The Value of the Expression of K-Ras and Dna-Status in the Progression of Endometrioid Endometrial Carcinoma in Patients With Early Stages of Tumor Process

Iurchenko, N P; Glushchenko, N M; Skachkova, O.; Marchenko, I. O.; Buchynska, L G

doi:10.15407/oncology.2023.01.039

Cited by 4 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Laser flow cytometry was used to determine the proliferation index (PI, %, the number of cells in S+G 2 /M phases of the mitotic cycle) [18]. The study was performed on an EPICSXL flow cytometer (Beck man Coulter, USA).…”

Section: Methodsmentioning

confidence: 99%

Morphological characteristics and expression of adhesion markers in cells of low differentiated endometrial carcinoma

2023

View full text Add to dashboard Cite

Summary. The aim of the study was to evaluate the morphological features of endometrioid carcinoma of the endometrium (ECE) of low differentiation grade with different invasive potential and to characterize their molecular phenotype by the expression of a number of adhesion markers. Materials and Methods: We have studied the samples of operation material of 37 patients with ECE of low differentiation grade with deep invasion (> ½ myometrium), n = 26, and with invasion < ½ myometrium, n = 11, with the use of morphological and immunohistochemical methods, and flow cytometry. Results: In the morphological study of tumors with deep invasion in the myometrium, we have detected pronounced structural heterogeneity, which became the basis for the discretion of two groups of tumors with different characteristics of morphological phenotypes. In the majority of cases, solid layers and glandular-like structures are detected, and the similarity of the tumor epithelium with the elements of the endometrium is completely lost. In such tumors high expression of adhesion molecules – E-cadherin, CD44, CD24, and β-catenin and low expression of the marker of mesenchymal tissues — vimentin were determined. Other tumors were characterized by morphological features of the epithelial-mesenchymal transition (EMT), with the decrease of the expression of E-cadherin, β-catenin, CD24, CD44, and a significant increase in vimentin expression in comparison with these indices in tumors without signs of EMT. In ECEs that invade < ½ myometrium, the morphological indices of malignancy were less pronounced, which was associated by the changes in the expression of the molecular markers. Conclusion: This comprehensive study has established associations between the morphological heterogeneity of ECE and the expression of adhesion markers and vimentin, which is important for understanding the mechanisms of tumor cell migration.

show abstract

Section: Methodsmentioning

confidence: 99%

Morphological characteristics and expression of adhesion markers in cells of low differentiated endometrial carcinoma

2023

View full text Add to dashboard Cite

show abstract

“…The change in miRNA expression compared to the control was calculated �y the formulas of � -ΔCt . The proliferative activity of the studied endometrial carcinoma was determined �y the proliferation index �PI� %�� using the method of flow cytofluorometry [5]. The study was performed on a flow cytofluorimeter EPICS-XL �Beckman Coulter� USA�.…”

Section: Methodsmentioning

confidence: 99%

“…Today� according to the recommendations of the European Society of Medical Oncologists� ECE is divided into four molecular su�types� which are characterized �y a certain range of mutations and gene expression profiles� which require different treatment strategies [��]. In our previous studies� it was shown that aggressive ECE forms are characterized �y higher expression of cyclins E and �1� transcription factor E�F1 and aneuploidy than tumors with low potential for malignancy [5]. The association of varia�ility of the morphological ECE phenotype with changes in the expression of the markers of epithelial-mesenchymal transition �EMT� was revealed and their relation to possi�le pathways of tumor cell migration was su�stantiated [6].…”

mentioning

confidence: 96%

Expression of microRNA in tumor cells of endmetrioid carcinoma of endometrium

et al. 2023

View full text Add to dashboard Cite

Summary. Background: It is known that more than half of the genes encoding human proteins are regulated by various microRNAs (miRNAs, miR), the expression of which may be associated with various pathological conditions. At the same time, the question of assessing the relationship between the expression of particular miRNAs and the aggressive molecular subtype of endometrial cancer remains open. Aim of the study was to determine the relationship between the expression of miR-34a, miR-125b, miR-142 and miR-101 in endometrioid carcinomas of the endometrium (ECE) and the features of the disease course. Materials and Methods: The samples of surgical material of 51 patients with ECE (mean age 59.8 ± 7.1 years), I–III stage were investigated using morphological, immunohistochemical methods, real time polymerase chain reaction (PCR), cytofluorometry. Results: In endometrial tumors with high proliferation index (> Me), the expression of miR-34a, miR-142 and miR-125b significantly decreased (1.8, 2.7 and 1.5 times, respectively) compared with those in ECE with low proliferation index (< Me). The expression of all studied miRNAs was lower in G3 tumors and those that deeply invaded the myometrium compared to G2 carcinomas and tumors with an invasion of < 1/2 myometrium and significantly decreased in tumors of patients with low stage III compared with stage I–II. The high (> Me) microvessel density in ECE was associated with a significant decrease of miR-125b and miR-101 expression, and the presence of signs of epithelial-mesenchymal transition — with a decreased expression of miR-34a and miR-101. Conclusions: The study revealed a significant heterogeneity of expression of miR-34a, miR-125b, miR-142 and miR-101 in ECE, which is associated with changes in morphofunctional characteristics of endometrial carcinoma.

show abstract

“…Current model of EC pathogenesis is viewed from the position of state-of-the-art data, obtained from the studies of molecular-biological specificities of tumors. These studies helped to determine the biological heterogeneity of ECE defining the clinical course and being responsible for the differences in the aggressiveness of the tumor process [9][10][11][12][13][14][15][16].…”

mentioning

confidence: 99%

“…The existing data of scientific literature and the results of our research have demonstrated that the tumor progression in ECE is associated with unfavorable clinical course and the impaired expression of some suppressor genes, oncogenes, cellular cycle regulators, and markers of epithelial-mesenchymal transition (EMT) [6,[14][15][16]18]. However, it has not yet been clear how to discern ECE with high potential of malignancy.…”

mentioning

confidence: 99%

Molecular phenotype of high-grade endometrioid carcinoma of the endometrium

et al. 2023

View full text Add to dashboard Cite

Summary. Background: Prognosis of the course of tumor progression is one of urgent problems of clinical oncology. A relevant specificity of endometrial cancer is its clinical polymorphism within the same histological type of the disease. The search for molecular-biological features associated with the aggressive phenotype of endometrioid carcinomas is indisputably urgent. Aim: To study molecular-biological features of endometrioid carcinoma of the endometrium (ECE) and to identify the molecular subtype of tumors with high potential of malignancy. Materials and Methods: Surgical specimens of 127 patients with EC, stages I–II, aged 36–72 (the average age — 59.3 ± 3.2) were studied using morphological and immunohistochemical methods. The multivariant analysis with the Kullback’s informative measure and PanelomiX were used to estimate the significance of the expression of specific biomarkers. Results: The expression of a complex of multifunctional markers was evaluated in ECE cells of different malignancy stage: p53, FOXP3, p21WAF1/CIP1, р16INK4a, E2F1, cyclins Е and D1, Her2/neu, с-Myc, Е-cadherin, β-catenin, vimentin, CD44, CD24. A triad of biomarkers with threshold expression levels was determined (р53 > 45%; FOXP3 < 14%; с-Myc > 10%). The high expression of oncogene c-Myc and oncosuppressor p53 along with the low level of FOXP3 in tumor cells of ECE was associated with high proliferative potential, low differentiation grade, and deep invasion of a tumor into the myometrium. Conclusions: The molecular phenotype of ECE, most informative in terms of specificity and sensitivity (95%) — р53highFOXP3lowc-Mychigh, was first characterized, which would help identify a high-grade subtype of this cancer form.

show abstract

The Value of the Expression of K-Ras and Dna-Status in the Progression of Endometrioid Endometrial Carcinoma in Patients With Early Stages of Tumor Process

Cited by 4 publications

References 22 publications

Morphological characteristics and expression of adhesion markers in cells of low differentiated endometrial carcinoma

Morphological characteristics and expression of adhesion markers in cells of low differentiated endometrial carcinoma

Expression of microRNA in tumor cells of endmetrioid carcinoma of endometrium

Molecular phenotype of high-grade endometrioid carcinoma of the endometrium

Contact Info

Product

Resources

About