The Unknown microRNA Expression of Male Breast Cancer. Similarities and Differences with Female Ductal Carcinoma. Their Role as Tumor Biomarker

Merino, María J.; Gil, Sara M.; Macías, Carmen García; Lara, Karlena

doi:10.7150/jca.23151

Cited by 10 publications

(8 citation statements)

References 38 publications

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“…microRNA (miRNA) is an endogenous non-protein-encoded small RNA, located in eukaryotic cells and approximately 22 nt in length. Studies have shown that its expression has a certain impact on the occurrence, development, invasion, metastasis and prognosis of many malignant tumors, which has an important effect in inhibiting tumors or promoting tumor development (8). miRNA has a great significance in the diagnosis and treatment evaluation of NSCLC and can be used as a non-invasive biomarker for prognosis (9).…”

Section: Introductionmentioning

confidence: 99%

High expression of miR-155 and miR-21 in the recurrence or metastasis of non-small cell lung cancer

Shi

2019

Oncol Lett

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High expression of miR-155 and miR-21 in the recurrence or metastasis of non-small cell lung cancer (NSCLC) was investigated. Retrospective analysis on the clinical information of 180 patients with NSCLC was carried out. The patients were admitted to Daqing Oil Field General Hospital from February 2012 to March 2015 and they were the research group. Moreover, the physical examination information of 88 normal medical examinees were selected at the same period of time as the control group. In the research group, 68 patients diagnosed with NSCLC were the newly diagnosed group and 112 cases of recurrence or metastasis of NSCLC were the recurrence group. The quantitative real-time polymerase chain reaction was used to detect the expression levels of serum miR-115 and miR-21. In addition, the expression levels between miR-155 and miR-21 and the relationship between the recurrence rate and metastasis of NSCLC were analyzed. The impact on the prognosis of patients were also analyzed. The expression levels of serum miR-155 and miR-21 were higher in the research group than those in the control group (P<0.05). The expression levels of serum miR-155 and miR-21 were higher in the recurrence group than those in the newly diagnosed group (P<0.05). We followed up the patients in the research group for 36 months, the median survival time and mortality rate in the recurrence group was higher than that of in the newly diagnosed group (χ 2 =9.705, P<0.01). In conclusion, miR-155 and miR-21 were highly expressed in the serum of patients, however, the recurrence and metastasis of NSCLC were even higher. The highly expressed levels of miR-155 and miR-21 were associated with the recurrence and metastasis in NSCLC patients, affecting the prognosis of patients.

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Section: Introductionmentioning

confidence: 99%

High expression of miR-155 and miR-21 in the recurrence or metastasis of non-small cell lung cancer

Shi

2019

Oncol Lett

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“…The mature miRNAs were detected and quantified by quantitative reverse‐transcription polymerase chain reaction (PCR) in conjunction with TaqMan miRNA assays (Applied Biosystems, Foster City, CA, USA) as described previously . Triplicate RNA samples were used.…”

Section: Methodsmentioning

confidence: 99%

Differential expression of microRNAs in colorectal cancer: Different patterns between isolated cancer gland and stromal cells

Sato

Fujita

Otsuka

et al. 2019

Pathology International

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Although microRNAs (miRNAs) play an important role in invasive tumor lesions, which involve cancer tissues mixed with stromal tissues, the differences in miRNA expression between cancer and stromal cells remain unclear. We selected 13 miRNAs and examined their differential expression patterns in cancer gland cells and surrounding stromal cells isolated from 24 colorectal cancer (CRC) specimens using a crypt isolation method. Although six miRNAs were upregulated in gland cells, only three were upregulated in the corresponding stromal cells, in the cancer compared with non-cancer specimens. Next, we examined the differences in miRNA expression between isolated cancer gland and stromal cells. Five miRNAs showed statistical differences in their cancer-related differential expression patterns between isolated cancer gland and stromal cells. We then compared these miRNA expression patterns in isolated cancer gland and stromal cells with those in fresh intact tumor tissues, consisting of cancer nests and stromal tissue, obtained from the 24 CRCs. The expression patterns of three miRNAs in the intact cancer tissue samples did not correspond with those in the isolated components. Identification of the expression patterns of miRNAs in both the cancer gland and stromal cell components of the tumor microenvironment greatly contributes to evaluating epigenetic regulation in CRC. K E Y W O R D Scancer-associated fibroblast, colorectal cancer, crypt isolation, microRNA, real-time PCR, stromal cell

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“…Only miRNAs with non-negative counts across all samples were retained for downstream analysis. The relative miRNA levels were indicated as median fold changes and a cutoff of two fold-change (up or down) was used 24 . A Venn diagram was prepared using FunRich 3.1.3 open source software 25 , 26 .…”

Section: Methodsmentioning

confidence: 99%

Non-coding RNAs in Various Stages of Liver Disease Leading to Hepatocellular Carcinoma: Differential Expression of miRNAs, piRNAs, lncRNAs, circRNAs, and sno/mt-RNAs

Koduru

Leberfinger

Kawasawa

et al. 2018

Sci Rep

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Hepatocellular carcinoma (HCC) was the fifth leading cause of cancer death in men and eighth leading cause of death in women in the United States in 2017. In our study, we sought to identify sncRNAs in various stages of development of HCC. We obtained publicly available small RNA-seq data derived from patients with cirrhosis (n = 14), low-grade dysplastic nodules (LGDN, n = 9), high grade dysplastic nodules (HGDN, n = 6), early hepatocellular carcinoma (eHCC, n = 6), and advanced hepatocellular carcinoma (HCC, n = 20), along with healthy liver tissue samples (n = 9). All samples were analyzed for various types of non-coding RNAs using PartekFlow software. We remapped small RNA-seq to miRBase to obtain differential expressions of miRNAs and found 87 in cirrhosis, 106 in LGDN, 59 in HGDN, 80 in eHCC, and 133 in HCC. Pathway analysis of miRNAs obtained from diseased samples compared to normal samples showed signaling pathways in the microRNA dependent EMT, CD44, and others. Additionally, we analyzed the data sets for piRNAs, lncRNAs, circRNAs, and sno/mt-RNAs. We validated the in silico data using human HCC samples with NanoString miRNA global expression. Our results suggest that publically available data is a valuable resource for sncRNA identification in HCC progression (FDR set to <0.05 for all samples) and that a data mining approach is useful for biomarker development.

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The Unknown microRNA Expression of Male Breast Cancer. Similarities and Differences with Female Ductal Carcinoma. Their Role as Tumor Biomarker

Cited by 10 publications

References 38 publications

High expression of miR-155 and miR-21 in the recurrence or metastasis of non-small cell lung cancer

High expression of miR-155 and miR-21 in the recurrence or metastasis of non-small cell lung cancer

Differential expression of microRNAs in colorectal cancer: Different patterns between isolated cancer gland and stromal cells

Non-coding RNAs in Various Stages of Liver Disease Leading to Hepatocellular Carcinoma: Differential Expression of miRNAs, piRNAs, lncRNAs, circRNAs, and sno/mt-RNAs

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