The use of glycine as nitrogen source by Escherichia coli K12

Newman, E. B.; Batist, Gerald; Fraser, J.; Isenberg, Sandra; Weyman, Philip D.; Kapoor, Vishal

doi:10.1016/0304-4165(76)90173-2

Cited by 20 publications

(15 citation statements)

References 22 publications

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“…In fact Roberts et al (1955) reported that 25-30% of the carbon of aspartate, lysine, glutamate, arginine, and proline was derived from L-serine. We similarly reported labelling of alanine and glutamate due to conversion of [14C]glycine to serine and pyruvate (Newman, Batist et al 1976).…”

Section: Discussionmentioning

confidence: 60%

An estimate of the extent of deamination of L-serine in auxotrophs of Escherichia coli K-12

Ramotar¹,

Newman²

1986

Can. J. Microbiol.

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We have shown that serine-glycine auxotrophs of Escherichia coli K-12 use exogenous L-serine inefficiently as a source of biosynthetic intermediates. Much of the L-serine supplied in the medium is not used to satisfy the auxotrophic requirement, owing to its diversion by L-serine deaminase, presumably to pyruvate. This is the first proof that the activity known as L-serine deaminase actually deaminates L-serine in vivo.

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Section: Discussionmentioning

confidence: 60%

An estimate of the extent of deamination of L-serine in auxotrophs of Escherichia coli K-12

Ramotar¹,

Newman²

1986

Can. J. Microbiol.

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“…Threonine does not serve as a nitrogen source even in strains with increased TDH levels unless the strain is also capable of using glycine as a nitrogen source (11). This supports the idea that any quantitatively important threonine catabolism proceeds via TDH to a-amino-f?-ketobutyrate (AKB) and thence to acetyl coenzyme A (CoA) and glycine via AKB ligase, a pathway which is also used in Arthrobacter spp.…”

mentioning

confidence: 56%

“…Use of threonine by an STHM-positive derivative of TE111. Strain TElll cannot use on July 16, 2020 by guest http://jb.asm.org/ Downloaded from either threonine or glycine as the source of nitrogen because it is deficient in STHM (11). We selected an STHM-positive derivative of TElll, strain TE111RR, by looking for a colony which used glycine as the nitrogen source.…”

Section: Threonine As Carbon Source 1151mentioning

confidence: 99%

Threonine as a carbon source for Escherichia coli

Chan¹,

Newman²

1981

J Bacteriol

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“…3B). This defect is likely due to a buildup of 5-CHO-THF inhibiting SHMT (6) and glycine cleavage (41), both of which E. coli needs to use glycine as nitrogen source (42). The growth defect was reversed by expressing the E. coli ygfA gene from a plasmid (Fig.…”

Section: A Candidate For 5-cho-thf Removal Predicted By Com-mentioning

confidence: 99%