Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study

Yang, Huohai; Aprecio, Raydolfo M.; Zhou, Xiaodong; Wang, Qi; Zhang, Wu; Ding, Yi; Li, Yiming

doi:10.1371/journal.pone.0100285

Cited by 63 publications

(61 citation statements)

References 37 publications

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“…iPS cells have also shown, in vitro, expression of periodontal tissue markers associated with bone, periodontal ligament and cementum under the influence of enamel matrix derivative (EMD) and growth/differentiation factor-5 (GDF-5) (42). Treatment of periodontitis with iPSC-MSC transfected with tumor necrosis factor gene 6 (TSG-6) have shown significant reduction of periodontal inflammation, with reduced levels of inflammatory infiltrates, proinflammatory cytokines and also inhibited the level of alveolar bone loss (43). …”

Section: Applications Ips Cells In Dentistry (Fig 1)mentioning

confidence: 99%

Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

Malhotra

2016

IJSC

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iPS cells are derived from somatic cells via transduction and expression of selective transcription factors. Both viral-integrating (like retroviral) and non-integrating (like, mRNA or protein-based) techniques are available for the production of iPS cells. In the field of dentistry, iPS cells have been derived from stem cells of apical papilla, dental pulp stem cells, and stem cells from exfoliated deciduous teeth, gingival and periodontal ligament fibroblasts, and buccal mucosa fibroblasts. iPS cells have the potential to differentiate into all derivatives of the 3 primary germ layers i.e. ectoderm, endoderm, and mesoderm. They are autogeneically accessible, and can produce patient-specific or disease-specific cell lines without the issue of ethical controversy. They have been successfully tested to produce mesenchymal stem cells-like cells, neural crest-like cells, ameloblasts-like cells, odontoblasts-like cells, and osteoprogenitor cells. These cells can aid in regeneration of periodontal ligament, alveolar bone, cementum, dentin-pulp complex, as well as possible Biotooth formation. However certain key issues like, epigenetic memory of iPS cells, viral-transduction, tumorgenesis and teratoma formation need to be overcome, before they can be successfully used in clinical practice. The article discusses the sources, pros and cons, and current applications of iPS cells in dentistry with an emphasis on encountered challenges and their solutions.

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Section: Applications Ips Cells In Dentistry (Fig 1)mentioning

confidence: 99%

Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

Malhotra

2016

IJSC

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“…Much research has been focused on investigating the potential of dental‐derived postnatal stem cells, periodontal ligament stem cells, dental pulp‐derived stem cells, stem cells from exfoliated deciduous teeth, dental follicle‐derived stem cells, dental apical papilla stem cells, and dental socket‐derived stem cells . In addition, studies have used induced pluripotent stem cells for periodontal regeneration . Literature suggests that the safest approach to cell therapy is autologous transplantation.…”

Section: Discussionmentioning

confidence: 99%

Cementum protein 1 transfection does not lead to ultrastructural changes in nucleolar organization of human gingival fibroblasts

Villegas-Mercado

Agredano-Moreno

Bermúdez

et al. 2018

J of Periodontal Research

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“…Recently, Yang et al . demonstrated that tumour necrosis factor alpha‐stimulated gene‐6 (TSG6) expressing iMSCs were capable of decreasing the inflammation in experimental periodontitis model and inhibiting alveolar bone resorption . Human MSCs have emerged as a promising therapeutic source for treating myocardial and limb ischaemia .…”

Section: Therapeutic Applications Of Imscsmentioning

confidence: 99%

hiPSC‐derived iMSCs: NextGen MSCs as an advanced therapeutically active cell resource for regenerative medicine

Sabapathy

Kumar

2016

J Cellular Molecular Medi

101

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Mesenchymal stem cells (MSCs) are being assessed for ameliorating the severity of graft‐versus‐host disease, autoimmune conditions, musculoskeletal injuries and cardiovascular diseases. While most of these clinical therapeutic applications require substantial cell quantities, the number of MSCs that can be obtained initially from a single donor remains limited. The utility of MSCs derived from human‐induced pluripotent stem cells (hiPSCs) has been shown in recent pre‐clinical studies. Since adult MSCs have limited capability regarding proliferation, the quantum of bioactive factor secretion and immunomodulation ability may be constrained. Hence, the alternate source of MSCs is being considered to replace the commonly used adult tissue‐derived MSCs. The MSCs have been obtained from various adult and foetal tissues. The hiPSC‐derived MSCs (iMSCs) are transpiring as an attractive source of MSCs because during reprogramming process, cells undergo rejuvination, exhibiting better cellular vitality such as survival, proliferation and differentiations potentials. The autologous iMSCs could be considered as an inexhaustible source of MSCs that could be used to meet the unmet clinical needs. Human‐induced PSC‐derived MSCs are reported to be superior when compared to the adult MSCs regarding cell proliferation, immunomodulation, cytokines profiles, microenvironment modulating exosomes and bioactive paracrine factors secretion. Strategies such as derivation and propagation of iMSCs in chemically defined culture conditions and use of footprint‐free safer reprogramming strategies have contributed towards the development of clinically relevant cell types. In this review, the role of iPSC‐derived mesenchymal stromal cells (iMSCs) as an alternate source of therapeutically active MSCs has been described. Additionally, we also describe the role of iMSCs in regenerative medical applications, the necessary strategies, and the regulatory policies that have to be enforced to render iMSC's effectiveness in translational medicine.

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Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study

Cited by 63 publications

References 37 publications

Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

Cementum protein 1 transfection does not lead to ultrastructural changes in nucleolar organization of human gingival fibroblasts

hiPSC‐derived iMSCs: NextGen MSCs as an advanced therapeutically active cell resource for regenerative medicine

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