The U1 snRNP Subunit LUC7 Modulates Plant Development and Stress Responses via Regulation of Alternative Splicing

Amorim, Marcella de Francisco; Willing, Eva Maria; Szabó, Emese; Francisco‐Mangilet, Anchilie G.; Droste-Borel, Irina; Maček, Boris; Schneeberger, Korbinian; Laubinger, Sascha

doi:10.1105/tpc.18.00244

Cited by 43 publications

(37 citation statements)

References 93 publications

(119 reference statements)

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“…In summary, we identified six sugar beet RBP which increased yeast salt tolerance upon expression. Characterization of two Arabidopsis orthologues of the sugar beet SATO have already proven that proteins coded by these genes ameliorate salt stress in plants [39,42]. We have provided additional evidence indicating that expression of BvSATO1 improves salt tolerance in Arabidopsis.…”

Section: Discussionmentioning

confidence: 63%

“…In our screening we identified BvSATO3, which is ortholog of the Arabidopsis U1snRPN accessory protein LUC7. It has been demonstrated that LUC7 is important for constitutive splicing, but also for alternative splicing events modulated by stress [42]. We also identified a second protein, BvU2AF35b, known to participate in mRNA splicing in plants and mammals.…”

Section: Discussionmentioning

confidence: 78%

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RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

et al. 2020

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Salt stress drastically reduce crop productivity. In order to identify genes that could improve crop salt tolerance, we randomly expressed a cDNA library of the halotolerant sugar beet in a sodium-sensitive yeast strain. We identified six sugar beet genes coding for RNA binding proteins (RBP) able to increase the yeast Na+-tolerance. Two of these genes, named Beta vulgaris Salt Tolerant 3 (BvSATO3) and BvU2AF35b, participate in RNA splicing. The other four BvSATO genes (BvSATO1, BvSATO2, BvSATO4 and BvSATO6) are putatively involved in other processes of RNA metabolism. BvU2AF35b improved the growth of a wild type yeast strain under salt stress, and also in mutant backgrounds with impaired splicing, thus confirming that splicing is a target of salt toxicity. To validate the yeast approach, we characterized BvSATO1 in sugar beet and Arabidopsis. BvSATO1 expression was repressed by salt treatment in sugar beet, suggesting that this gene could be a target of salt toxicity. Expression of BvSATO1 in Arabidopsis increased the plant salt tolerance. Our results suggest that not only RNA splicing, but RNA metabolic processes such as such as RNA stability or nonsense-mediated mRNA decay may also be affected by salt stress and could be biotechnological targets for crop improvement.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 78%

RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

et al. 2020

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“…AS is a conserved gene regulation in eukaryotes and has been thought to be involved in many biological processes. In recent years the AS regulation in stress has been reported (Laloum et al, 2018;de Francisco Amorim et al, 2018;Calixto et al, 2018;Shih et al, 2019). Here, we found that most DAS genes caused by insect damage were not affected at the transcription level.…”

Section: Discussionmentioning

confidence: 58%

“…Based on their amino acid sequence similarity, enzymatic activity, or other biological properties (van Loon et al, 2006;Breen et al, 2017), PR proteins are classified into 17 groups of which the PR1, PR2, PR3, PR4, and PR5 are the five groups discovered firstly (Kitajima and Sato, 1999). PR1 protein was discovered in tobacco in response to tobacco mosaic virus (TMV) infection (Cornelissen et al, 1986) and its homologs have been identified in barley, tomato, corn, and rice (Niderman et al, 1995;Liu and Xue, 2006). The PR1 family contains the most abundant PR proteins which are induced by pathogen infections (Breen et al, 2017).…”

Section: More Significance Induction Of Pr Genes By a Lucorummentioning

confidence: 99%

“…In plants, AS are found related to plant growth, development, and light morphology (Staiger and Brown, 2013;Hartmann et al, 2016). It also links to stress responses (Laloum et al, 2018;Calixto et al, 2018;de Francisco Amorim et al, 2018) and maintenance of the mineral nutrient homeostasis (Dong et al, 2018). JAZ proteins are the main repressors of the JA signaling (Chini et al, 2007;Sun et al, 2017;Howe et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests

Chen

Wang

et al. 2020

Front. Plant Sci.

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The green mirid bug (Apolygus lucorum) and the cotton bollworm (Helicoverpa armigera) are both preferred to live on cotton but cause different symptoms, suggesting specialized responses of cotton to the two insects. In this study, we investigated differential molecular mechanisms underlying cotton plant defenses against A. lucorum and H. armigera via transcriptomic analyses. At the transcription level, jasmonate (JA) signaling was dominated in defense against H. armigera whereas salicylic acid (SA) signaling was more significant in defense against A. lucorum. A set of pathogenesis-related (PR) genes and protease inhibitor genes were differentially induced by the two insects. Insect infestations also had an impact on alternative splicing (AS), which was altered more significantly by the H. armigera than A. lucorum. Interestingly, most differential AS (DAS) genes had no obvious change at the transcription level. GO analysis revealed that biological process termed "RNA splicing" and "cellular response to abiotic stimulus" were enriched only in DAS genes from the H. armigera infested samples. Furthermore, insect infestations induced the retained intron of GhJAZs transcripts, which produced a truncated protein lacking the intact Jas motif. Taken together, our data demonstrate that the specialized cotton response to different insects is regulated by gene transcription and AS as well.

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Proteomic analysis of near‐isogenic lines reveals key biomarkers on wheat chromosome 4B conferring drought tolerance

et al. 2023

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Drought is a major constraint for wheat production that is receiving increased attention due to global climate change. This study conducted isobaric tags for relative and absolute quantitation proteomic analysis on near‐isogenic lines to shed light on the underlying mechanism of qDSI.4B.1 quantitative trait loci (QTL) on the short arm of chromosome 4B conferring drought tolerance in wheat. Comparing tolerant with susceptible isolines, 41 differentially expressed proteins were identified to be responsible for drought tolerance with a p‐value of < 0.05 and fold change >1.3 or <0.7. These proteins were mainly enriched in hydrogen peroxide metabolic activity, reactive oxygen species metabolic activity, photosynthetic activity, intracellular protein transport, cellular macromolecule localization, and response to oxidative stress. Prediction of protein interactions and pathways analysis revealed the interaction between transcription, translation, protein export, photosynthesis, and carbohydrate metabolism as the most important pathways responsible for drought tolerance. The five proteins, including 30S ribosomal protein S15, SRP54 domain‐containing protein, auxin‐repressed protein, serine hydroxymethyltransferase, and an uncharacterized protein with encoding genes on 4BS, were suggested as candidate proteins responsible for drought tolerance in qDSI.4B.1 QTL. The gene coding SRP54 protein was also one of the differentially expressed genes in our previous transcriptomic study.

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The U1 snRNP Subunit LUC7 Modulates Plant Development and Stress Responses via Regulation of Alternative Splicing

Cited by 43 publications

References 93 publications

RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

Differential Transcription and Alternative Splicing in Cotton Underly Specialized Defense Responses Against Pests

Proteomic analysis of near‐isogenic lines reveals key biomarkers on wheat chromosome 4B conferring drought tolerance

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