The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

Matny, Oadi; Matny, Oadi; Gourdoupis, Spyridon; Johnson, Ryan C.; Aljedaani, Fatimah R.; Blilou, Ikram; Gardener, Catherine H.; Yue, Yajuan; Kangara, Ngonidzashe; Steuernagel, Burkhard; Hayta, Şadiye; Smedley, Mark; Harwood, Wendy; Patpour, Mehran; Wu, Shuangye; Poland, Jesse; Jones, Jonathan D. G.; Reuber, T. Lynne; Ronen, Moshe; Sharon, Amir; Rouse, Matthew N.; Xu, Steven S.; Holušová, Kateřina; Bartoš, Jan; Molnár, István; Karafiátová, Miroslava; Jaremko, Łukasz; Doležel, Jaroslav; Steffenson, Brian J.; Wulff, Brande B. H.

doi:10.21203/rs.3.rs-1820134/v1

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…Further, oat lines with multiple sources of resistance may begin segregating following their initial release, further complicating the interpretation of phenotyping conducted at different institutions (Nguyen et al 2023). Finally, resistance loci effective against other rust fungi display temperature sensitivity (Adhikari et al 2000;Gousseau et al 1985;Niu et al 2014;Yu et al 2023).…”

Section: Discussionmentioning

confidence: 99%

Detection of oat crown rust disease in Taiwan (2019-2021)

Ho,

Henningsen,

Chen

et al. 2024

Preprint

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Oat is a minor forage crop grown in Taiwan. Only a few historical records of oat rust disease have been reported in the country, therefore the pathogen population remains poorly characterized. A rust-like disease outbreak was detected at the Experimental Farm of National Taiwan University in 2019, which caused significant damage to the field experiments. To determine the identity of the pathogen responsible for this disease outbreak, we collected infected foliar material. Disease signs suggested infection by the oat crown rust fungus. Hence, common procedures in rust pathology were applied to confirm the identity of the pathogen with phenotypic and molecular diagnostic techniques. A total of 50 field samples from infected oat cultivars were collected in 2019 and five rust isolates were purified in 2020 and 2021. Phylogenetic analysis based on ITS sequences indicated that the pathogen was likely Puccinia coronata f. sp. avenae (Pca), which was further supported by the placement of Taiwanese isolate NTU-01 with other Pca representatives in a phylogenetic tree of Basidiomycete fungi. Phenotyping assays across 36 oat differential lines demonstrated that Taiwanese isolates are phenotypically similar with relatively limited virulence. This study presents the first molecular confirmation of Pca in Taiwan and reports the virulence profiles of Taiwanese Pca population.

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Section: Discussionmentioning

confidence: 99%

Detection of oat crown rust disease in Taiwan (2019-2021)

Ho,

Henningsen,

Chen

et al. 2024

Preprint

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“…The 2022 field season coincided with a record high heat dome across the Pacific Northwest. Many stem rust resistance genes in wheat are known to be temperature sensitive, such as Sr6 (Leonard & Szabo, 2005), Sr15 (Gao et al., 2019), Sr43 (Yu et al., 2023). Lines carrying these genes usually exhibit higher infection types at elevated temperatures.…”

Section: Characteristicsmentioning

confidence: 99%

Registration of the “Woodies” multi–rust‐resistant barley germplasm

Massman,

Hernandez,

Clare

et al. 2024

J of Plant Registrations

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Selection for resistance to plant diseases is a continuous effort on the part of plant breeders. Sources of genetic resistance are often limited, despite considerable discovery efforts. Stem rust and stripe rust are two diseases of particular importance in barley (Hordeum vulgare L.) production. The present work aims to develop and deploy genotypes with resistance to these diseases that can be used in future breeding efforts. The Woodies, Woody‐1 (DH160733; Reg. no. GP‐218, PI 704479) and Woody‐2 (DH160754; Reg. no. GP‐219, PI 704480), are two doubled‐haploid genotypes produced via F1 anther culture named in honor of the late Lynn “Woody” Gallagher. These two‐row spring habit barley germplasm accessions were released by the Oregon Agricultural Experiment Station in 2023. These genotypes have demonstrated resistance to both stem and stripe rust at the seedling and adult plant stage in trials conducted between 2018 and 2023. The genetic basis of this resistance appears to be a novel quantitative trait locus conferring resistance to both diseases on chromosome 5H that is different from the known rpg4/Rpg5 complex for stem rust resistance found on the same chromosome. Seed can be requested from the Oregon State University Barley Breeding Program or from the NLGRP Germplasm repository.

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“…Morex delimited the candidate locus to two possible genes (Dinh et al ., 2022). Similarly, cloning and characterization of R genes encoding kinase fusion proteins, such as Resistance to Puccinia graminis 1 ( Rpg1 ) (Brueggeman et al ., 2002), Yellow rust resistance 15 ( Yr15 ) (Klymiuk et al ., 2015), Lr9 (Wang et al ., 2023), Stem rust resistance 43 ( Sr43 ) (Yu et al ., 2023), Sr60 (Chen et al ., 2020), Sr62 (Yu et al ., 2022), WTK4 (Gaurav et al ., 2022), Powdery mildew resistance 4 ( Pm4 ) (Sánchez‐Martín et al ., 2021), Pm24 (Lu et al ., 2020), Pm13 (Li et al ., 2023), Pm57 (Liu et al ., 2023) and Rwt4 (synonym of Resistance to Magnaporthe grisea 1 ; Rmg1 ) (Arora et al ., 2023), along with the Kolodziej et al . (2021) and Dinh et al .…”

Section: Pan‐genomics Reveal the Extent Of Variation At R Locimentioning

confidence: 99%

“…Morex delimited the candidate locus to two possible genes (Dinh et al, 2022). Similarly, cloning and characterization of R genes encoding kinase fusion proteins, such as Resistance to Puccinia graminis 1 (Rpg1) (Brueggeman et al, 2002), Yellow rust resistance 15 (Yr15) (Klymiuk et al, 2015), Lr9 (Wang et al, 2023), Stem rust resistance 43 (Sr43) (Yu et al, 2023) Dinh et al (2022) studies suggest that as many as 35% of major, dominant, racespecific R genes to biotrophic pathogens of wheat and barley do not encode canonical NLR immune receptors (Table S3). Many of these (18 out of 85, 20%) encode protein kinase proteins containing fusion domains.…”

Section: Capturing Functional Diversity Through Genomics-enhanced Ass...mentioning

confidence: 99%

Resistance that stacks up: engineering rust and mildew disease control in the cereal crops wheat and barley

Dracatos

Sánchez‐Martín

et al. 2023

Plant Biotechnology Journal

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SummaryStaying ahead of the arms race against rust and mildew diseases in cereal crops is essential to maintain and preserve food security. The methodological challenges associated with conventional resistance breeding are major bottlenecks for deploying resistance (R) genes in high‐yielding crop varieties. Advancements in our knowledge of plant genomes, structural mechanisms, innovations in bioinformatics, and improved plant transformation techniques have alleviated this bottleneck by permitting rapid gene isolation, functional studies, directed engineering of synthetic resistance and precise genome manipulation in elite crop cultivars. Most cloned cereal R genes encode canonical immune receptors which, on their own, are prone to being overcome through selection for resistance‐evading pathogenic strains. However, the increasingly large repertoire of cloned R genes permits multi‐gene stacking that, in principle, should provide longer‐lasting resistance. This review discusses how these genomics‐enabled developments are leading to new breeding and biotechnological opportunities to achieve durable rust and powdery mildew control in cereals.

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The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

Cited by 8 publications

References 31 publications

Detection of oat crown rust disease in Taiwan (2019-2021)

Detection of oat crown rust disease in Taiwan (2019-2021)

Registration of the “Woodies” multi–rust‐resistant barley germplasm

Resistance that stacks up: engineering rust and mildew disease control in the cereal crops wheat and barley

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