The Xenopus Oocyte: A Tool for Membrane Biology

Limon, Agenor; Mattei, César

doi:10.3390/membranes13100831

Membranes

2023

DOI: 10.3390/membranes13100831

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The Xenopus Oocyte: A Tool for Membrane Biology

Agenor Limon,

César Mattei

Abstract: The Xenopus is a special study model in experimental research [...]

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2024

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Effect of Synthetic Vitreous Fiber Exposure on TMEM16A Channels in a Xenopus laevis Oocyte Model

Zangari,

Zabucchi,

Conti

et al. 2024

IJMS

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Many years ago, asbestos fibers were banned and replaced by synthetic vitreous fibers because of their carcinogenicity. However, the toxicity of the latter fibers is still under debate, especially when it concerns the early fiber interactions with biological cell membranes. Here, we aimed to investigate the effects of a synthetic vitreous fiber named FAV173 on the Xenopus laevis oocyte membrane, the cell model we have already used to characterize the effect of crocidolite asbestos fiber exposure. Using an electrophysiological approach, we found that, similarly to crocidolite asbestos, FAV173 was able to stimulate a chloride outward current evoked by step membrane depolarizations, that was blocked by the potent and specific TMEM16A channel antagonist Ani9. Exposure to FAV173 fibers also altered the oocyte cell membrane microvilli morphology similarly to crocidolite fibers, most likely as a consequence of the TMEM16A protein interaction with actin. However, FAV173 only partially mimicked the crocidolite fibers effects, even at higher fiber suspension concentrations. As expected, the crocidolite fibers’ effect was more similar to that induced by the co-treatment with (Fe3+ + H2O2), since the iron content of asbestos fibers is known to trigger reactive oxygen species (ROS) production. Taken together, our findings suggest that FAV173 may be less harmful that crocidolite but not ineffective in altering cell membrane properties.

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Effect of Synthetic Vitreous Fiber Exposure on TMEM16A Channels in a Xenopus laevis Oocyte Model

Zangari,

Zabucchi,

Conti

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

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The Xenopus Oocyte: A Tool for Membrane Biology

Abstract: The Xenopus is a special study model in experimental research [...]

Cited by 1 publication

References 17 publications

Effect of Synthetic Vitreous Fiber Exposure on TMEM16A Channels in a Xenopus laevis Oocyte Model

Effect of Synthetic Vitreous Fiber Exposure on TMEM16A Channels in a Xenopus laevis Oocyte Model

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