Therapeutic Potential and Main Methods of Obtaining Selenium Nanoparticles

Varlamova, Elena G.; Turovsky, Egor A.; Блинова, Е. В.

doi:10.3390/ijms221910808

Cited by 63 publications

(52 citation statements)

References 204 publications

(230 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In control cell cultures of the mouse cerebral cortex, 8 ± 3% of necrotic cells can be recorded in vitro by Day 10 ( Figure 5 E,F), but after 40 min of OGD, necrosis is recorded in 83 ± 11% of cells. It is known that SeNPs have pleiotropic effects [ 23 ] and, on the one hand, activate apoptotic processes in cancer cells [ 17 , 24 ], but on the other hand, they are capable of exerting neuroprotective effects [ 25 ]. In addition, we have shown that the protective effects of the anti-inflammatory cytokine, interleukin-10, and antioxidants increase in proportion to the incubation time and are largely realized through the protection of astrocytes [ 20 , 22 ].…”

Section: Resultsmentioning

confidence: 99%

The Mechanisms Underlying the Protective Action of Selenium Nanoparticles against Ischemia/Reoxygenation Are Mediated by the Activation of the Ca2+ Signaling System of Astrocytes and Reactive Astrogliosis

Varlamova

Turovsky

Babenko

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

In recent years, much attention has been paid to the study of the therapeutic effect of the microelement selenium, its compounds, especially selenium nanoparticles, with a large number of works devoted to their anticancer effects. Studies proving the neuroprotective properties of selenium nanoparticles in various neurodegenerative diseases began to appear only in the last 5 years. Nevertheless, the mechanisms of the neuroprotective action of selenium nanoparticles under conditions of ischemia and reoxygenation remain unexplored, especially for intracellular Ca2+ signaling and neuroglial interactions. This work is devoted to the study of the cytoprotective mechanisms of selenium nanoparticles in the neuroglial networks of the cerebral cortex under conditions of ischemia/reoxygenation. It was shown for the first time that selenium nanoparticles dose-dependently induce the generation of Ca2+ signals selectively in astrocytes obtained from different parts of the brain. The generation of these Ca2+ signals by astrocytes occurs through the release of Ca2+ ions from the endoplasmic reticulum through the IP3 receptor upon activation of the phosphoinositide signaling pathway. An increase in the concentration of cytosolic Ca2+ in astrocytes leads to the opening of connexin Cx43 hemichannels and the release of ATP and lactate into the extracellular medium, which trigger paracrine activation of the astrocytic network through purinergic receptors. Incubation of cerebral cortex cells with selenium nanoparticles suppresses ischemia-induced increase in cytosolic Ca2+ and necrotic cell death. Activation of A2 reactive astrocytes exclusively after ischemia/reoxygenation, a decrease in the expression level of a number of proapoptotic and proinflammatory genes, an increase in lactate release by astrocytes, and suppression of the hyperexcitation of neuronal networks formed the basis of the cytoprotective effect of selenium nanoparticles in our studies.

show abstract

Section: Resultsmentioning

confidence: 99%

The Mechanisms Underlying the Protective Action of Selenium Nanoparticles against Ischemia/Reoxygenation Are Mediated by the Activation of the Ca2+ Signaling System of Astrocytes and Reactive Astrogliosis

Varlamova

Turovsky

Babenko

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Since Se has pronounced therapeutic potential for the treatment of viral infections and other conditions such as cancer, Se nanomedicine has received a lot of attention. Se nanoparticles are known to have low toxicity with marked and selective cytotoxic effects with small quantities [ 174 ]. Additionally, Se nanoparticles have high effectiveness in the inhibition of oxidative damage [ 175 , 176 , 177 ].…”

Section: Viral Infections and Seleniummentioning

confidence: 99%

“…Recently published data show that Se nanoparticles activate programmed cell death in target cancer tissue through calcium (Ca) 2+ signaling pathways [ 178 ]. Immune cells also require calcium flux to generate oxidative stress [ 174 ]. Through chemical methods, Se nanoparticles may be produced with Se sources that include sodium selenite, selenious acid, and sodium selenosulfate [ 174 ].…”

Section: Viral Infections and Seleniummentioning

confidence: 99%

“…Immune cells also require calcium flux to generate oxidative stress [ 174 ]. Through chemical methods, Se nanoparticles may be produced with Se sources that include sodium selenite, selenious acid, and sodium selenosulfate [ 174 ]. Due to the developing relationship between Se and COVID-19, Se nanomedicine is being suggested as a tool in the fight against SARS-CoV-2 [ 179 ].…”

Section: Viral Infections and Seleniummentioning

confidence: 99%

See 1 more Smart Citation

Role of Selenium in Viral Infections with a Major Focus on SARS-CoV-2

Martínez

Huang

Acuña

et al. 2021

IJMS

View full text Add to dashboard Cite

Viral infections have afflicted human health and despite great advancements in scientific knowledge and technologies, continue to affect our society today. The current coronavirus (COVID-19) pandemic has put a spotlight on the need to review the evidence on the impact of nutritional strategies to maintain a healthy immune system, particularly in instances where there are limited therapeutic treatments. Selenium, an essential trace element in humans, has a long history of lowering the occurrence and severity of viral infections. Much of the benefits derived from selenium are due to its incorporation into selenocysteine, an important component of proteins known as selenoproteins. Viral infections are associated with an increase in reactive oxygen species and may result in oxidative stress. Studies suggest that selenium deficiency alters immune response and viral infection by increasing oxidative stress and the rate of mutations in the viral genome, leading to an increase in pathogenicity and damage to the host. This review examines viral infections, including the novel SARS-CoV-2, in the context of selenium, in order to inform potential nutritional strategies to maintain a healthy immune system.

show abstract

“…SeNPs are able to cross the blood–brain barrier, accumulate in the brain and prevent the development of apoptosis in cells 18 – 21 . The uniqueness of nanoparticles is mediated by their properties—small size, high surface area, surface charge, surface chemistry, solubility and multi-functionality 22 , 23 . Nanoparticles are ideal for biomedical use because they are small enough (10–100 nm) not to be absorbed by the reticuloendothelial system, but large enough not to be filtered out in the kidneys 24 – 26 .…”

Section: Introductionmentioning

confidence: 99%

Features of the cytoprotective effect of selenium nanoparticles on primary cortical neurons and astrocytes during oxygen–glucose deprivation and reoxygenation

Turovsky

Mal’tseva

Sarimov

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The study is aimed at elucidating the effect of selenium nanoparticles (SeNPs) on the death of cells in the primary culture of mouse cerebral cortex during oxygen and glucose deprivation (OGD). A primary cell culture of the cerebral cortex containing neurons and astrocytes was subjected to OGD and reoxygenation to simulate cerebral ischemia-like conditions in vitro. To evaluate the neuroprotective effect of SeNPs, cortical astrocytes and neurons were incubated for 24 h with SeNPs, and then subjected to 2-h OGD, followed by 24-h reoxygenation. Vitality tests, fluorescence microscopy, and real-time PCR have shown that incubation of primary cultured neurons and astrocytes with SeNPs at concentrations of 2.5–10 µg/ml under physiological conditions has its own characteristics depending on the type of cells (astrocytes or neurons) and leads to a dose-dependent increase in apoptosis. At low concentration SeNPs (0.5 µg/ml), on the contrary, almost completely suppressed the processes of basic necrosis and apoptosis. Both high (5 µg/ml) and low (0.5 µg/ml) concentrations of SeNPs, added for 24 h to the cells of cerebral cortex, led to an increase in the expression level of genes Bcl-2, Bcl-xL, Socs3, while the expression of Bax was suppressed. Incubation of the cells with 0.5 µg/ml SeNPs led to a decrease in the expression of SelK and SelT. On the contrary, 5 µg/ml SeNPs caused an increase in the expression of SelK, SelN, SelT, SelP. In the ischemic model, after OGD/R, there was a significant death of brain cells by the type of necrosis and apoptosis. OGD/R also led to an increase in mRNA expression of the Bax, SelK, SelN, and SelT genes and suppression of the Bcl-2, Bcl-xL, Socs3, SelP genes. Pre-incubation of cell cultures with 0.5 and 2.5 µg/ml SeNPs led to almost complete inhibition of OGD/R-induced necrosis and greatly reduced apoptosis. Simultaneously with these processes we observed suppression of caspase-3 activation. We hypothesize that the mechanisms of the protective action of SeNPs involve the activation of signaling cascades recruiting nuclear factors Nrf2 and SOCS3/STAT3, as well as the activation of adaptive pathways of ESR signaling of stress arising during OGD and involving selenoproteins SelK and SelT, proteins of the Bcl-2 family ultimately leading to inactivation of caspase-3 and inhibition of apoptosis. Thus, our results demonstrate that SeNPs can act as neuroprotective agents in the treatment of ischemic brain injuries.

show abstract

Therapeutic Potential and Main Methods of Obtaining Selenium Nanoparticles

Cited by 63 publications

References 204 publications

The Mechanisms Underlying the Protective Action of Selenium Nanoparticles against Ischemia/Reoxygenation Are Mediated by the Activation of the Ca2+ Signaling System of Astrocytes and Reactive Astrogliosis

The Mechanisms Underlying the Protective Action of Selenium Nanoparticles against Ischemia/Reoxygenation Are Mediated by the Activation of the Ca2+ Signaling System of Astrocytes and Reactive Astrogliosis

Role of Selenium in Viral Infections with a Major Focus on SARS-CoV-2

Features of the cytoprotective effect of selenium nanoparticles on primary cortical neurons and astrocytes during oxygen–glucose deprivation and reoxygenation

Contact Info

Product

Resources

About