The whole chloroplast genome sequence of Macadamia tetraphylla (Proteaceae)

Mitochondrial DNA Part B

Liu

2019

Self Cite

Helicia nilagirica Bedd. is a medicinal plant. Its fruit is the main raw material from which helicid, a compound that has sedative, hypnotic, analgesic, and other central nervous system inhibitory effects, is extracted. Here, we report and characterize the complete chloroplast genome of H. nilagirica in this study. The complete chloroplast genome of H. nilagirica contains 157,217 bp and consists of four regions, including a large single-copy region (LSC, 85,516 bp), two inverted repeat regions (IRs, 26,700 bp), and a small single copy (SSC, 18,301 bp) region. A total of 132 genes were obtained by annotation, including 88 protein-coding genes, 36 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. Phylogenetic analysis showed that H. nilagirica is closely related to Macadamia integrifolia, suggesting that H. nilagirica may be used as rootstock or gene donor in macadamia breeding. This study lays a foundation for future phylogenetic and evolutionary studies of Proteaceae.

Section: Helicia Nilagirica Bedd; Chloroplast Genome; Phylogenetic Amentioning

confidence: 99%

Complete chloroplast genome of Helicia nilagirica Bedd. and its phylogenetic analysis

Mitochondrial DNA Part B

Liu

2019

Self Cite

“…Mb to 780 Mb [17][18][19] , but research on the genome of macadamia was very few. In 2014, 2017 and 2018, the chloroplast genome of M. integrifolia, M. ternifolia and M. tetraphylla have been sequenced by Australian and Chinese researchers [20][21][22] , and in 2016, the darft genome and transcriptome of M. integrifolia cultivar 741 have been sequenced, a total length of 518 Mb have been assembly, which spans approximately 79% of the 3 estimated genome size 18 . But up to now, the genome sequencing of M. tetraphylla has not yet been reported.…”

mentioning

confidence: 99%

Genome assembly and annotation ofMacadamia tetraphylla

et al. 2020

Preprint

Self Cite

Macadamia is a kind of evergreen nut trees which belong to the Proteaceae family. The two commercial macadamia species, Macadamia integrifolia and M. tetraphylla, are highly prized for their edible kernels. Catherine et al. reported M. integrifolia genome using NGS sequencing technology. However, the lack of a high-quality assembly for M. tetraphylla hinders the progress in biological research and breeding program. In this study, we report a high-quality genome sequence of M. tetraphylla using the Oxford Nanopore Technologies (ONT) technology. We generated an assembly of 750.54 Mb with a contig N50 length of 1.18 Mb, which is close to the size estimated by flow cytometry and k-mer analysis. Repetitive sequence represent 58.57% of the genome sequence, which is strikingly higher compared with M. integrifolia. A total of 31,571 protein-coding genes were annotated with an average length of 6,055 bp, of which 92.59% were functionally annotated. The genome sequence of M. tetraphylla will provide novel insights into the breeding of novel strains and genetic improvement of agronomic traits.

“…Sequencing information of the chloroplast is important for genetic improvement and toward an understanding of biological mechanisms of the plants (Shearman et al 2014). Furthermore, the chloroplast sequences have often been used to study phylogenetic relationships between plants (Tangphatsornruang et al 2010;Liu et al 2018).…”

mentioning

confidence: 99%

The complete chloroplast genome of Hevea camargoana

Mitochondrial DNA Part B

Zheng

et al. 2020

Self Cite

Hevea camargoana is a natural latex producing tropical plant and a close relative of H. brasiliensis, the primary commercial source of natural rubber. This study sequenced and analyzed the chloroplast genome of H. camargoana. The circular chloroplast genome of H. camargoana contains 161,291 bp with a GC content of 35.72%. This region contains two inverted repeat regions (26,819 bp), a large single-copy region (89,281 bp), and a small single-copy (18,372 bp) region in the complete chloroplast genome. A total of 134 genes were annotated, including 86 protein-coding genes, 36 transfer RNA genes, 8 ribosomal RNA genes, and 4 pseudogenes. The results showed that H. camargoana and H. brasiliensis were closely related, suggesting that H. camargoana may be used for the future variety improvement of rubber trees.