Thermococcus
            nautili sp. nov., a hyperthermophilic archaeon isolated from a hydrothermal deep-sea vent

Gorlas, Aurore; Croce, Olivier; Oberto, Jacques; Gauliard, Emilie; Forterre, Patrick; Marguet, Evelyne

doi:10.1099/ijs.0.060376-0

Cited by 27 publications

(17 citation statements)

References 75 publications

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“…AM4 and, when revising this manuscript, BD01_0811 from Thermococcus nautili . Both species are phylogenetically close to T. gammatolerans . Remarkably, they were all isolated from hydrothermal vents and are hyperthermophilic and anaerobic species.…”

Section: Resultsmentioning

confidence: 91%

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Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans

Yang

Fernandez²,

Lagorce

et al. 2015

Proteomics

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ORFans are hypothetical proteins lacking any significant sequence similarity with other proteins. Here, we highlighted by quantitative proteomics the TGAM_1934 ORFan from the hyperradioresistant Thermococcus gammatolerans archaeon as one of the most abundant hypothetical proteins. This protein has been selected as a priority target for structure determination on the basis of its abundance in three cellular conditions. Its solution structure has been determined using multidimensional heteronuclear NMR spectroscopy. TGAM_1934 displays an original fold, although sharing some similarities with the 3D structure of the bacterial ortholog of frataxin, CyaY, a protein conserved in bacteria and eukaryotes and involved in iron-sulfur cluster biogenesis. These results highlight the potential of structural proteomics in prioritizing ORFan targets for structure determination based on quantitative proteomics data. The proteomic data and structure coordinates have been deposited to the ProteomeXchange with identifier PXD000402 (http://proteomecentral.proteomexchange.org/dataset/PXD000402) and Protein Data Bank under the accession number 2mcf, respectively.

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Section: Resultsmentioning

confidence: 91%

“…TGAM_1934 was considered throughout this study as an ORFAN because until recently no related sequence was [36]. Remarkably, they were all isolated from hydrothermal vents and are hyperthermophilic and anaerobic species.…”

Section: Tgam_1934 Is Found In a Highly Conserved Thermococcus Genomimentioning

confidence: 99%

Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans

Yang

Fernandez²,

Lagorce

et al. 2015

Proteomics

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“…T. nautili qualifies as a new species and the characterization of its physiology and metabolism has been reported (2). …”

Section: Genome Announcementmentioning

confidence: 99%

Genome Sequence of a Hyperthermophilic Archaeon, Thermococcus nautili 30-1, That Produces Viral Vesicles

et al. 2014

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“…In the present work, we report the discovery and characterization of a new integrase from the hyperthermophilic archaeon Thermococcus nautili [40,41] capable of catalyzing both site-specific recombination and low sequence specificity recombination reactions mimicking homologous recombination. The wide distribution of this particular Y-recombinase among the Thermococcus genus provides a valid rationale for the observed genomic rearrangements in these Archaea.…”

Section: Introductionmentioning

confidence: 99%

Flipping chromosomes in deep-sea archaea

et al. 2017

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One of the major mechanisms driving the evolution of all organisms is genomic rearrangement. In hyperthermophilic Archaea of the order Thermococcales, large chromosomal inversions occur so frequently that even closely related genomes are difficult to align. Clearly not resulting from the native homologous recombination machinery, the causative agent of these inversions has remained elusive. We present a model in which genomic inversions are catalyzed by the integrase enzyme encoded by a family of mobile genetic elements. We characterized the integrase from Thermococcus nautili plasmid pTN3 and showed that besides canonical site-specific reactions, it catalyzes low sequence specificity recombination reactions with the same outcome as homologous recombination events on DNA segments as short as 104bp both in vitro and in vivo, in contrast to other known tyrosine recombinases. Through serial culturing, we showed that the integrase-mediated divergence of T. nautili strains occurs at an astonishing rate, with at least four large-scale genomic inversions appearing within 60 generations. Our results and the ubiquitous distribution of pTN3-like integrated elements suggest that a major mechanism of evolution of an entire order of Archaea results from the activity of a selfish mobile genetic element.

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Thermococcus nautili sp. nov., a hyperthermophilic archaeon isolated from a hydrothermal deep-sea vent

Cited by 27 publications

References 75 publications

Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans

Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans

Genome Sequence of a Hyperthermophilic Archaeon, Thermococcus nautili 30-1, That Produces Viral Vesicles

Flipping chromosomes in deep-sea archaea

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