The use of E-test for the drug susceptibility testing of Mycobacterium tuberculosis – A solution or an illusion?

Verma, J.; Rawat, Deepti; Hasan, A.R.; Capoor, Malini R.; Gupta, Kunj Bihari; Deb, Monorama; Aggarwal, Praveen; Nair, Deepthi

doi:10.4103/0255-0857.58725

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…Although E-tests could successfully rapidly determination of drug resistance to streptomycin (STM), INH, RIF, and ethambutol (EMB), it existed many limitations, such as the high risk of cross-contamination, laboratory infection, expense, high false-positive rate. 47 …”

Section: Phenotypic Drug Sensitivity Testmentioning

confidence: 99%

“…Drug Resistance 2024:17 https://doi.org/10.2147/IDR.S457308 DovePress 1495 laboratory examination method for MTB includes an E-test, nitrate reductase assay, bacterial culture method, and molecular diagnostic technique, with different features and limitations. Although E-tests could successfully rapidly determination of drug resistance to streptomycin (STM), INH, RIF, and ethambutol (EMB), it existed many limitations, such as the high risk of cross-contamination, laboratory infection, expense, high false-positive rate 47. …”

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Identification of Mycobacterium tuberculosis Resistance to Common Antibiotics: An Overview of Current Methods and Techniques

Xiong,

Zhang,

Yan

et al. 2024

IDR

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Multidrug-resistant tuberculosis (MDR-TB) is an essential cause of tuberculosis treatment failure and death of tuberculosis patients. The rapid and reliable profiling of Mycobacterium tuberculosis (MTB) drug resistance in the early stage is a critical research area for public health. Then, most traditional approaches for detecting MTB are time-consuming and costly, leading to the inappropriate therapeutic schedule resting on the ambiguous information of MTB drug resistance, increasing patient economic burden, morbidity, and mortality. Therefore, novel diagnosis methods are frequently required to meet the emerging challenges of MTB drug resistance distinguish. Considering the difficulty in treating MDR-TB, it is urgently required for the development of rapid and accurate methods in the identification of drug resistance profiles of MTB in clinical diagnosis. This review discussed recent advances in MTB drug resistance detection, focusing on developing emerging approaches and their applications in tangled clinical situations. In particular, a brief overview of antibiotic resistance to MTB was present, referred to as intrinsic bacterial resistance, consisting of cell wall barriers and efflux pumping action and acquired resistance caused by genetic mutations. Then, different drug susceptibility test (DST) methods were described, including phenotype DST, genotype DST and novel DST methods. The phenotype DST includes nitrate reductase assay, Roche TM solid ratio method, and liquid culture method and genotype DST includes fluorescent PCR, GeneXpert, PCR reverse dot hybridization, ddPCR, next-generation sequencing and gene chips. Then, novel DST methods were described, including metabolism testing, cell-free DNA probe, CRISPR assay, and spectral analysis technique. The limitations, challenges, and perspectives of different techniques for drug resistance are also discussed. These methods significantly improve the detection sensitivity and accuracy of multidrug-resistant tuberculosis (MRT) and can effectively curb the incidence of drug-resistant tuberculosis and accelerate the process of tuberculosis eradication.

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Section: Phenotypic Drug Sensitivity Testmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of Mycobacterium tuberculosis Resistance to Common Antibiotics: An Overview of Current Methods and Techniques

Xiong,

Zhang,

Yan

et al. 2024

IDR

View full text Add to dashboard Cite

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Drug-Resistant TB

Xiao

Tang

Wang

et al. 2017

Handbook of Global Tuberculosis Control

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Drug Resistance in Nontuberculous Mycobacteria: Mechanisms and Models

Saxena

Spaink

Forn-Cuní

2021

Biology

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The genus Mycobacteria comprises a multitude of species known to cause serious disease in humans, including Mycobacterium tuberculosis and M. leprae, the responsible agents for tuberculosis and leprosy, respectively. In addition, there is a worldwide spike in the number of infections caused by a mixed group of species such as the M. avium, M. abscessus and M. ulcerans complexes, collectively called nontuberculous mycobacteria (NTMs). The situation is forecasted to worsen because, like tuberculosis, NTMs either naturally possess or are developing high resistance against conventional antibiotics. It is, therefore, important to implement and develop models that allow us to effectively examine the fundamental questions of NTM virulence, as well as to apply them for the discovery of new and improved therapies. This literature review will focus on the known molecular mechanisms behind drug resistance in NTM and the current models that may be used to test new effective antimicrobial therapies.

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The use of E-test for the drug susceptibility testing of Mycobacterium tuberculosis – A solution or an illusion?

Cited by 4 publications

References 15 publications

Identification of Mycobacterium tuberculosis Resistance to Common Antibiotics: An Overview of Current Methods and Techniques

Identification of Mycobacterium tuberculosis Resistance to Common Antibiotics: An Overview of Current Methods and Techniques

Drug-Resistant TB

Drug Resistance in Nontuberculous Mycobacteria: Mechanisms and Models

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