Thermophiles as a Promising Source of Exopolysaccharides with Interesting Properties

Kambourova, Margarita; Radchenkova, Nadja; Tomova, Iva; Bojadjieva, Ivanka

doi:10.1007/978-3-319-13521-2_4

Cited by 16 publications

(6 citation statements)

References 86 publications

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“…EPSs produced by the thermophiles are reported to have good thermal stability and several biotechnological properties. They are capable of stabilizing other molecules, show good synergy with hydrocolloids, and have antimicrobial, and immunostimulant properties determining their possible future applications [ 10 ]. Bacterial EPSs have been considered promising antioxidants to develop effective functional foods with a longer shelf life [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

et al. 2022

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Extremophilic microorganisms often produce novel bioactive compounds to survive under harsh environmental conditions. Exopolysaccharides (EPSs), a constitutive part of bacterial biofilm, are functional biopolymers that act as a protecting sheath to the extremophilic bacteria and are of high industrial value. In this study, we elucidate a new EPS produced by thermophilic Bacillus haynesii CamB6 from a slightly acidic (pH 5.82) Campanario hot spring (56.4 °C) located in the Central Andean Mountains of Chile. Physicochemical properties of the EPS were characterized by different techniques: Scanning electron microscopy- energy dispersive X-ray spectroscopy (SEM-EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D and 2D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). The EPS demonstrated amorphous surface roughness composed of evenly distributed macromolecular lumps. GPC and HPLC analysis showed that the EPS is a low molecular weight heteropolymer composed of mannose (66%), glucose (20%), and galactose (14%). FTIR analysis demonstrated the polysaccharide nature (–OH groups, Acetyl groups, and pyranosic ring structure) and the presence of different glycosidic linkages among sugar residues, which was further confirmed by NMR spectroscopic analyses. Moreover, D-mannose α-(1→2) and α-(1→4) linkages prevail in the CamB6 EPS structure. TGA revealed the high thermal stability (240 °C) of the polysaccharide. The functional properties of the EPS were evaluated for food industry applications, specifically as an antioxidant and for its emulsification, water-holding (WHC), oil-holding (OHC), and flocculation capacities. The results suggest that the study EPS can be a useful additive for the food-processing industry.

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Section: Introductionmentioning

confidence: 99%

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

et al. 2022

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“…The results showed that LB malt was a more suitable medium than MB malt to obtain high cell densities, while MB malt was a better option for the production of EPSs. However, the overall EPSs productivity of R. marinus DSM 16675 was low (3- to 40-fold) in comparison to other thermophilic bacteria (Kambourova et al 2016) and needs further improvement. The monosaccharide composition of the EPSs differed, as the EPSs produced in MB malt also contained xylose, while this was not the case for the EPSs produced in cultures grown in LB malt .…”

Section: Resultsmentioning

confidence: 92%

“…The total production of EPSs by R. marinus DSM 16675 was low in comparison to thermophiles with good production of EPSs, shown to reach EPS concentrations in the range of 55–897 mg/L (Kambourova et al 2016). The reason might be due to harvesting the cells at the beginning of the stationary phase, since all good EPSs producers were reported to synthesize EPSs mainly during a prolonged stationary phase at low cell densities (Kambourova et al 2016).…”

Section: Resultsmentioning

confidence: 99%

Cultivation technology development of Rhodothermus marinus DSM 16675

et al. 2019

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This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LBmalt) resulted in higher cell density (OD620 = 6.6) than use of MBmalt (OD620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD620 = 20) in LBmalt and eightfold (OD620 = 14) in MBmalt. In both single and sequential batches, the production of carotenoids and EPSs using LBmalt was detected in the exponential phase and stationary phase, respectively, while in MBmalt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity (QE) of 0.67 (mg/L h) in MBmalt and the polymer contained xylose. In LB, QE was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.Electronic supplementary materialThe online version of this article (10.1007/s00792-019-01129-0) contains supplementary material, which is available to authorized users.

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“…The knowledge of thermophiles biodiversity could be very useful for the discovery of new molecules and/or more thermostable biocatalysts with thermophilic and thermostability properties superior to those currently known and in use [1,2]. Recent exploitations have clearly shown that thermophilic microorganisms are industrial providers of novel catalysts, and in some cases, these enzymes have been cloned and expressed in suitable mesophilic hosts [1,3].…”

Section: Introductionmentioning

confidence: 99%

“…The biggest part of thermophiles studied belongs to the genus Geobacillus, and several strains have been found in thermophilic and mesophilic environments [1,4]. The development of extremophiles from geothermal areas, as a resource for novel thermostable enzymes, has received attention even in Antarctica, where the presence of thermophiles has been indicated by the discovery of several geothermal areas [5].…”

Section: Introductionmentioning

confidence: 99%

Parageobacillus thermantarcticus, an Antarctic Cell Factory: From Crop Residue Valorization by Green Chemistry to Astrobiology Studies

et al. 2019

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Knowledge of Antarctic habitat biodiversity, both marine and terrestrial, has increased considerably in recent years, causing considerable development in the studies of life science related to Antarctica. In the Austral summer 1986–1987, a new thermophilic bacterium, Parageobacillus thermantarcticus strain M1 was isolated from geothermal soil of the crater of Mount Melbourne (74°22′ S, 164°40′ E) during the Italian Antarctic Expedition. In addition to the biotechnological potential due to the production of exopolysaccharides and thermostable enzymes, successful studies have demonstrated its use in the green chemistry for the transformation and valorization of residual biomass and its employment as a suitable microbial model for astrobiology studies. The recent acquisition of its genome sequence opens up new opportunities for the use of this versatile bacterium in still unexplored biotechnology sectors.

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Thermophiles as a Promising Source of Exopolysaccharides with Interesting Properties

Cited by 16 publications

References 86 publications

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

Cultivation technology development of Rhodothermus marinus DSM 16675

Parageobacillus thermantarcticus, an Antarctic Cell Factory: From Crop Residue Valorization by Green Chemistry to Astrobiology Studies

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