“…The procedure was slightly modified by using 0.1 per cent NH40H for dilution instead of water in order to obtain more complete cytolysis (Barer and Gaffney 1957) and by using a higher degree.of dilution, which better .suited the instrument used. Calibration curves were made in the presence of the corresponding concentrations of protein precipitant used.…”
“…The procedure was slightly modified by using 0.1 per cent NH40H for dilution instead of water in order to obtain more complete cytolysis (Barer and Gaffney 1957) and by using a higher degree.of dilution, which better .suited the instrument used. Calibration curves were made in the presence of the corresponding concentrations of protein precipitant used.…”
“…Determination 0/ Na: and K+ Determination of Na" and K+ in erythrocytes was made with an "Eppendorf" flamephotometer (Netheler and Hinz GMBH, Hamburg) after hemolysis and precipitation of the proteins as according to Overman and Davis (1947). The procedure was slightly modified by using 0.1 per cent NH40H for dilution instead of water in order to obtain more complete cytolysis (Barer and Gaffney 1957) and by using a higher degree.of dilution, which better .suited the instrument used. Calibration curves were made in the presence of the corresponding concentrations of protein precipitant used.…”
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