Therapeutically Active Marine Sponge Isolated from Rameshwaram Thoppu Kaadu Theevu against MDR Pathogen Lactobacillus acidophilus Isolated from Childhood Caries

Abstract

Dental illness is a foremost health problem upsetting childhood and this action, severely causes sickness which is caused by emerging pathogen Lactobacillus acidophilus. So plaque samples were collected from diverse dental clinics around Tirupur Dt. In this study, 20 MDR isolates were isolated and identified by biochemical analysis. Additionally, the decay pathogen Lactobacillus acidophilus was assayed against Levofloxacin, Moxifloxacin, Clindamycin, Vancomycin, Ampicillin, Chloramphenicol, Tetracycline and Novobiocin. Among these strains 0%, 0%, 26%, 34%, 22%, 2%, 16%, 16% and 6% were found to exhibit resistance to four groups of antibiotics. Currently, the extension of resistance to contemporary antimicrobials has demand to search for innovative anti-therapeutic agent. Moreover, several studies mostly deal with marine sponge to explore the therapeutic drug and this potential role in the manufacture of metabolites is becoming a driving area for exploring a rich source of novel compounds that are of prospective interest to mankind for diagnosis of tooth decay. Different concentrations of sponge 50μg, 100μg, and 150μg were prepared with DMSO, and well diffusion assay was used. The result indicated that the maximum and minimum inhibition of 22 mm and 16 mm was observed against the isolate LAVG07 and LAVG08. This research proved that the marine sponge extract shows the prominent antimicrobial activity against decay causing organism and it may be broadcast to be a successful approach to come to blow promising multi challenging clinical drugs in the future.