The use of a computerized image analysis system for photometric and morphometric measurements of bovine preimplantation embryos

Youngs, Curtis R.; Pendleton, R.J.; Rorie, R.W.; Godke, R.A.

doi:10.1016/0093-691x(87)90176-2

Cited by 6 publications

(3 citation statements)

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“…3 that failed to reach the blastocyst stage of development was stained after 144 h in culture. Immediately before staining of the early blastocysts, a photograph of each embryo was taken and subjected to image analysis (Youngs et al, 1987) on a Zeiss IBAS image analyzer. Embryo diameter, embryo area, embryo sphericity (all three exclusive of the zona pellucida), and zona pellucida thickness were measured and recorded.…”

Section: Methodsmentioning

confidence: 99%

Investigations into the control of litter size in swine: I. Comparative studies on in vitro development of Meishan and Yorkshire preimplantation embryos

Youngs

Ford

McGinnis

et al. 1993

Journal of Animal Science

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Three experiments were conducted to examine the in vitro development of preimplantation embryos from the prolific Chinese Meishan pig. Experiment 1 was conducted to assess whether Meishan embryos would develop in vitro and retain their viability, whereas Exp. 2 and 3 examined the developmental pattern of Meishan embryos. In all three experiments, Yorkshire embryos served as a contemporary comparison. Ovulation and embryo recovery rates were not different between Meishan and Yorkshire gilts. Meishan embryos cultured for 96 h were capable of establishing pregnancies. The number of cell nuclei present after 144 h of culture was lower (P < .01) for Meishan than for Yorkshire blastocysts. Meishan preimplantation embryos exhibited a slower (P < .02) in vitro rate of development from the four-cell to the compact morula stage than did Yorkshire embryos. Early blastocysts from Meishan gilts, although morphologically similar in size, contained fewer (P < .06) cells than did their counterpart Yorkshire embryos. These data demonstrate that Meishan embryos develop more slowly and contain fewer cells than do Yorkshire embryos. This differing developmental pattern of Meishan preimplantation embryos, if similar to that previously reported in miniature swine and mice, may relate to increased embryo survival.

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Section: Methodsmentioning

confidence: 99%

Investigations into the control of litter size in swine: I. Comparative studies on in vitro development of Meishan and Yorkshire preimplantation embryos

Youngs

Ford

McGinnis

et al. 1993

Journal of Animal Science

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“…As a result, several alternative methods for assessing embryo quality were investigated for their potential to be less subjective and more consistent than morphological evaluation. Some of these methods included the ability of an embryo to continue its development during in vitro culture, 5,6 dye exclusion test, 7 measurement of embryonic metabolic activity, 6 glucose uptake, 8 live-dead stains, 9 computerized image analysis, 10 fluorescein diacetate vital stain, 11,12 DAPI (4′,6′-diamidino-2-phenyl-indole) staining for dead cells, 13 and in vivo culture in rabbit oviducts. 14 …”

Section: History Of Bovine Embryo Evaluationmentioning

confidence: 99%

Evaluation ofIn Vivo‐Derived Bovine Embryos

Jahnke

West

Youngs

2014

Bovine Reproduction

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“…The utilization of image analysis allows morphometric and densitometric measurements to be made from microscopic specimens. This technique has proven useful in preliminary studies of equine , caprine and bovine (Youngs et al, 1987;Casey et al, 1989Casey et al, , 1990 pre-implantation embryos. Results have indicated that changes in embryo diameter, sphericity and optical density are characteristic of preimplantation farm animal development and can be used to classify prehatching embryos according to embryo quality (Casey et al, 1990).…”

Section: Introductionmentioning

confidence: 96%

Fertilization and early embryology: Use of videocinematography to assess morphological qualities of conventionally cultured and cocultured embryos

et al. 1995

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Videocinematography and image analysis procedures were utilized to evaluate the effect of conventional and coculture methodologies on morphological parameters in human embryos derived from in-vitro fertilization (IVF). Following 24-30 h of in-vitro development, cocultured embryos had more acceptable morphological features and less fragmentation present than embryos cultured in medium alone. Cocultured embryos were more advanced at the time of replacement when compared with conventionally cultured embryos. Zona pellucida variation (> or = 20%) also occurred more frequently in cocultured embryos. The morphological characteristic most enhanced after coculture was blastomere expansion. Patients who became pregnant across both culture treatments had a higher proportion of morphologically normal embryos replaced than patients who failed to achieve an ongoing pregnancy. Clinical pregnancy rate for patients following coculture was 49%, which was greater (P < 0.05) than the 29% detected for patients with embryos in the conventional culture group.

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The use of a computerized image analysis system for photometric and morphometric measurements of bovine preimplantation embryos

Cited by 6 publications

References 0 publications

Investigations into the control of litter size in swine: I. Comparative studies on in vitro development of Meishan and Yorkshire preimplantation embryos

Investigations into the control of litter size in swine: I. Comparative studies on in vitro development of Meishan and Yorkshire preimplantation embryos

Evaluation ofIn Vivo‐Derived Bovine Embryos

Fertilization and early embryology: Use of videocinematography to assess morphological qualities of conventionally cultured and cocultured embryos

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