The use of statistical methods to evaluate winter oilseed rape doubled haploids for industrial purposes

Kaczmarek, Z.; Adamska, E.; Cegielska, T.; Szała, Laurencja

doi:10.1016/j.indcrop.2007.11.012

Cited by 7 publications

(3 citation statements)

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“…In B. napus, doubled haploid (DH) production was first described in 1975 by Thomas and Wenzel (1975). DH production is a valuable technology in modern plant breeding of oilseed rape (Cegielska-Taras et al, 2008;Kaczmarek et al, 2008;Germana, 2011). Only a small part of Brassica MDEs will regenerate to the functional DH plants; therefore, the possibility of early, concise, and relevant detection of resistant genotypes can increase the effectivity of the whole process.…”

Section: Introductionmentioning

confidence: 99%

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

et al. 2021

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The present study aims to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at the proteome level. The PEG-induced osmotic stress was studied in the cotyledonary stage of MDE of two genotypes: Cadeli (D) and Viking (V), previously reported to exhibit contrasting leaf proteome responses under drought. Two-dimensional difference gel electrophoresis (2D-DIGE) revealed 156 representative protein spots that have been selected for MALDI-TOF/TOF analysis. Sixty-three proteins have been successfully identified and divided into eight functional groups. Data are available via ProteomeXchange with identifier PXD024552. Eight selected protein accumulation trends were compared with real-time quantitative PCR (RT-qPCR). Biomass accumulation in treated D was significantly higher (3-fold) than in V, which indicates D is resistant to osmotic stress. Cultivar D displayed resistance strategy by the accumulation of proteins in energy metabolism, redox homeostasis, protein destination, and signaling functional groups, high ABA, and active cytokinins (CKs) contents. In contrast, the V protein profile displayed high requirements of energy and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick downregulation of active CKs, as well as salicylic and jasmonic acids. Genes that were suitable for gene-targeting showed significantly higher expression in treated samples and were identified as phospholipase D alpha, peroxiredoxin antioxidant, and lactoylglutathione lyase. The MDE proteome profile has been compared with the leaf proteome evaluated in our previous study. Different mechanisms to cope with osmotic stress were revealed between the genotypes studied. This proteomic study is the first step to validate MDE as a suitable model for follow-up research on the characterization of new crossings and can be used for preselection of resistant genotypes.

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Section: Introductionmentioning

confidence: 99%

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

et al. 2021

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“…Using uni-and multivariate statistical methods, DH lines were also estimated under various environmental conditions in terms of the content and stability of two different fatty acid compositions. Kaczmarek et al (2008) recommended using a model of variance analysis and related statistical methods to evaluate and select genotypes for industrial purposes. A total of 14 genotypes were found acceptable (11 stable and 3 unstable) for the chemical industry and 10 genotypes were acceptable (6 stable and 4 unstable) for the biodiesel industry.…”

Section: Genotype-environment Interactionsmentioning

confidence: 99%

Assessment of winter oilseed rape DH lines using uni- and multivariate methods of quantitative genetics and mathematical methods

Szała¹,

Kaczmarek²,

Adamska³

et al. 2015

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The development and improvement of an in vitro androgenesis of oilseed rape has allowed to obtain doubled haploid (DH) populations on a large scale. It has become possible to use DH lines in a genetic analysis of quantitative traits. Among the other benefits of DHs, homozygous state allowed to use them in a series of experiments aiming at the efficient selection of desired genotypes and their examination for adaptation in various environments. This is a review of our earlier studies which have confirmed numerous possibilities of using winter oilseed rape DH lines in a statistical analysis. Populations of DHs were used for: estimation of genetic parameters, transgression effects as well as a general and specific combining ability, genetic variation in DH populations or effects of the cross direction. In this paper, possibilities of a comprehensive multidirectional assessment of DHs lines of oilseed rape through the application of uni-and multivariate methods of quantitative genetics and mathematical methods are also presented.

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“…Therefore, FFAs in WCOs are often first esterified by homogeneous strong acid catalysts such as sulfuric or hydrochloric acid , . But liquid acids are difficult to separate from the reaction medium, which may generate wastewater and lead to the formation of a strong corrosive solution .…”

Section: Introductionmentioning

confidence: 99%

Continuous Waste Cooking Oil Transesterification with Microwave Heating and Strontium Oxide Catalyst

et al. 2017

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Biodiesel produced from waste cooking oils (WCOs) mixed with methanol was efficiently transesterified using a continuous fluid flow system with a focused microwave heating device. Strontium oxide (SrO) was added as the catalyst. The factors that most influence the biodiesel conversion rate were first estimated by considering the effects of oil-to-methanol ratio, added quantity of SrO, and microwave heating power on reaction time in a built-in batch unit. The optimal parameter values were then applied to a continuous fluid flow system, which simulates the conversion of a scaled-up quantity of WCOs into biodiesel. Under the optimum fluid flow velocity and an appropriate output temperature, a biodiesel conversion rate of ca. 93 % was reached, associated with the decomposition of ester bonds and the formation of a tetrahedral intermediate substance during the reaction.

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The use of statistical methods to evaluate winter oilseed rape doubled haploids for industrial purposes

Cited by 7 publications

References 3 publications

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

Assessment of winter oilseed rape DH lines using uni- and multivariate methods of quantitative genetics and mathematical methods

Continuous Waste Cooking Oil Transesterification with Microwave Heating and Strontium Oxide Catalyst

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