Thermal stability of the solid DNA as a novel optical material

Nizioł, Jacek; Makyła-Juzak, Katarzyna; Marzec, Mateusz; Ekiert, Robert; Marzec, M.; Gondek, E.

doi:10.1016/j.optmat.2017.02.035

Cited by 14 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used salmon testis DNA powder purchased from Ogata Research Laboratories Ltd. in Japan, which has been widely used in DNA-TSF fabrication [7,10,24,25]. The DNA powder was dissolved in deionized water to make homogeneous DNA aqueous solutions with a concentration of 0.4 wt.…”

Section: Methodsmentioning

confidence: 99%

Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films

Jeong¹,

Paulson²,

Hong³

et al. 2018

Photon. Res.

View full text Add to dashboard Cite

The denaturation of double-stranded deoxyribonucleic acid (ds-DNA) has been well known to break nucleobase bonds, resulting in single-stranded deoxyribonucleic acid (ss-DNA) in solutions, which can recombine to form ds-DNA in a reversible manner. We developed an efficient process to irreversibly maintain various DNA denaturation levels in thin solid films in order to investigate the impacts of the denaturation on the optical properties of DNA films. By adding NaOH in an aqueous solution of salmon testis DNA, we flexibly controlled the level of denaturation in the solution, which was then spin-coated on Si and silica substrates to irreversibly bind ss-DNAs in a thin solid film. The denaturation of DNA in thin solid films was experimentally confirmed by ultravioletvisible and Fourier transform infrared spectroscopic investigations, whose level could be controlled by the NaOH content in the aqueous solution precursor. By this irreversible denaturation process, we developed a new method to flexibly vary the refractive index of DNA thin solid films in a wide range of Δn > 0.02 in the visible to nearinfrared range. Thermo-optic coefficients dn/dT of the films were also experimentally measured in the temperature range from 40°C to 90°C to confirm the significant impacts of denaturation. Detailed thin film processes and optical characterizations are discussed.

show abstract

Section: Methodsmentioning

confidence: 99%

Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films

Jeong¹,

Paulson²,

Hong³

et al. 2018

Photon. Res.

View full text Add to dashboard Cite

show abstract

“…4(a) . Peaks at λ = 207, 257, and 275 ± 5 nm correspond to characteristic oscillators of DNA 22 , 36 , and their spectral positions were almost independent of riboflavin doping, as seen in the left inset of Fig. 4(a) .…”

Section: Resultsmentioning

confidence: 86%

“…%, while the riboflavin concentration varied from 0 to 130 μM, as riboflavin concentrations were limited by precipitation and crystallization above 150 μM. As spin coating and DNA thin films are known to be very sensitive to viscosity, hydration, and process history 19 , 22 , 41 , special care was taken that precursor solution concentrations be accurate to one part in 10 4 and that samples be subject to identical thermal, mixing, and humidity conditions.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Optical dispersion control in surfactant-free DNA thin films by vitamin B2 doping

Paulson

Shin

Jeong

et al. 2018

Sci Rep

View full text Add to dashboard Cite

A new route to systematically control the optical dispersion properties of surfactant-free deoxyribonucleic acid (DNA) thin solid films was developed by doping them with vitamin B2, also known as riboflavin. Surfactant-free DNA solid films of high optical quality were successfully deposited on various types of substrates by spin coating of aqueous solutions without additional chemical processes, with thicknesses ranging from 18 to 100 nm. Optical properties of the DNA films were investigated by measuring UV-visible-NIR transmission, and their refractive indices were measured using variable-angle spectroscopic ellipsometry. By doping DNA solid films with riboflavin, the refractive index was consistently increased with an index difference Δn ≥ 0.015 in the spectral range from 500 to 900 nm, which is sufficiently large to make an all-DNA optical waveguide. Detailed correlation between the optical dispersion and riboflavin concentration was experimentally investigated and thermo-optic coefficients of the DNA-riboflavin thin solid films were also experimentally measured in the temperature range from 20 to 85 °C, opening the potential to new bio-thermal sensing applications.

show abstract

“…It shows the viscosity with shear rate for each concentration, and the predicted viscosity at each DNA concentration is replotted, as shown in Figure 4 f. The predicted DNA viscosity at each time point when the DNA solution and the coagulated FITC are in contact is shown in Figure 4 g. During the manufacturing process of the DNA-based MNs, the applied temperature (50 °C) was selected to fabricate the MNs. At this temperature, the DNA is stable [ 33 ]. The viscosity increased rapidly.…”

Section: Resultsmentioning

confidence: 99%

Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles

et al. 2020

View full text Add to dashboard Cite

Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with efficient amounts of FITC as the drug model. Importantly, the vacuum process, which could influence the capturing of FITC diffusion from the tip, was evaluated during the manufacturing process. In addition, the simulations were consistent with the experimental results and showed apparent diffusion. Moreover, dextrans of different molecular weights labeled with FITC were chosen to fabricate the tip of microneedles for demonstrating their applicability. Finally, a micro-jetting system with a micro-nozzle (diameter: 80 μm) was developed to achieve the accurate and rapid loading of small amounts of FITC using the anti-diffusion and micro-jetting methods. Our method not only uses a simple and fast manufacturing process, but also fabricates the tips of microneedles more efficiently with FITC compared with the existing methods. We believe that the proposed method is essential for the clinical applications of the microneedle drug delivery platform.

show abstract

Thermal stability of the solid DNA as a novel optical material

Cited by 14 publications

References 30 publications

Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films

Irreversible denaturation of DNA: a method to precisely control the optical and thermo-optic properties of DNA thin solid films

Optical dispersion control in surfactant-free DNA thin films by vitamin B2 doping

Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles

Contact Info

Product

Resources

About