The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis

Dunphy, William G.; Brizuela, Leonardo; Beach, David; Newport, John W.

doi:10.1016/0092-8674(88)90205-x

Cited by 813 publications

(388 citation statements)

References 56 publications

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“…Moreover, cyclin B1, in complex with cdk1, functions as an M-phase kinase [37]. No changes of cyclin B1 level were observed in both cell lines except a distinct drop at 50µM ZOL in EGI-1 cells.…”

Section: Expression Of Putative Phase Markers and Cell Cycle Regulatorsmentioning

confidence: 87%

Zoledronic acid determines S-phase arrest but fails to induce apoptosis in cholangiocarcinoma cells

Romani

Desenzani

Morganti

et al. 2009

Biochemical Pharmacology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. In conclusion, our study indicates that zoledronic acid induces S-phase arrest and cell-narrowing, both reversed by GGOH and, by changing the delicate balance between pro-apoptotic and antiapoptotic proteins, allows survival of cholangiocarcinoma cells.

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Section: Expression Of Putative Phase Markers and Cell Cycle Regulatorsmentioning

confidence: 87%

Zoledronic acid determines S-phase arrest but fails to induce apoptosis in cholangiocarcinoma cells

Romani

Desenzani

Morganti

et al. 2009

Biochemical Pharmacology

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“…P34 cdc2 of almost any organism have been found able to associate with yeast p13SUC1 for their conservation [13]. Recent research revealed that p13 could also absorb directly or indirectly the subunits of cyclosome [14].…”

Section: Discussionmentioning

confidence: 99%

“…p13 conjugated affinity columns could absorb p34 cdc2 and some other Cdk proteins, alone or in a united form with its regulatory subunit cyclins [12], [13]. Phosphorylated forms of the cyclosome from clam oocytes, one around 1, 500 kDa complex with a regulated cyclin-ubiquitin ligase activity that targets cyclin B for degradation, was reported to bind to p13SUC1 [14] In this paper, using p13-agarose affinity methods, a protein of 28 kDa nominated as p28, was among the isolated proteins of lower molecular weights than those of cyclosome subunits.…”

Section: Introductionmentioning

confidence: 99%

A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation

et al. 2002

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ABSTRACTp28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13 suc1 -agarose affinity chromatography. Sequence homology analysis of the full-length cDNA of p28(Gene Bank accession number: AF 314091) indicated that it encodes a protein containing 224 amino-acids with about 55% identities and more than 70% positives to human, rat or mouse UCH-L1, and contains homological functional domains of UCH family. Anti-p28 monoclonal antibody, on injecting into the oocytes, could inhibit the progesterone-induced resumption of meiotic division in a dose-dependent manner. The recombinant protein p28 showed similar SDS/PAGE behaviors to the native one, and promoted ubiquitin ethyl ester hydrolysis, a classical catalytic reaction for ubiquitin carboxyl terminal hydrolases (UCHs). The results in this paper reveal that a novel protein, p28, exists in the toad oocytes, is a UCH L1 homolog, was engaged in the process of progesterone-induced oocyte maturation possibly through an involvement in protein turnover and degradation.

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“…The universal driver for the G 2 /M transition is the M-phase promoting factor MPF. MPF is a heterodimer composed of a regulatory cyclin subunit, cyclin B, and a catalytic subunit, Cdc2 kinase (Dunphy et al, 1988;Gautier et al, 1988;Nurse, 1990). Active Cdc2 drives entry into M-phase by phosphorylating substrates that lead to nuclear envelope breakdown (lamins A and B in vertebrates) and spindle formation (nucleolin; Belenguer et al, 1990;Heald and McKeon, 1990;Peter et al, 1990a,b).…”

Section: Cell Cycle Resumptionmentioning

confidence: 99%

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Horner

Wolfner

2008

Developmental Dynamics

183

177

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The transition from mature oocyte to developing embryo requires a coordinated series of events, collectively known as egg activation. Egg activation includes changes to egg coverings to prevent polyspermy, release of oocyte meiotic arrest, generation of haploid female and male pronuclei, changes in maternal mRNAs and protein populations, and cytoskeletal rearrangements. In many animals, egg activation is triggered by fertilization, which increases intracellular calcium within the oocyte and thereby regulates molecular events of egg activation. In other animals, fertilization-independent external signals, including mechanical stimulation of eggs and/or changes in ionic milieu, trigger activation. Recent studies have clarified the upstream portion of pathways leading to eggshell changes and cell cycle resumption and have identified activation-induced changes in maternal mRNA and protein profiles that can identify molecular players in the downstream events of egg activation. We review signals that trigger activation and how they link to subsequent molecular events of egg activation. Developmental Dynamics 237:527-544, 2008.

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The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis

Cited by 813 publications

References 56 publications

Zoledronic acid determines S-phase arrest but fails to induce apoptosis in cholangiocarcinoma cells

Zoledronic acid determines S-phase arrest but fails to induce apoptosis in cholangiocarcinoma cells

A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

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