The Use of a Neutralizing Monoclonal Antibody to Detect Infections of Equine Herpesvirus Type 2 (EHV-2)

Nordengrahn, Ann; Klingeborn, B.; Lindholm, Å.; Mérza, M.

doi:10.1177/104063870101300504

Cited by 9 publications

(11 citation statements)

References 24 publications

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“…The EHV-2 infection of the Swedish horses was also confirmed by a bELISA [15] which showed that in stable I, 76% of the horses had detectable antibodies while only 28% of the tested animals in stable II were positive (note that stable I had annual respiratory problems). In the above mentioned study, the results indicate that the bELISA is a helpful tool to detect newly acquired EHV-2 infections since the figures agree with the clinical picture of the two stables and since it is well known that antibody levels obtained after a viral infection usually decrease with time.…”

Section: Discussionmentioning

confidence: 96%

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Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Nordengrahn¹,

Mérza²,

Ros

et al. 2002

Vet. Res.

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-Equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) have a rather unclear pathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68% in Sweden and 71% in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93%) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24% in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases. Chez les chevaux adultes, la prévalence de EHV-2 dans les LSP atteignait 68 % en Suède et 71 % au Royaume-Uni. L'ADN de EHV-2 a été détecté dans les LSP de tous les poulains testés, et la plupart (93 %) des prélèvements nasaux étaient également positifs. La préva-lence de l'ADN de EHV-5 dans les LSP des poulains en Hongrie était de 15 % et de 24 % chez les chevaux adultes au Royaume-Uni. Cette observation fait partie des très rares signalements de la présence de EHV-5 chez les chevaux. En résumé, les tests PCR spécifiques ont révélé des données importantes sur la présence et la distribution de EHV-2 et EHV-5 dans d'importantes populations de chevaux. Les résultats ont montré que l'infection par le EHV-5 se produisait plus tard que celle par le EHV-2 chez le poulain. Cette étude apporte une meilleure compréhension du rôle étiologique de ces herpesvirus gamma dans les maladies équines.infections équines / Gammaherpesvirinae / EHV-2 / EHV-5 / PCR

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Section: Discussionmentioning

confidence: 96%

“…A Hungarian isolate of EHV-2, strain KT-5797 [14] and an Australian isolate of EHV-5, strain 2-141 [8] were propagated in the rabbit kidney cell line (RK-13) as described elsewhere [15].…”

Section: Viruses and Cellsmentioning

confidence: 99%

Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Nordengrahn¹,

Mérza²,

Ros

et al. 2002

Vet. Res.

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“…This assay was performed on 96 wells RK-13 cell monolayer, where serial four-fold dilutions of complement-inactivated serum were incubated with 100 ID 50 of LK strain for 4 hs at 37°C as described elsewhere [ 25 ]. After 7 incubation days, antibody titres were calculated by Reed and Muench formula [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Equine herpesvirus 2 (EHV-2) infection in thoroughbred horses in Argentina

2005

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Background: Equine herpesvirus 2 is a gamma-herpesvirus that infects horses worldwide. Although EHV-2 has been implicated in immunosuppression in foals, upper respiratory tract disease, conjunctivitis, general malaise and poor performance, its precise role as a pathogen remains uncertain. The purpose of the present study was to analyse the incidence of EHV-2 in an Argentinean horse population and correlate it with age and clinical status of the animals.

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“…Early peri-partum infection with EHV-2 occurs in foals, 7 , 18 , 62 , 88 – 92 even though these animals generally have high titers of maternally-derived antibodies. 93 EHV-2 is most frequently transmitted horizontally to a newborn foal from its dam via the nasopharyngeal route or subsequently, through contact with other foals.…”

Section: Ehv-2 Infectionmentioning

confidence: 99%

“… 7 The prevalence of infection seems to decline at 9 months, 90 at a time when antibody levels increase. 60 , 91 , 92 Periodic reactivation of latent infections can occur throughout the life of the horse, resulting in spread of infection within a population. However, infections in later life appear mostly to be asymptomatic.…”

Section: Ehv-2 Infectionmentioning

confidence: 99%

Gammaherpesvirus infections in equids: a review

Marenzoni¹,

Stefanetti²,

Danzetta³

et al. 2015

VMRR

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Although the first equine gammaherpesvirus was identified over 50 years ago, the isolation and characterization of other members of this virus group has been relatively recent. Even so, numerous clinical syndromes have been identified in equid species in association with these viruses. Equid gammaherpesviruses are a genetically heterogeneous viral subfamily, the function of which in host immune modulation and disease pathogenesis has not yet been elucidated. While they share similarities with gammaherpesviruses in humans, the role they play in their relationship with the host is the subject of continued interest and research. Their widespread presence in horses and other equid species provides a considerable challenge in linking them with particular clinical and pathological conditions and in defining their significance from a diagnostic and therapeutic viewpoint. The present review provides an update on the taxonomy, epidemiology, and clinical syndromes, especially respiratory, reported in association with gammaherpesvirus infection in horses, donkeys, and other equid species.

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The Use of a Neutralizing Monoclonal Antibody to Detect Infections of Equine Herpesvirus Type 2 (EHV-2)

Cited by 9 publications

References 24 publications

Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Equine herpesvirus 2 (EHV-2) infection in thoroughbred horses in Argentina

Gammaherpesvirus infections in equids: a review

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