The Uptake of Manganese Dipyridoxal-Diphosphate by Chemically Induced Hepatocellular Carcinoma in Rats; A Correlation between Contrast-Media-Enhanced Magnetic Resonance Imaging, Tumor Differentiation, and Vascularization

Ni, Yicheng; Marchal, G; Zhang, X; P, Van Hecke; Michiels, Johan; J, Yu; Rummeny, EJ; Lodemann, Kp; Al, Baert

doi:10.1097/00004424-199306000-00011

Cited by 39 publications

(37 citation statements)

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“…Such technical problems can be tackled by following these two approaches [6,[9][10][11][12][13][14][15][16][17][18][19][20][31][32][33][34][35][36][37].…”

Section: Methods For Colocalizing Tumor Between In Vivo Image and Posmentioning

confidence: 99%

“…Eventually the tissue block is processed with paraffin sections and stainings such as hematoxylin-eosin (HE) or immunohistochemistry to correlate tumoral histomorphological characteristics with in vivo imaging findings. Cross-referencing the postmortem topographic, vascular and histological features of individual lesions as well as their in vivo imaging behaviors on different acquisition conditions without and with CE, up to now this technique has always allowed the longstanding experimental conclusions [9][10][11][12][13][14][15][16][17][18][19]. 2) Gross frozen-slicing approach: At the end of imaging studies, the rat is euthanized by intravenous overdose of pentobarbital.…”

Section: Methods For Colocalizing Tumor Between In Vivo Image and Posmentioning

confidence: 99%

“…It was not until early 1990s that DENA-induced HCC lesions could have been noninvasively characterized by MRI in living rats, on which a methodology paper [6] was granted the Stauffer Award in 1993 by the Association of University Radiologists and Society of Chairs of Academic Radiology Departments [http://www.aur.org/Awards_Training_Grants/stauffer.cfm]. Since then, a research platform based on this model has played a major role in preclinical evaluations of a number of newly developed specific MRI CAs [6,[9][10][11][12][13][14][15] and contributed to the discovery of necrosis avid contrast agents (NACAs) for, among many other applications, determination of myocardial infarction and anticancer therapeutic assessment [16][17][18][19][20].…”

Section: Primary Liver Cancer Model In Ratsmentioning

confidence: 99%

“…Consequently, such a comprehensive modeling system allowed appreciation of the contributions of not only intra-and extra-vascular compartments [22] but also active cellular uptake to the final contrast enhancement (CE) patterns. The latter aspect proves particularly important in the studies on tissue specific MRI CAs [6,[9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Primary Liver Cancer Model In Ratsmentioning

confidence: 99%

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Tumor models and specific contrast agents for small animal imaging in oncology

Chen

et al. 2009

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a b s t r a c tDespite the widespread use of various imaging modalities in clinical and experimental oncology without or with combined application of commercially available nonspecific contrast agents (CAs), development of tissue-or organ-or disease-specific CAs has been a continuing effort for pursuing ever-improved sensitivity, specificity, and applicability. This is particularly true with magnetic resonance imaging (MRI) due to its intrinsic superb spatial/temporal/contrast resolutions and adequate detectability for tiny amount of substances. In this context, research using small animal tumor models has played an indispensible role in preclinical exploration of tissue specific CAs. Emphasizing more on methodological and practical aspects, this article aims to share our cumulated experiences on how to create tumor models for evaluation and development of new tissue specific MRI CAs and how to apply such models in imaging-based research studies. With the results that are repeatedly confirmed by later clinical applications in cancer patients, some of our early preclinical studies have contributed to the designs of subsequent clinical trials on the new CAs, some studies have predicted new utilities of these CAs; and other studies have led to the discoveries of new tissue-or disease-specific CAs with novel diagnostic or even therapeutic potentials. Among commonly adopted tumor models, the chemically induced and surgically implanted nodules in the liver prove very useful to simulate primary and metastatic intrahepatic tumors, respectively in clinical patients. The methods to create tumor models have eased procedures and yielded high success rates. The specific properties of the new CAs could be outshined by intraindividual comparison to the commercial CAs as nonspecific controls. Meticulous imaging-microangiography-histology matching techniques guaranteed colocalization of the lesion on in vivo MRI and postmortem tissue specimen, hence correct imaging interpretation and longstanding conclusions. As exemplified in the real study cases, the present experimental set-up proves applicable in small animals for imaging-based oncological investigations, and may provide a platform for the currently booming molecular imaging in a multimodality environment.

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“…Such technical problems can be tackled by following these two approaches [6,[9][10][11][12][13][14][15][16][17][18][19][20][31][32][33][34][35][36][37].…”

Section: Methods For Colocalizing Tumor Between In Vivo Image and Posmentioning

confidence: 99%

Section: Methods For Colocalizing Tumor Between In Vivo Image and Posmentioning

confidence: 99%

Section: Primary Liver Cancer Model In Ratsmentioning

confidence: 99%

Section: Primary Liver Cancer Model In Ratsmentioning

confidence: 99%

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Tumor models and specific contrast agents for small animal imaging in oncology

Chen

et al. 2009

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“…9,10 In rats, hepatic SI enhancement after Mn-DPDP injection is reduced by a chronic (up to 5.5 months) ethanol diet 23 and in undifferentiated hepatocellular carcinoma. 24 In patients, cirrhosis also is associated with a low SI enhancement and the Child-Pugh index and the serum aspartate aminotransferase concentrations are related to the enhancement. 25,26 The enhancement is low in areas with fibrous tissues and higher in areas with regenerating nodules.…”

Section: Gd-bopta Entry Into Hepatocytes During Cirrhosismentioning

confidence: 99%

Magnetic Resonance Imaging With Hepatospecific Contrast Agents in Cirrhotic Rat Livers

Planchamp¹,

Montet²,

Frossard³

et al. 2005

Investigative Radiology

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Objective: During biliary cirrhosis in rats, organic anion-transporting peptides (Oatps) and ATP-dependent multidrug resistance-associated protein 2 (Mrp2) that are likely to transport the contrast agent Gd-BOPTA through hepatocytes are down-regulated. However, the consequences of such down-regulation on the signal intensity (SI) enhancement are unknown. Consequently, the aim of our study was to measure the hepatic SI enhancement during Gd-BOPTA perfusion as well as the Oatp and Mrp2 expression in normal and cirrhotic livers. Materials and Methods:The hepatic SI enhancement during Gd-BOPTA perfusion was measured in livers isolated from normal rats and rats that had a bile duct ligation (BDL) 15, 30, and 60 days before the perfusion. Hepatic injury and transporter expression were measured in control and cirrhotic rats. Results: BDL induced a severe hepatic injury that increased over time with a down-regulation of the transporter expression. The extracellular space (assessed by Gd-DTPA perfusion) increased with the severity of the disease. Gd-BOPTA-induced SI enhancement remained similar in BDL-15 and BDL-30 rats than in control rats but significantly decreased in severe cirrhosis (BDL-60 rats). In comparison, the Mn-DPDP-induced SI enhancement decreases proportionally to the severity of the disease. Conclusion: During biliary cirrhosis, Gd-BOPTA-induced SI enhancement could not be related to the hepatic expression of transporters.

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