Therapeutic Potential of Bone Marrow Derived Mesenchymal Stem Cells in Modulating Astroglyosis of Surgical Induced Experimental Spinal Cord Injury

Abstract

Background: Spinal cord injury (SCI) unsuccessful regeneration was due to glial scar development. It was a major obstacle to axonal restoration. Safe therapeutic intervention by the use of bone marrow derived stem cells (BMMSCs) transplantation applied in the present study could reduce spinal disability. Material and methods: Forty male albino rats were divided into four groups: GI: negative control (n = 10 rats); GII: positive control after SCI (n = 10 rats); GIII: SCI + BM − MSCs intravenous injected and GIV: SCI + BM − MSCs intra lesion injected (n = 10 rats in each group). The samples were taken from spinal cord tissues around the region of injury and were subjected to histological, immunohistochemical assessment. RNA extraction and real time PCR for detection of nerve regeneration and astrocyte response to the injury were also performed. Results: Clinical improvement occurred by the enhancement in the Basso, Beattie and Bresnahan (BBB) score after SCI. Histological examinations showed positive regenerative responses in GIV compared to GIII. Conclusion: BM-MSCs transplantation has a promising role in enhancing the microenvironment for nerve regeneration through stumbling the glial scaring formation and inflammatory response after chronic spinal cord injury especially by using intra-lesion route injection.

The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity

“…Several experimental studies have shown the beneficial effect of MSCs in testicular injuries induced by different agents [ 7 , 12 , 31 – 33 ]. This effect is mostly exerted via direct replacement of the damaged tissues and cell differentiation, or indirectly induces cell regeneration through paracrine signaling, which involves secretion of various mediators and growth factors that modulate the cellular interactions [ 16 , 20 , 34 , 35 ].…”

“…The BM cells were isolated as described before by Elawady et al, 2016 [ 20 ]. In brief, BM cells were flushed from tibia and fibula of rat bones with phosphate-buffered saline (PBS).…”

“…The MSCs were identified by their morphology, adherence, surface markers, and their capacity to differentiate into osteocytes and adipocytes as detected by an inverted microscope (Leica, Wetzlar, Germany). Cell surface antigen expression of BM-MSCs was analyzed by flow cytometry in which cells were incubated with fluorescein isothiocyanate-conjugated (anti-CD45) or phycoerythrin- cyanine-5-conjugated (anti-CD90) or rat immunoglobulin G (IgG) isotype control antibodies for 30 min at 4 °C in PBS [ 20 ].…”

Adipose-derived mesenchymal stem cells ameliorate the damages induced by arsenic trioxide in the testis of rats

“…In the present result, AD-MSCs improved the harmful effect of As III L & H in testicular tissues. However, a few investigations have indicated the gainful effect of MSCs in testicular injuries incited by various agents in different organs and animals (Cakici et al, 2015;Elawady et al, 2016). (Lue et al, 2007) suggested, the blood-testis barrier may help the MSCs to preserve from immunologic responses.…”