Thermally induced changes in the profiles of phytocannabinoids and other bioactive compounds in Cannabis sativa L. inflorescences

Benes, Frantisek; Binova, Zuzana; Zlechovcova, Marie; Maly, Matej; Stranska, Milena; Hajslova, Jana

doi:10.1016/j.foodres.2024.114487

Food Research International

2024

DOI: 10.1016/j.foodres.2024.114487

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Thermally induced changes in the profiles of phytocannabinoids and other bioactive compounds in Cannabis sativa L. inflorescences

Frantisek Benes,

Zuzana Binova,

Marie Zlechovcova

et al.

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2024

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Cited by 2 publications

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Critical Assessment of Clean‐up Procedures Used for Gas Chromatography‐Mass Spectrometry Analysis of Pesticide Residues in Cannabis Inflorescence

Mraz,

Drabova,

Stupak

et al. 2024

J of Separation Science

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Accurate analysis of pesticide residues in such a complex matrix as cannabis is a challenging task. The aim of this study was to find an optimal way of removing abundant matrix co‐extracts from Quick, Easy, Cheap, Effective, Rugged, and Safe extract prior to its analysis by gas chromatography‐tandem mass spectrometry (GC‐MS/MS). Out of the seven procedures tested, clean‐up with Supel QuE Verde sorbent provided the most satisfactory performance characteristics for 277 targeted pesticides, which is the highest number of these analytes ever investigated within a GC‐MS‐based study focused on cannabis. The criteria set in the SANTE/11312/2021 V2 document were considered to assess the results obtained within the method validation. Recoveries in the range of 70%–120% with repeatability ≤20% were obtained for 219 and 114 pesticides at spiking levels of 0.1 and 0.01 mg/kg, respectively. For a deeper insight into the function of tested sorbents and to understand the effects of interfering matrix co‐extracts, the two‐dimensional GC coupled with the time‐of‐flight MS method was employed for the analysis of all purified extracts. Co‐eluting major phytocannabinoids and free fatty acids were shown to be responsible for high “chemical noise”, increasing limits of quantification of pesticides at respective retention times. Since cannabis chemotypes fairly differ in phytocannabinoid content, the matrix effects, thus performance characteristics, may vary. Under such conditions, validation is recommended for different cannabis chemotypes.

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Critical Assessment of Clean‐up Procedures Used for Gas Chromatography‐Mass Spectrometry Analysis of Pesticide Residues in Cannabis Inflorescence

Mraz,

Drabova,

Stupak

et al. 2024

J of Separation Science

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Tea Prepared from Dried Cannabis: What Do We Drink?

Maly,

Benes,

Binova

et al. 2024

J. Agric. Food Chem.

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Besides many other uses, dried Cannabis may be used for “tea” preparation. This study focused on a comprehensive characterization of an aqueous infusion prepared according to a common practice from three fairly different Cannabis cultivars. The transfer of 42 phytocannabinoids and 12 major bioactive compounds (flavonoids) into the infusion was investigated using UHPLC-HRMS/MS. Phytocannabinoid acids were transferred generally in a higher extent compared to their counterparts; in the case of Δ9-THC, it was only in the range of 0.4–1.9% of content in the Cannabis used. A dramatic increase of phytocannabinoids, mainly of the neutral species, occurred when cream was added during steeping, and the transfer of Δ9-THC into “tea” achieved a range of 53–64%. Under such conditions, drinking a 250 mL cup of such tea by a 70 kg person might lead to multiple exceedance of the Acute Reference Dose (ARfD), 1 μg/kg b.w., even in the case when using hemp with a Δ9-THC content below 1% in dry weight for preparation.

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Thermally induced changes in the profiles of phytocannabinoids and other bioactive compounds in Cannabis sativa L. inflorescences

Cited by 2 publications

References 63 publications

Critical Assessment of Clean‐up Procedures Used for Gas Chromatography‐Mass Spectrometry Analysis of Pesticide Residues in Cannabis Inflorescence

Critical Assessment of Clean‐up Procedures Used for Gas Chromatography‐Mass Spectrometry Analysis of Pesticide Residues in Cannabis Inflorescence

Tea Prepared from Dried Cannabis: What Do We Drink?

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