The utility of the Sysmex XE‐2100 analyzer’s NEUT‐X and NEUT‐Y parameters for detecting neutrophil dysplasia in myelodysplastic syndromes

Abstract

The diagnosis of myelodysplastic syndromes (MDS) is based on morphological changes in the blood and bone marrow. The parameters NEUT-X and NEUT-Y of the Sysmex XE-2100 analyzer could help detect neutrophil dysplasia. A control group of 50 patients, along with 50 postpartum patients, 50 anemias, 50 leukopenias, 50 patients with microscopically visible hypergranulated neutrophils and 50 MDS patients were assessed. The NEUT-X and NEUT-Y values (mean +/- SD) for the control group were 1346 +/- 28.2 and 420 +/- 19.3, respectively, with the anemia and leukopenia groups giving similar values. The postpartum and hypergranulated neutrophils groups presented higher values (P < 0.05), whereas the values in the MDS group were 1286 +/- 72.8 and 385 +/- 50.9 (P < 0.05), respectively. There were no differences between the morphological MDS types. The NEUT-X and NEUT-Y values in MDS patients with optical hypogranulation were significantly lower than for MDS patients without optical hypogranulation. NEUT-X and NEUT-Y values lower than 1298 and 398, respectively, would have a specificity for detecting MDS of 94% and 91% and would detect 60% and 56% of cases, respectively, whereas they would detect 75% and 74%, respectively, of MDS cases with optical hypogranulation. NEUT-X and NEUT-Y parameters can be used to detect neutrophil dysplasia arising from MDS and chronic myelomonocytic leukemia.

Utility of neut-X, neut-Y and neut-Z parameters for rapidly assessing sepsis in tumor patients

“…1). neut-X is the mean value for SSC, which analyzes the inner complexity of neutrophils, and neut-Y is the mean value for fluorescence intensity, which reveals the cellular nucleic acid/protein content of neutrophils [10]. Simultaneously, we created a new parameter, neut-Z ( Fig.…”

Clinical significance of cell population data (CPD) on Sysmex XN-9000 in septic patients with our without liver impairment

“…These basically include IG (10,11), or the high fluorescence lymphocyte cells (HFLCs) count, a parameter which is usually related to the presence of activated lymphocytes, plasma cells and other blast cells (12). Additional research parameters characterizing different leukocyte populations [i.e., cell population data (CPD)] such as neutrophils (NE), lymphocytes (LY) and monocytes (MO) have recently became available, and preliminary observations suggest that their assessment may be useful in the diagnosis of myelodysplastic syndromes (13,14) and sepsis (15).…”

Contribution of the new XN‐1000 parameters NEUT‐RI and NEUT‐WY for managing patients with immature granulocytes

“…Neutrophil Granularity Intensity is the mean value of high angle diffraction and represents the complexity of the neutrophils (nucleus lobularity, granulations) and has proven to be related to dysplasia of neutrophils on XE-2100 [2,3] and to be independent of the IG% value. Combined with other parameters, it is useful for detecting MDS syndromes or chronic myelomonocytic leukaemia (CMML) at diagnosis and is an effective contribution to both laboratory clinical contribution and workflow improvement.…”