The zebrafish as a model to study the inflammasome

Angosto-Bazarra, Diego; Mulero, Víctoriano

doi:10.2478/infl-2014-0002

Cited by 9 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inflammasomes are intracellular multiprotein complexes that activate enzymes (caspases) through the receptors stimulation, such as the Pamps and Damps ("damage-associated molecular patterns"), and will promote inflammation. ROS formed in this system are part of biochemical reactions of signaling, and can also activate inflammation, via inflammasomes, which leads to the production of various proinflammatory immune cytokines such as interleukins (Paiva-Oliveira et al 2010, Tschopp and Schroder 2010, Angosto and Mulero 2014. "NOD like" receptors (NLRs) of fish have been used as biological inflammasomes model in order NLRs are Pamps and Damps receptors that induce the ROS production.…”

Section: Oxidative Stress and Immune System Of Fishmentioning

confidence: 99%

“…Currently, several studies have evaluated the effects of FR on the immune system and new areas of knowledge are being explored, such as immunosenescence, which studies the aging of cells of the immune system caused by FR, as well as inflammasomes, which are biochemical pathways initiated by the activation of receptors that provoke chronic inflammation (genesis of many diseases) (Paiva-Oliveira et al 2010, Tschopp and Schroder 2010, Angosto and Mulero 2014.…”

Section: Introductionmentioning

confidence: 99%

“…The concept of fish immune system(Biller-Takahashi and Urbinati 2014). JAQUELINE D. BILLER and LEONARDO S. TAKAHASHI to understand their evolution(Paiva-Oliveira et al 2010, Tschopp and Schroder 2010, Angosto and Mulero 2014.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity

Biller

Takahashi

2018

An. Acad. Bras. Ciênc.

213

View full text Add to dashboard Cite

Molecular oxygen is a necessary compound for all aerobic organisms, although oxygen is a potent oxidant, which can cause oxidative stress (OS). OS occurs when there is an imbalance between the production of oxidant and antioxidants components, are result of normal cell metabolism, and many of these compounds play a fundamental role in several metabolic pathways. The organism produces several reactive oxygen species (ROS), but they are balanced by an antioxidant defense system that maintains the levels of these oxidizing compounds at an acceptable level. Many of these components are essential in the organism defense and their byproducts are considered potent bactericides that actively act in the destruction of invading pathogens. Fish immune system is composed of innate and acquired mechanisms of defense. Phagocytosis is an innate process of defense, which interconnects these two systems, since the pathogens processing by professional phagocytes is a fundamental stage for antibodies production. During phagocytosis there is production of ROS and consequent production of free radicals (FR), these compounds lead to the formation of potent bactericides to combat microorganisms. However, it is known that OS limits the immune response, with an impairment in defense compounds in an attempt to decrease the ROS production. Studies of fish FR production are preliminary and should be executed to evaluate the effects of ROS on fish, including their beneficial action against pathogens and its deleterious action on the oxidation of cellular components.

show abstract

Section: Oxidative Stress and Immune System Of Fishmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity

Biller

Takahashi

2018

An. Acad. Bras. Ciênc.

213

View full text Add to dashboard Cite

show abstract

“…Importantly, while the study of fish models is likely to be useful in the study of the evolutionary biology of host-microbiome interactions, the availability of behavioural assays for anxiety in both adult and larval zebrafish offers potential biomedical applications. The development of models for stress-induced dysbiosis, novel developments regarding the zebrafish inflammasome (Angosto & Mulero, 2014) and the interactions between these mechanisms pave the way for innovative models for psychiatric disorders. In addition, fish are emerging models of social behaviour and associated pathological alterations.…”

Section: Con Cluding Remark Smentioning

confidence: 99%

Using fish models to investigate the links between microbiome and social behaviour: The next step for translational microbiome research?

et al. 2019

View full text Add to dashboard Cite

Recent research has revealed surprisingly important connections between animals’ microbiome and social behaviour. Social interactions can affect the composition and function of the microbiome; conversely, the microbiome affects social communication by influencing the hosts’ central nervous system and peripheral chemical communication. These discoveries set the stage for novel research focusing on the evolution and physiology of animal social behaviour in relation to microbial transmission strategies. Here, we discuss the emerging roles of teleost fish models and their potential for advancing research fields, linked to sociality and microbial regulation. We argue that fish models, such as the zebrafish (Danio rerio, Cyprinidae), sticklebacks (‎Gasterosteidae), guppies (Poeciliidae) and cleaner–client dyads (e.g., obligate cleaner fish from the Labridae and Gobiidae families and their visiting clientele), will provide valuable insights into the roles of microbiome in shaping social behaviour and vice versa, while also being of direct relevance to the food and ornamental fish trades. The diversity of fish behaviour warrants more interdisciplinary research, including microbiome studies, which should have a strong ecological (field‐derived) approach, together with laboratory‐based cognitive and neurobiological experimentation. The implications of such integrated approaches may be of translational relevance, opening new avenues for future investigation using fish models.

show abstract

“…Although their occurrence and existence in humans and other mammalian species are numerously investigated, those of inflammasomes in ancient vertebrates, such as teleost fish, remain poorly understood. Several previous studies have shown that there are two homologs of proinflammatory caspase, namely Danio rerio Caspase (DrCaspase)-A (Caspy) and DrCaspase-B (Caspy2) in zebrafish (20,21). The pro-IL-1b proteins in fish (as well as in amphibian and bird) lack a conserved caspase-1 recognition site, implying the existence of a unique mechanism for IL-1b maturation in lower vertebrates (22,23).…”

mentioning

confidence: 99%

Characterization of an NLRP1 Inflammasome from Zebrafish Reveals a Unique Sequential Activation Mechanism Underlying Inflammatory Caspases in Ancient Vertebrates

Gao

Shao

et al. 2018

The Journal of Immunology

View full text Add to dashboard Cite

NLRP1 inflammasome is one of the best-characterized inflammasomes in humans and other mammals. However, the existence of this inflammasome in nonmammalian species remains poorly understood. In this study, we report the molecular and functional identification of an NLRP1 homolog, NLRP1 (NLRP1) from a zebrafish () model. This NLRP1 possesses similar structural architecture to mammalian NLRP1s. It can trigger the formation of a classical inflammasome for the activation of zebrafish inflammatory caspases ( Caspase [Caspase]-A and Caspase-B) and maturation of IL-1β in a ASC (ASC)-dependent manner. In this process, NLRP1 promotes the aggregation ofASC into a filament with ASC core and ASC cluster. The assembly of NLRP1 inflammasome depends on the CARD-CARD homotypic interaction betweenNLRP1 and ASC core, and PYD-PYD interaction between Caspase-A/B andASC cluster. The FIIND domain in NLRP1 is necessary for inflammasome assembly. To understand the mechanism of how the twoCaspases are coordinated in NLRP1 inflammasome, we propose a two-step sequential activation model. In this model, the recruitment and activation ofCaspase-A/B in the inflammasome is shown in an alternate manner, with a preference for Caspase-A followed by a subsequent selection forCaspase-B. By using morpholino oligonucleotide-based knockdown assays, the NLRP1 inflammasome was verified to play important functional roles in antibacterial innate immunity in vivo. These observations demonstrate that the NLRP1 inflammasome originated as early as in teleost fish. This finding not only gives insights into the evolutionary history of inflammasomes but also provides a favorable animal model for the study of NLRP1 inflammasome-mediated immunology and diseases.

show abstract

The zebrafish as a model to study the inflammasome

Cited by 9 publications

References 23 publications

Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity

Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity

Using fish models to investigate the links between microbiome and social behaviour: The next step for translational microbiome research?

Characterization of an NLRP1 Inflammasome from Zebrafish Reveals a Unique Sequential Activation Mechanism Underlying Inflammatory Caspases in Ancient Vertebrates

Contact Info

Product

Resources

About