The WNT Signalling Modulator, Wise, is Expressed in an Interaction-Dependent Manner During Hair-Follicle Cycling

O'Shaughnessy, R; Yeo, Weeteck; Gautier, Jean; Jahoda, Colin A.B.; Christiano, Angela M.

doi:10.1111/j.0022-202x.2004.23410.x

Cited by 32 publications

(23 citation statements)

References 40 publications

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“…Based on our observations and the known sites of epidermal Wnt expression and activity (DasGupta and Fuchs, 1999;Reddy et al, 2001), we propose that in the hair follicle pre-cortex ␤-catenin/Lef1 activity induces Jag1 expression, which then activates Notch in the pre-cortex and underlying dermal papilla cells (O'Shaughnessy et al, 2004;Rendl et al, 2005) to promote differentiation of hair follicle lineages (Yamamoto et al, 2003).…”

Section: Research Articlementioning

confidence: 60%

Jagged 1 is a β-catenin target gene required for ectopic hair follicle formation in adult epidermis

et al. 2006

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The Wnt and Notch signalling pathways regulate hair follicle maintenance,but how they intersect is unknown. We show that Notch signalling is active in the hair follicle pre-cortex, a region of high Wnt activity, where commitment to hair lineages occurs. Deletion of jagged 1 (Jag1) results in inhibition of the hair growth cycle and conversion of hair follicles into cysts of cells undergoing interfollicular epidermal differentiation. Conversely, activation of Notch in adult epidermis triggers expansion of the base of the hair follicle, sebaceous gland enlargement and abnormal clumping of the follicles. In adult epidermis, the induction of new hair follicle formation by β-catenin is prevented by blocking Notch signalling pharmacologically or through Jag1 deletion. Conversely, activation of both pathways accelerates growth and differentiation of ectopic follicles.β-catenin stimulates Notch signalling by inducing Jag1transcription. We conclude that the Notch pathway acts downstream of the Wnt/β-catenin pathway to determine epidermal cell fate.

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Section: Research Articlementioning

confidence: 60%

Jagged 1 is a β-catenin target gene required for ectopic hair follicle formation in adult epidermis

et al. 2006

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“…Importantly, 38 of these genes are reportedly overrepresented in mouse DP cells (Fig. 2C) (Sleeman et al, 2000;Botchkarev and Kishimoto, 2003;Panteleyev et al, 2003;O'Shaughnessy et al, 2004;Rendl et al, 2005;Driskell et al, 2009). Most of them were established DP signature genes (Botchkarev and Kishimoto, 2003;Rendl et al, 2005;Ito et al, 2007;Osada et al, 2007;Driskell et al, 2009;Ohyama et al, 2010), including those encoding key molecules that enhance [e.g.…”

Section: Resultsmentioning

confidence: 94%

Restoration of the intrinsic properties of human dermal papilla in vitro

Ohyama

Kobayashi

Sasaki

et al. 2012

Journal of Cell Science

129

189

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SummaryThe dermal papilla (DP) plays pivotal roles in hair follicle morphogenesis and cycling. However, characterization and/or propagation of human DPs have been unsatisfactory because of the lack of efficient isolation methods and the loss of innate characteristics in vitro. We hypothesized that culture conditions sustaining the intrinsic molecular signature of the human DP could facilitate expansion of functional DP cells. To test this, we first characterized the global gene expression profile of microdissected, non-cultured human DPs. We performed a 'two-step' microarray analysis to exclude the influence of unwanted contaminants in isolated DPs and successfully identified 118 human DP signature genes, including 38 genes listed in the mouse DP signature. The bioinformatics analysis of the DP gene list revealed that WNT, BMP and FGF signaling pathways were upregulated in intact DPs and addition of 6-bromoindirubin-39-oxime, recombinant BMP2 and basic FGF to stimulate these respective signaling pathways resulted in maintained expression of in situ DP signature genes in primarily cultured human DP cells. More importantly, the exposure to these stimulants restored normally reduced DP biomarker expression in conventionally cultured DP cells. Cell growth was moderate in the newly developed culture medium. However, rapid DP cell expansion by conventional culture followed by the restoration by defined activators provided a sufficient number of DP cells that demonstrated characteristic DP activities in functional assays. The study reported here revealed previously unreported molecular mechanisms contributing to human DP properties and describes a useful technique for the investigation of human DP biology and hair follicle bioengineering.

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“…This mutation is located in the signal sequence and impairs protein transport to the plasma membrane. The finding that APCDD1 is a direct Wnt/b-catenin target (Takahashi et al 2002), and its similarity in expression with Wise (O'Shaughnessy et al 2004), encouraged Shimomura et al to analyze if APCDD1 may function as a Wnt inhibitor. They found that soluble APCDD1 binds to Wnt3a and to the extracellular domain of LRP5 in vitro, and it inhibits Wnt/b-catenin signaling in cultured cells, possibly by preventing Fz from binding to Wnt.…”

Section: Apcdd1mentioning

confidence: 99%