Thermo-Sensitive Transient Receptor Potential Vanilloid (TRPV) Channels Regulate IL-6 Expression in Mouse Adipocytes

Abstract

IntroductionObesity exacts a continuing toll on public health and is a wellknown risk factor for diabetes, hypertension, hyperlipidemia, and cardiovascular diseases [1][2][3][4]. It is evident that not only hypertrophy of adipocytes and increase of fat deposit but also proliferation of preadipocytes and differentiation from pre-adipocytes to adipocytes have important roles for forming obesity. The most intensive and clinical interventions to prevent obesity have been primarily directed to decrease excessive amounts of fat tissue by a change in the balance between intake and expenditure of energy using daily exercise and diet control. On the other hand, the inverse correlation between body mass index (BMI) and the average temperature of January exists, and BMI is low when the temperature is high [5]. In addition, when rats were fed under low or high temperature environment, the amount of subcutaneous fat in rats fed under high temperature was significantly low, compared with that under the low temperature [5]. Thus, hyperthermia may affect adipose cell functions, then adipogenesis, and provides a therapeutic or preventive modality against obesity-related diseases. However, there are few reports concerning the direct effects of hyperthermia stimulation on adipocytes and its underlying mechanism. AbstractBackground: Hyperthermia may affect adipose cell functions and then adipogenesis. Thermo-sensitive transient receptor potential (TRPs) proteins such as TRPV1-4 play vital roles in cellular functions. We investigated the expression of thermo-sensitive TRPVs and their roles on IL-6 production in mouse 3T3-L1 preadipocytes and differentiated adipocytes.

TRPV4 modulation affects mitochondrial parameters in adipocytes and its inhibition upregulates lipid accumulation

“…Expression of different TRPV members, such as TRPV1, TRPV2, TRPV3, TRPV4 & TRPV6 in mouse adipocytes (3T3L-1) was reported [36]. Notably, relative expressions of TRPVs change in pre-adipocytes and mature adipocytes [36, 37]. TRPV4 expression alters during the process of adipogenesis.…”