The use of virus isolation, histopathology and immunoperoxidase techniques to study the dissemination of a chicken isolate of avian pneumovirus in chickens

Catelli, Elena; Cook, Jane K.A.; Chesher, J.; Orbell, S. J.; Woods, M. A.; Baxendale, W.; Huggins, M. B.

doi:10.1080/03079459808419395

Cited by 35 publications

(48 citation statements)

References 19 publications

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“…Specific staining was shown to be closely associated with the ciliated surface epithelial cells of both experimentally infected turkeys and chickens Majó et al, 1995). Similar findings have also been reported using IP staining to study respiratory tract infections in turkeys (Majó et al, 1995) and chickens (Majó et al, 1995;Catelli et al, 1998). Interestingly, immunochemical methods have been used to demonstrate APV-specific antigen in the female reproductive tract following experimental inoculation.…”

Section: Immunochemical Assayssupporting

confidence: 63%

“…Jones et al (1988) used IF to demonstrate specific staining of the uterus of experimentally inoculated laying turkeys, while Cook et al (2000) used IP to demonstrate APV-specific staining in the oviduct of experimentally inoculated laying hens. However, the short time for which APV antigen has been detected by these methods in experimentally inoculated birds (Majó et al, 1995;Catelli et al, 1998) suggests the need for caution when using antigen detection methods to attempt to diagnose APV infection in laying birds, since a negative result may not indicate absence of infection.…”

Section: Immunochemical Assaysmentioning

confidence: 99%

“…Techniques involving the use of IP Majó et al, 1995), IF Jones et al, 1986Jones et al, , 1988 and immunogold staining (O'Loan et al, 1992) have all been reported for the detection of APV in turkeys. In addition, IF and IP (Majó et al, 1995;Catelli et al, 1998) techniques have been used successfully to detect APV in chickens.…”

Section: Immunochemical Assaysmentioning

confidence: 99%

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Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Cook¹,

Cavanagh

2002

Avian Pathology

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The available detection methods for avian pneumoviruses (turkey rhinotracheitis virus; genus Metapneumovirus) in turkeys, domestic fowl and other species are reviewed. The advantages and disadvantages of virus isolation techniques, virus or genome (polymerase chain reaction) detection and serology are discussed. Some of the problems likely to be encountered are considered, including the detection of yet to be discovered subtypes, as are the factors that are likely to influence the outcome of the work.

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Section: Immunochemical Assayssupporting

confidence: 63%

Section: Immunochemical Assaysmentioning

confidence: 99%

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Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Cook¹,

Cavanagh

2002

Avian Pathology

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“…Ethanol-fixed tissues from one jackal and one domestic dog were stained for CDV antigen at Intervet (Huntingdon, UK) in a peroxidase-anti-peroxidase reaction based on protocols described elsewhere (Catelli et al, 1998). Briefly, the tissues were dewaxed using xylene and industrial methylated spirit (IMS).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Epidemiology, Pathology, and Genetic Analysis of a Canine Distemper Epidemic in Namibia

Gowtage-Sequeira

Banyard

Barrett

et al. 2009

Journal of Wildlife Diseases

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ABSTRACT:Severe population declines have resulted from the spillover of canine distemper virus (CDV) into susceptible wildlife, with both domestic and wild canids being involved in the maintenance and transmission of the virus. This study (March 2001 to October 2003 collated case data, serologic, pathologic, and molecular data to describe the spillover of CDV from domestic dogs (Canis familiaris) to black-backed jackals (Canis mesomelas) during an epidemic on the Namibian coast. Antibody prevalence in jackals peaked at 74.1%, and the clinical signs and histopathologic observations closely resembled those observed in domestic dog cases. Viral RNA was isolated from the brain of a domestic dog from the outbreak area. Sequence data from the phosphoprotein (P) gene and the hemagglutinin (H) genes were used for phylogenetic analyses. The P gene sequence from the domestic dog shared 98% identity with the sequence data available for other CDV isolates of African carnivores. For the H gene, the two sequences available from the outbreak that decimated the lion population in Tanzania in 1994 were the closest match with the Namibian sample, being 94% identical across 1,122 base pairs (bp). Phylogenetic analyses based on this region clustered the Namibian sample with the CDV that is within the morbilliviruses. This is the first description of an epidemic involving black-backed jackals in Namibia, demonstrating that this species has the capacity for rapid and large-scale dissemination of CDV. This work highlights the threat posed to endangered wildlife in Namibia by the spillover of CDV from domestic dog populations. Very few sequence data are currently available for CDV isolates from African carnivores, and this work provides the first sequence data from a Namibian CDV isolate.

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“…Through the years, several methods that do not require a centrifuge have been proposed. [1][2][3][4][5] One of these uses a special device, consisting of several precisely assembled, machined, plastic and metal parts. 2,3 A simple cutoff standard plastic centrifuge tube has also been recommended by others.…”

Section: An Inexpensive Sedimentation Chamber For the Preparation Of mentioning

confidence: 99%

Design, Validation, and Absolute Sensitivity of a Novel Test for the Molecular Detection of Avian Pneumovirus

et al. 2004

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Abstract. This study describes attempts to increase and measure sensitivity of molecular tests to detect avian pneumovirus (APV). Polymerase chain reaction (PCR) diagnostic tests were designed for the detection of nucleic acid from an A-type APV genome. The objective was selection of PCR oligonucleotide combinations, which would provide the greatest test sensitivity and thereby enable optimal detection when used for later testing of field materials. Relative and absolute test sensitivities could be determined because of laboratory access to known quantities of purified full-length DNA copies of APV genome derived from the same A-type virus. Four new nested PCR tests were designed in the fusion (F) protein (2 tests), small hydrophobic (SH) protein (1 test), and nucleocapsid (N) protein (1 test) genes and compared with an established test in the attachment (G) protein gene. Known amounts of full-length APV genome were serially diluted 10-fold, and these dilutions were used as templates for the different tests. Sensitivities were found to differ between the tests, the most sensitive being the established G test, which proved able to detect 6,000 copies of the G gene. The G test contained predominantly pyrimidine residues at its 3Ј termini, and because of this, oligonucleotides for the most sensitive F test were modified to incorporate the same residue types at their 3Ј termini. This was found to increase sensitivity, so that after full 3Ј pyrimidine substitutions, the F test became able to detect 600 copies of the F gene.

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The use of virus isolation, histopathology and immunoperoxidase techniques to study the dissemination of a chicken isolate of avian pneumovirus in chickens

Cited by 35 publications

References 19 publications

Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Detection and differentiation of avian pneumoviruses (metapneumoviruses)

Epidemiology, Pathology, and Genetic Analysis of a Canine Distemper Epidemic in Namibia

Design, Validation, and Absolute Sensitivity of a Novel Test for the Molecular Detection of Avian Pneumovirus

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