The use of skin delayed‐type hypersensitivity as an adjunct test to diagnose brucellosis in cattle: A review

Bercovich, Z.

doi:10.1080/01652176.2000.9695040

Cited by 32 publications

(23 citation statements)

References 52 publications

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“…1997; Saegerman et al. 1999; Bercovich 2000). However, the advantages gained in specificity are reduced following vaccination, and the necessity for two farm visits, delay between repeat tests, and subjective nature of result interpretation, make this type of assay impractical for high throughput diagnosis.…”

Section: Diagnosticsmentioning

confidence: 99%

Brucellosis - new aspects of an old disease

Cutler¹,

Whatmore²,

Commander³

2005

Journal of Applied Microbiology

194

134

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Section: Diagnosticsmentioning

confidence: 99%

Brucellosis - new aspects of an old disease

Cutler¹,

Whatmore²,

Commander³

2005

Journal of Applied Microbiology

194

134

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“…Brucellosis is a zoonotic disease caused by the bacteria of the genus Brucella (Bercovich, 2000). Brucellosis rarely results in human death, but it can pose a significant economic loss to owners of domesticated animals due to loss of progeny, reduced milk yield, and infertility.…”

Section: Introductionmentioning

confidence: 99%

Progress in Brucella vaccine development

et al. 2012

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Brucella spp. are zoonotic, facultative intracellular pathogens, which cause animal and human disease. Animal disease results in abortion of fetuses; in humans, it manifests flu-like symptoms with an undulant fever, with osteoarthritis as a common complication of infection. Antibiotic regimens for human brucellosis patients may last several months and are not always completely effective. While there are no vaccines for humans, several licensed live Brucella vaccines are available for use in livestock. The performance of these animal vaccines is dependent upon the host species, dose, and route of immunization. Newly engineered live vaccines, lacking well-defined virulence factors, retain low residual virulence, are highly protective, and may someday replace currently used animal vaccines. These also have possible human applications. Moreover, due to their enhanced safety and efficacy in animal models, subunit vaccines for brucellosis show great promise for their application in livestock and humans. This review summarizes the progress of brucellosis vaccine development and presents an overview of candidate vaccines.

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“…abortions, though B. melitensis and B. suis might also be responsible for infection [3][4][5][6][7] . The spread of the infection is mostly via aborted fetus, fetal membranes, lochia, urine, non-pasteurized milk and milk products 8 . Brucella spp.…”

Section: Research Articlementioning

confidence: 99%

Detection of Brucella spp. in Vaginal Swab Samples of Aborting Cattle: Comparison of Immunoperoxidase to Bacteriological Culture Technique

Dağ¹,

Büyük²,

Özen³

et al. 2012

Kafkas Univ Vet Fak Derg

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Brucellosis is an important disease in terms of economic losses in cattle breeding. Brucella spp. associated abortion is common in Kars, Turkey. Determination of Brucellosis cases therefore poses great importance. In this study, an immunochemical staining technique was tested in detecting cattle with Brucellosis and compared to bacteriological culture technique. Post-abortion, total of 261 vaginal swab samples were collected from cows. Brucella spp. were isolated from 25 animals by cultural method. All Brucella isolates were identified as Brucella abortus field strain and biotyped as B. abortus biotype 3. Smears prepared from culture positive vaginal samples were stained by immunoperoxidase technique, and 22 out of 25 animals were detected as Brucella-positive. Sensitivity and specificity of the immunoperoxidase technique were calculated as 88% and 92%, respectively. Abort Yapan Sığırların Vajinal Sıvap ÖrneklerindenBrucella spp. Tespit Edilmesi: İmmunoperoksidaz ve Bakteriyolojik Kültür Yönteminin Karşılaştırılması Özet Brusellozis sığır yetiştiriciliğinde ekonomik kayıplara neden olan önemli bir hastalıktır. Kars'ta Brucella spp. nedenli abortlar oldukça yaygındır. Bu nedenle Brusellozis olgularının tespiti büyük önem arz etmektedir. Bu çalışmada; Brusellozisin tespiti amacıyla immunokimyasal boyama yöntemi test edildi ve bakteriyolojik kültür yöntemi ile karşılaştırıldı. Abortu takiben toplam 261 vajinal svap örneği ineklerden toplandı. Kültür yöntemi ile 25 hayvanda Brucella spp. izole edildi. Tüm Brucella izolatları Brucella abortus saha suşu olarak identifiye edildi ve B. abortus biyotip 3 olarak tiplendirildi. Kültür pozitif vajinal örneklerden hazırlanan sürme preparatlar immunoperoksidaz yöntemi ile boyandı ve 25 hayvandan 22'si Brucella pozitif tespit edildi. İmmunoperoksidaz yönteminin duyarlılığı ve özgüllüğü sırasıyla %88 ve %92 olarak hesaplandı. Makale Kodu (Article Code): KVFD-2012-6019Brucellosis is an important and highly contagious zoonotic disease worldwide 1,2 . It causes prominent economic losses due to abortion, stillbirth, infertility, drop in milk and meat production and loss in breeding capacity in cattle. The etiologic agent of cattle brucellosis is Brucella abortus, which is the number one cause of bacteriological DAĞ, BÜYÜK, ÖZEN, ÇELEBİ KARAMAN, AKÇA, ŞAHİN 27. Tuzcu M, Özmen M, Tuzcu N, Yoldaş A, Topçuoğlu H: Atık sığır fetüslerinde Brusellozisin patolojik, immunohistokimyasal, mikrobiyolojik yöntemlerle ve gerçek zamanlı PZR ile teşhisi. AVKAE Derg, 1, 8-14, 2011. 28. Durmaz R: Nükleik asit amplifikasyon yöntemlerinde sorunlar ve standardizasyon. In, Durmaz R (Ed): Uygulamalı Moleküler Mikrobiyoloji. s. 45-56. 2. Baskı, Nobel Tıp Kitabevleri, İstanbul, 2001. 29. Fidancı HA, Akın S, Alabay M, Güvener N: Sığırlarda Brucella abortus'a karşı oluşan antikorları saptamada ELISA ve diğer serolojik tekniklerin karşılaştırılması. Ankara Üniv Vet Fak Derg, 42, 553-557, 1995. 30. Radostits OM, Gay CC, Hinchcliff KW, Constable PD: Veterinary Medicine. 10th ed., pp. 963-984. Saunders Elsevier, ...

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The use of skin delayed‐type hypersensitivity as an adjunct test to diagnose brucellosis in cattle: A review

Cited by 32 publications

References 52 publications

Brucellosis - new aspects of an old disease

Brucellosis - new aspects of an old disease

Progress in Brucella vaccine development

Detection of Brucella spp. in Vaginal Swab Samples of Aborting Cattle: Comparison of Immunoperoxidase to Bacteriological Culture Technique

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