The World Saffron and Crocus collection: strategies for establishment, management, characterisation and utilisation

Fernández, José Antonio; Santana, Omar; Guardiola, J. L.; Molina, Rosa Victoria; Heslop-Harrison, J. S.; Borbély, György; Branca, F.; Argento, S.; Maloupa, Eleni; Talou, Thierry; Thiercelin, Jean Marie; Gasimov, Khalil; Vurdu, Hasan; Roldán, Marta; Santaella, Marcela; Sanchis, Enrique José; García-Luis, Amparo; Surányi, Gyula; Molnár, Attila; Sramkó, Gábor; Gulyás, G.; Balazs, Luckacs; Horvat, Orsolya; Rodríguez, María Fernanda; Sánchez‐Vioque, Raúl; Escolano, Miguel Ángel; Reina, José Vicente; Krigas, Nikos; Pastor, T.; Renau‐Morata, Begoña; Raynaud, Christine; Ibadli, Oruc; Polissiou, Moschos G.; Tsimidou, Maria Z.; Tsaftaris, Athanasios; Sharafeldin, M. A.; Medina, Joaquı́n; Constantinidis, Theophanis; Karamplianis, Theophanis; De-Los-Mozos-Pascual, M.

doi:10.1007/s10722-010-9601-5

Cited by 52 publications

(38 citation statements)

References 19 publications

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“…Corms of each species were collected in 2007 as part of a Crocus genebank collection program directed by the European Commission and called AGRI GEN RES 18 "CROCUSBANK" action (Fernández et al, 2011) that allowed the creation of a large collection of saffron crocus and its relatives. Two or three corms of the species involved in this study were further cultivated in the Botanical Garden of UD.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The genus Crocus includes a considerable number of wild-growing species, several of them being endangered, thus their conservation is important (Fernández et al, 2011). The species involved in our study were: (i) species taxonomically belonging to the "Verni" series: C. scepusiensis Borbás Ex Kulczyński endemic to Slovakia and Southern Poland, the Northern Carpathians, C. tommasinianus Herbert native to Hungary and the Balkans, C. vittatus Schlosser & Vukotinović native to Croatia and Hungary (C. heuffelianus Herbert, another member of this taxonomical series, has already been successfully cultured in vitro in our laboratory, see Demeter et al, 2010); (ii) the taxonomically distinct C. banaticus Gay, native to the Banat (Romania, Serbia), as well as Romanian and Ukrainian regions of the Carpathian Basin.…”

Section: Introductionmentioning

confidence: 99%

“…The genetic diversity of saffron is decreasing, partly because, being a triploid hybrid, it does not reproduce sexually (Fernández et al, 2011). Thus, tissue culture may offer a good method to preserve valued genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Tissue Cultures of Four Crocus Species from the Carpathian Basin

Freytag¹,

Pabar²,

Demeter³

et al. 2017

Acta Biologica Cracoviensia S. Botanica

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We aimed to produce tissue cultures and plant regeneration from endangered Crocus species: C. scepusiensis, C. tommasinianus, C. vittatus ("Verni" series of the genus) and C. banaticus. For initiation of cultures we used a plant growth regulator (PGR) combination used for in vitro culture of saffron and its relatives: 10 mg L -1 α-naphthaleneacetic acid (NAA) and 1 mg L -1 6-benzyladenine (BA). Shoot tips of young seedlings (C. scepusiensis) and corms (for the rest of species) were used as explants. C. scepusiensis explants developed into organogenic calli. On media with decreased NAA and with or without increased BA concentration, calli produced stigma-like structures and/or shoots and whole plants. In the other species, callus initiation medium induced callus formation with abundant somatic embryos. In C. tommasinianus, embryos developed shoots when auxin content of medium was decreased. In C. banaticus, a decrease of auxin with or without an increase in cytokinin content led to shoot or whole plant regeneration, as in C. scepusiensis. In the case of C. vittatus and C. banaticus, initiation and/or maintenance of cultures on indole-3-butyric acid (IBA) and increased sucrose concentration stimulated whole plant regeneration and in vitro cormlet development. C. scepusiensis and the rest of cultures (organogenic vs. embryogenic) differed at the biochemical level: C. scepusiensis cultures had higher (yet still low) enzymatic antioxidant (catalase, peroxidase) activities. With respect to catalase isoenzyme patterns, C. banaticus was different from the rest of cultures, demonstrating its distinct taxonomical position. Besides germplasm preservation use of the present cultures, they have a potential biotechnological value.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Tissue Cultures of Four Crocus Species from the Carpathian Basin

Freytag¹,

Pabar²,

Demeter³

et al. 2017

Acta Biologica Cracoviensia S. Botanica

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“…The genus Crocus (family Iridaceae ; subfmamily Crocoideae ) consists of many species (∼85–100) that occur in the wild and are distributed in the Central and Southern Europe (mainly in the Balkan Peninsula), North Africa and western Asia . Many crocuses are currently used as ornamental plants in gardens and parks for their colorful flowers whereas Crocus sativus L. is the only species cultivated for edible purpose (, http://www.saffronomics.org/). The latter one is a triploid and genetically sterile plant that is propagated vegetatively by corms.…”

Section: Saffron: the Dried Red Stigmas Of Crocus Sativus Linnaeusmentioning

confidence: 99%

Latest advances in the extraction and determination of saffron apocarotenoids

Kyriakoudi

2018

Electrophoresis

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Saffron, the dried red stigmas of Crocus sativus L. plant, is the most expensive spice in the world. It is highly valued not only for the color and flavor that exerts to various foods and drinks but also for its functional properties. Various classes of apocarotenoids such as crocetin sugar esters, picrocrocin and safranal are responsible for these characteristics. In the present review, the evolution of the methods proposed by the ISO standard for extraction and determination of saffron apocarotenoids since 1980 is presented in parallel to other approaches proposed by various scientists to overcome limitations of the standard. Moreover, the latest advances regarding applications of novel extraction techniques and powerful analytical tools that require limited or no sample preparation are critically discussed.

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“…saffron) and their progenitors (e.g. plants in the genus Crocus) may also be considered as target plants for the ex situ conservation (Fernández et al, 2011).…”

Section: Introductionmentioning

confidence: 99%