiester linkages

Abstract

1. The phosphate groups in the type-specific substance S. 10A from Pneumococcus type 10A (34) were shown to join the hydroxyl group at position 1 or 5 of ribitol and the hydroxyl group at position 5 or 6 of a d-galactofuranosyl residue in the next repeating unit. 2. A partial formula of the type-specific substance was derived.

The type-specific substance from Pneumococcus type 13

“…The glycerol and glycerophosphates are derived from the ribitol unit, acid hydrolysis of the phosphodiester linkage being random, as in S. 13 itself. The phosphate group is located on the L-1 (in preference to the D-1) position of the ribitol because of its presumed mode of biosynthesis from cytidine diphosphate ribitol, which contains the L-ribitol 1-phosphate moiety (Rao et al, 1966b;Baddiley, 1970).…”

Immunogenicity and immunochemistry ofStreptococcus pneumoniae capsular polysaccharides

“…Patients receiving the vaccine sub- (Perry et~. 1980) (17) (Rao et aL 1966) (18) (Richards eta, 1985) (19) (Kennedyetfl. 1969) (20) (Richards et al 1985) (21) (Richards et al 1985) Serotype (Jansson et al 1987) (Shabarova et al 1962) (30) (31) (Jansson et al 1983) (32) (Jansson et al, 1988a) (33) (Jansson et al, 1988b) (34) (Lugowski & Jennings 1984) (35) (Lee & Fraser 1980;Ohno et al 1980) (36) (Katzenellenbogen & Jennings 1983;Lee et al 1987) (37) (Lee & Fraser 1980;Lee et al 1987) (38) (Richards et al, 1983) …”

The type-specific substance from Pneumococcus type 10A(34). Structure of the dephosphorylated repeating unit

“…The low positive specific rotations of S. lOA, the neutral hexasaccharide and the basic hexasaccharide indicate that both o-and fl-linkages are present. The mode of linkage of the neutral hexasaccharide by phosphodiester groups is discussed in the next paper (Rao et al 1966).…”