Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge

Lim, Jae Kyu; Kim, Yun Jae; Yang, Jhung-Ahn; Namirimu, Teddy; Yang, Sung‐Hyun; Park, Mi-Jeong; Kwon, Yong Hyun; Lee, Hyun Sook; Kang, Sung Gyun; Lee, Jung-Hyun; Kwon, Kae Kyoung

doi:10.1007/s12275-020-9424-9

Cited by 15 publications

(8 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The heterotroph Hyperthermus hephaesta Ro04 isolated from the same area is also able to reduce Fe(III) (Lin et al 2014(Lin et al , 2016. Thermococcus indicus strain IOH1, T. celericrescens DSM 17994, and T. siculi DSM 12349 have also been reported to reduce soluble Fe(III)-citrate present in their growth medium, which improves their growth (Lim et al 2020).…”

Section: Archaea-euryarchaeotamentioning

confidence: 99%

Microorganisms from deep-sea hydrothermal vents

Zeng

Alain

Shao

2021

Mar Life Sci Technol

View full text Add to dashboard Cite

With a rich variety of chemical energy sources and steep physical and chemical gradients, hydrothermal vent systems offer a range of habitats to support microbial life. Cultivation-dependent and independent studies have led to an emerging view that diverse microorganisms in deep-sea hydrothermal vents live their chemolithoautotrophic, heterotrophic, or mixotrophic life with versatile metabolic strategies. Biogeochemical processes are mediated by microorganisms, and notably, processes involving or coupling the carbon, sulfur, hydrogen, nitrogen, and metal cycles in these unique ecosystems. Here, we review the taxonomic and physiological diversity of microbial prokaryotic life from cosmopolitan to endemic taxa and emphasize their significant roles in the biogeochemical processes in deep-sea hydrothermal vents. According to the physiology of the targeted taxa and their needs inferred from meta-omics data, the media for selective cultivation can be designed with a wide range of physicochemical conditions such as temperature, pH, hydrostatic pressure, electron donors and acceptors, carbon sources, nitrogen sources, and growth factors. The application of novel cultivation techniques with real-time monitoring of microbial diversity and metabolic substrates and products are also recommended.

show abstract

Section: Archaea-euryarchaeotamentioning

confidence: 99%

Microorganisms from deep-sea hydrothermal vents

Zeng

Alain

Shao

2021

Mar Life Sci Technol

View full text Add to dashboard Cite

show abstract

“…All dDDH values were below the 70 % dDDH species Table 1. Differential characteristics of strain SY113 T and related strains Strain: 1, SY113 T (this study); 2, Thermococcus celericrescens TS2 T [31]; 3, Thermococcus indicus IOH1 [22]; 4, Thermococcus siculi RG20 T [19]; 5, Thermococcus barossii [32]; 6, Thermococcus celer Vu13 T [33]; 7, Thermococcus coalescens TS1 T [34]; +, Positive; -, negative; w, weakly positive. boundary (Table S1).…”

Section: Genome Featuresmentioning

confidence: 87%

“…Interestingly, most of validly published deep-sea Thermococcus species have been obtained from the Northern Hemisphere, such as the East Pacific Rise [18], the Okinawa Trough [19], the Mid-Atlantic Ridge [20] and Guaymas Basin [17]. Only a few Thermococcus species have been obtained from deep-sea vents in the Southern Hemisphere, such as Thermococcus onnurineus NA1, isolated from the PACMANUS hydrothermal field [21], and Thermococcus indicus IOH1 isolated from the Central Indian Ocean ridge [22]. We describe here a novel species of the genus Thermococcus isolated from a deep-sea hydrothermal vent of the ultraslow spreading Southwest Indian Ridge (SWIR) in the Southern Hemisphere.…”

Section: Full-textmentioning

confidence: 99%

Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent chimney in the Southwest Indian Ridge

Tang

Zhang

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A hyperthermophilic, strictly anaerobic archaeon, designated strain SY113T, was isolated from a deep-sea hydrothermal vent chimney on the Southwest Indian Ridge at a water depth of 2770 m. Enrichment and isolation of strain SY113T were performed at 85 °C at 0.1 MPa. Cells of strain SY113T were irregular motile cocci with peritrichous flagella and generally 0.8–2.4 µm in diameter. Growth was observed at temperatures between 50 and 90 °C (optimum at 85 °C) and under hydrostatic pressures of 0.1–60 MPa (optimum, 27 MPa). Cells of SY113T grew at pH 4.0–9.0 (optimum, pH 5.5) and a NaCl concentration of 0.5–5.5 % (w/v; optimum concentration, 3.0 % NaCl). Strain SY113T was an anaerobic chemoorganoheterotroph and grew on complex proteinaceous substrates such as yeast extract and tryptone, as well as on maltose and starch. Elemental sulphur stimulated growth, but not obligatory for its growth. The G+C content of the genomic DNA was 55.0 mol%. Phylogenetic analysis of the 16S rRNA sequence of strain SY113T showed that the novel isolate belonged to the genus Thermococcus . On the basis of physiological characteristics, average nucleotide identity values and in silico DNA–DNA hybridization results, we propose a novel species, named Thermococcus aciditolerans sp. nov. The type strain is SY113T (=MCCC 1K04190T=JCM 39083T).

show abstract

“…Growth of strain was observed at NaCl concentrations between 2.0 and 6.0 %, with an optimum around 3.5–4.5 % (). Utilization of various carbon sources for growth was tested in MM1 medium as described previously [11]. The following substrates were tested as sole carbon sources at a final concentration of 5 g l −1 : d -(+)-glucose, maltodextrin, starch and pyruvate.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Thermococcus argininiproducens sp. nov., an arginine biosynthesis archaeal species isolated from the Central Indian Ocean ridge

Park

Lim

Kim

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

A strictly anaerobic hyperthermophilic archaeon, designated strain IOH2T, was isolated from a deep-sea hydrothermal vent (Onnuri vent field) area on the Central Indian Ocean Ridge. Strain IOH2T showed high 16S rRNA gene sequence similarity to Thermococcus sibiricus MM 739T (99.42 %), Thermococcus alcaliphilus DSM 10322T (99.28 %), Thermococcus aegaeus P5T (99.21 %), Thermococcus litoralis DSM 5473T (99.13 %), ‘Thermococcus bergensis’ T7324T (99.13 %), Thermococcus aggregans TYT (98.92 %) and Thermococcus prieurii Bio-pl-0405IT2T (98.01 %), with all other strains showing lower than 98 % similarity. The average nucleotide identity and in silico DNA–DNA hybridization values were highest between strain IOH2T and T. sibiricus MM 739T (79.33 and 15.00 %, respectively); these values are much lower than the species delineation cut-offs. Cells of strain IOH2T were coccoid, 1.0–1.2 µm in diameter and had no flagella. Growth ranges were 60–85 °C (optimum at 80 °C), pH 4.5–8.5 (optimum at pH 6.3) and 2.0–6.0 % (optimum at 4.0 %) NaCl. Growth of strain IOH2T was enhanced by starch, glucose, maltodextrin and pyruvate as a carbon source, and elemental sulphur as an electron acceptor. Through genome analysis of strain IOH2T, arginine biosynthesis related genes were predicted, and growth of strain IOH2T without arginine was confirmed. The genome of strain IOH2T was assembled as a circular chromosome of 1 946 249 bp and predicted 2096 genes. The DNA G+C content was 39.44 mol%. Based on the results of physiological and phylogenetic analyses, Thermococcus argininiproducens sp. nov. is proposed with type strain IOH2T (=MCCC 4K00089T=KCTC 25190T).

show abstract

Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge

Cited by 15 publications

References 50 publications

Microorganisms from deep-sea hydrothermal vents

Microorganisms from deep-sea hydrothermal vents

Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent chimney in the Southwest Indian Ridge

Thermococcus argininiproducens sp. nov., an arginine biosynthesis archaeal species isolated from the Central Indian Ocean ridge

Contact Info

Product

Resources

About