The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Klem, John R.; Gray, Raèden; Lovely, C. Ben

doi:10.3791/64679

Cited by 2 publications

(1 citation statement)

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“…As for studying the brain, zebrafish have another major advantage in being transparent as embryos and larvae and highly genetically tractable, allowing methods of transgenesis to be well established for labeling neuronal populations with fluorescent proteins [119][120][121]. Together, these features permit zebrafish to be tracked over time under a microscope using time-lapse live imaging and then examined for the effects of embryonic drug exposure on the in vivo development of neurons [122].…”

Section: Zebrafish Studies Of Embryonic Exposure To Drugs Of Abuse 41...mentioning

confidence: 99%

Utility of the Zebrafish Model for Studying Neuronal and Behavioral Disturbances Induced by Embryonic Exposure to Alcohol, Nicotine, and Cannabis

Collier,

Abdulai,

Leibowitz

2023

Cells

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It is estimated that 5% of pregnant women consume drugs of abuse during pregnancy. Clinical research suggests that intake of drugs during pregnancy, such as alcohol, nicotine and cannabis, disturbs the development of neuronal systems in the offspring, in association with behavioral disturbances early in life and an increased risk of developing drug use disorders. After briefly summarizing evidence in rodents, this review focuses on the zebrafish model and its inherent advantages for studying the effects of embryonic exposure to drugs of abuse on behavioral and neuronal development, with an emphasis on neuropeptides known to promote drug-related behaviors. In addition to stimulating the expression and density of peptide neurons, as in rodents, zebrafish studies demonstrate that embryonic drug exposure has marked effects on the migration, morphology, projections, anatomical location, and peptide co-expression of these neurons. We also describe studies using advanced methodologies that can be applied in vivo in zebrafish: first, to demonstrate a causal relationship between the drug-induced neuronal and behavioral disturbances and second, to discover underlying molecular mechanisms that mediate these effects. The zebrafish model has great potential for providing important information regarding the development of novel and efficacious therapies for ameliorating the effects of early drug exposure.

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Section: Zebrafish Studies Of Embryonic Exposure To Drugs Of Abuse 41...mentioning

confidence: 99%

Utility of the Zebrafish Model for Studying Neuronal and Behavioral Disturbances Induced by Embryonic Exposure to Alcohol, Nicotine, and Cannabis

Collier,

Abdulai,

Leibowitz

2023

Cells

View full text Add to dashboard Cite

show abstract

Clustered Regularly Interspaced Short Palindromic Repeats and Clustered Regularly Interspaced Short Palindromic Repeats–Associated Protein 9 System: Factors Affecting Precision Gene Editing Efficiency and Optimization Strategies

Li,

Tang,

Liang

et al. 2023

Human Gene Therapy

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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Cited by 2 publications

References 0 publications

Utility of the Zebrafish Model for Studying Neuronal and Behavioral Disturbances Induced by Embryonic Exposure to Alcohol, Nicotine, and Cannabis

Utility of the Zebrafish Model for Studying Neuronal and Behavioral Disturbances Induced by Embryonic Exposure to Alcohol, Nicotine, and Cannabis

Clustered Regularly Interspaced Short Palindromic Repeats and Clustered Regularly Interspaced Short Palindromic Repeats–Associated Protein 9 System: Factors Affecting Precision Gene Editing Efficiency and Optimization Strategies

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