The VASCULATURE COMPLEXITY AND CONNECTIVITY Gene Encodes a Plant-Specific Protein Required for Embryo Provasculature Development

Roschzttardtz, Hannetz; Páez-Valencia, Julio; Dittakavi, Tejaswi; Jali, Sathya Sheela; Reyes, Francisca; Baisa, Gary; Anne, Pauline; Gissot, Lionel; Palauqui, Jean-Christophe; Masson, Patrick; Bednarek, Sebastian Y.; Otegui, Marisa S.

doi:10.1104/pp.114.246314

Cited by 24 publications

(54 citation statements)

References 65 publications

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“…S11). Furthermore, we analyzed the stomatal morphology, density, and leaf vein networks (Roschzttardtz et al, 2014) of pme34; however, there were no significant correlations with the plants lacking PME34 gene expression (Supplemental Figs. S11-S13).…”

Section: Pme34 Affects Transpiration and Stomatal Movementmentioning

confidence: 99%

PECTIN METHYLESTERASE34 Contributes to Heat Tolerance through Its Role in Promoting Stomatal Movement

Huang

Wang

et al. 2017

Plant Physiol.

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Pectin, a major component of the primary cell wall, is synthesized in the Golgi apparatus and exported to the cell wall in a highly methylesterified form, then is partially demethylesterified by pectin methylesterases (PMEs; EC 3.1.1.11). PME activity on the status of pectin methylesterification profoundly affects the properties of pectin and, thereby, is critical for plant development and the plant defense response, although the roles of PMEs under heat stress (HS) are poorly understood. Functional genome annotation predicts that at least 66 potential PME genes are contained in Arabidopsis (Arabidopsis thaliana). Thermotolerance assays of PME gene T-DNA insertion lines revealed two null mutant alleles of PME34 (At3g49220) that both consistently showed reduced thermotolerance. Nevertheless, their impairment was independently associated with the expression of HS-responsive genes. It was also observed that PME34 transcription was induced by abscisic acid and highly expressed in guard cells. We showed that the PME34 mutation has a defect in the control of stomatal movement and greatly altered PME and polygalacturonase (EC 3.2.1.15) activity, resulting in a heat-sensitive phenotype. PME34 has a role in the regulation of transpiration through the control of the stomatal aperture due to its cell wall-modifying enzyme activity during the HS response. Hence, PME34 is required for regulating guard cell wall flexibility to mediate the heat response in Arabidopsis.

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Section: Pme34 Affects Transpiration and Stomatal Movementmentioning

confidence: 99%

PECTIN METHYLESTERASE34 Contributes to Heat Tolerance through Its Role in Promoting Stomatal Movement

Huang

Wang

et al. 2017

Plant Physiol.

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“…The molecular mechanisms by which vascular tissues acquire their identities are yet largely unknown (Roschzttardtz et al, 2014), and there is high interest in analyzing and evaluating traits of veins or leaf venation networks and their genetic regulation. The impact of vein density on photosynthesis is a major investigated topic (Sack and Scoffoni, 2013).…”

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confidence: 99%

phenoVein - A tool for leaf vein segmentation and analysis

et al. 2015

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Precise measurements of leaf vein traits are an important aspect of plant phenotyping for ecological and genetic research. Here, we present a powerful and user-friendly image analysis tool named phenoVein. It is dedicated to automated segmenting and analyzing of leaf veins in images acquired with different imaging modalities (microscope, macrophotography, etc.), including options for comfortable manual correction. Advanced image filtering emphasizes veins from the background and compensates for local brightness inhomogeneities. The most important traits being calculated are total vein length, vein density, piecewise vein lengths and widths, areole area, and skeleton graph statistics, like the number of branching or ending points. For the determination of vein widths, a model-based vein edge estimation approach has been implemented. Validation was performed for the measurement of vein length, vein width, and vein density of Arabidopsis (Arabidopsis thaliana), proving the reliability of phenoVein. We demonstrate the power of phenoVein on a set of previously described vein structure mutants of Arabidopsis (hemivenata, ondulata3, and asymmetric leaves2-101) compared with wild-type accessions Columbia-0 and Landsberg erecta-0. phenoVein is freely available as open-source software.

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“…VCC has previously been reported to be expressed in different plant organs (Roschzttardtz et al ., ). Our results suggest that VCC is required for leaf patterning in the early stages of leaf primordium development.…”

Section: Resultsmentioning

confidence: 97%

Members of the DEAL subfamily of the DUF1218 gene family are required for bilateral symmetry but not for dorsoventrality in Arabidopsis leaves

et al. 2017

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Most plant leaves exhibit bilateral symmetry, which has been hypothesized as an inevitable consequence of the existence of the proximodistal and dorsoventral axes. No gene has been described that affects leaf bilateral symmetry but not dorsoventrality in Arabidopsis thaliana. We screened for viable insertional mutations that affect leaf morphology, and out of more than 700 mutants found only one, desigual1-1 (deal1-1), that exhibited bilateral symmetry breaking but no obvious defects in dorsoventrality. We found that deal1-1 is an allele of VASCULATURE COMPLEXITY AND CONNECTIVITY (VCC). Several overlapping regulatory pathways establish the interspersed lobes and indentations along the margin of Arabidopsis thaliana leaves. These pathways involve feedback loops of auxin, the PIN-FORMED1 (PIN1) auxin efflux carrier, and the CUP-SHAPED COTYLEDON2 (CUC2) transcriptional regulator. Early vcc (deal1) leaf primordia fail to acquire bilateral symmetry and instead form ectopic lobes and sinuses. The vcc leaves show aberrant recruitment of marginal cells expressing properly polarized PIN1, resulting in misplaced auxin maxima. Normal PIN1 polarization requires CUC2 expression and CUC2 genetically interacts with VCC; VCC also affects CUC2 expression. VCC has a domain of unknown function, DUF1218, and localizes to the endoplasmic reticulum membrane. VCC acts partially redundantly with its two closest paralogs, DEAL2 and DEAL3, in early leaf margin patterning and is required for bilateral symmetry, but its loss of function does not visibly affect dorsoventrality.

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The VASCULATURE COMPLEXITY AND CONNECTIVITY Gene Encodes a Plant-Specific Protein Required for Embryo Provasculature Development

Cited by 24 publications

References 65 publications

PECTIN METHYLESTERASE34 Contributes to Heat Tolerance through Its Role in Promoting Stomatal Movement

PECTIN METHYLESTERASE34 Contributes to Heat Tolerance through Its Role in Promoting Stomatal Movement

phenoVein - A tool for leaf vein segmentation and analysis

Members of the DEAL subfamily of the DUF1218 gene family are required for bilateral symmetry but not for dorsoventrality in Arabidopsis leaves

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