Thermophilic Enterococcus faecium QU 50 enabled open repeated batch fermentation for <scp>l</scp>-lactic acid production from mixed sugars without carbon catabolite repression

Tan, Jiaming; Abdel-Rahman, Mohamed Ali; Numaguchi, M.; Tashiro, Yukihiro; Zendo, Takeshi; Sakai, Kenji; Sonomoto, Kenji

doi:10.1039/c7ra03176a

Cited by 19 publications

(6 citation statements)

References 39 publications

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“…The repeated batch process involves repeated cycles of fermentation by reinoculating a part or all of the cells from one batch fermentation broth into the next batch fermentation medium [30]. In comparison with batch or fed batch culture, repeated batch operation has proved to have several advantages in increasing LA productivity besides saving the time and labor work [30]. BoM1-2 strain was firstly cultivated in repeated batch fermentation using 60 g L −1 of glucose for 10 runs at controlled pH 9.0.…”

Section: Discussionmentioning

confidence: 99%

High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration

Abdel-Rahman

Hassan

Azab

et al. 2019

BioMed Research International

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Optically pure lactic acid (LA) is an important chemical platform that has a wide range of industrial and biotechnological applications. Improved parameters for cost effective LA production are of great interest for industrial developments. In the present study, an alkaliphilic lactic acid bacterium, BoM 1-2, was selected among 369 newly obtained bacterial isolates. It was characterized using API 50 CHL kit and identified as Enterococcus hirae BoM 1-2 by 16S rRNA gene sequence analysis. Efficient polymer-grade L-lactic acid production was achieved at pH 9.0 and 40°C. In batch fermentation strategy using 20 g L−1 glucose, 19.6 g L−1 lactic acid was obtained with volumetric productivity of 2.18 g L−1 h−1. While using 100 g L−1 glucose, 96.0 g L−1 lactic acid was obtained with volumetric productivity of 1.07 g L−1 h−1. The highest lactic acid concentration of 180.6 g L−1 was achieved in multipulse fed batch strategy with volumetric productivity of 0.65 g L−1 h−1. To achieve higher productivity, repeated fermentation processes were applied using the two different strategies. In the first strategy, the lactic acid productivity was increased from 1.97 g L−1 h−1 to 4.48 g L−1 h−1 when the total of 10 repeated runs were carried out using 60 g L−1 glucose, but lactic acid productivity decreased to 2.95 g L−1 h−1 using 100 g L−1 glucose. In second strategy, repeated fermentation coupled with gradual increase in glucose concentration from 40 to 100 g L−1 was conducted for 24 runs. A dramatic increase in LA productivity up to 39.9 g L−1 h−1 (18-fold compared to first run) was achieved using 40 g L−1 glucose while volumetric productivity ranging between 24.8 and 29.9 g L−1 h−1 was achieved using 60–100 g L−1 glucose.

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Section: Discussionmentioning

confidence: 99%

High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration

Abdel-Rahman

Hassan

Azab

et al. 2019

BioMed Research International

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“…The results showed that a L-LA productivity of 17.55 g•(L•h) −1 was obtained [59]. The immobilized strain QU 50 could produce stable L-LA with yield of 1.01-1.02 g•g −1 in an open repeated batch fermentation using mixed sugars derived from lignocellulosic biomass [60]. The mycelium of Rhizopus oryzae NBRC 5384 was fixed in situ in sponge-like cubic particles, and CaCO 3 was added to control the pH of the culture solution.…”

Section: High-cell Density Cultivationmentioning

confidence: 97%

Lactic Acid Production by Fermentation of Biomass: Recent Achievements and Perspectives

Ren

Wang

et al. 2022

Sustainability

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Lactic acid is an important biochemical product. With the global pollution caused by plastics, especially marine plastics, the demand for lactic acid to produce polylactic acid has rapidly increased. However, the high costs of raw materials and fermentation–separation processes have severely limited lactic acid production. In this study, the research trend on lactic acid fermentation in recent years was analyzed by a bibliometric survey, and the latest progress in lactic acid fermentation using different biomass stocks and microorganisms is summarized. The effects of different fermentation modes and fermentation–separation coupling methods on lactic acid fermentation were analyzed. Finally, microbial strains for cooperative fermentation and polysaccharide utilization are discussed. It is meaningful to develop environmentally friendly, cost-effective in situ product removal technologies, use lactic acid as an intermediate to higher value-added products, and co-produce lactic acid and other products based on a biorefinery model.

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“…CCR is the central governing mechanism in LAB for the regulation of rare sugar uptake, which ensures cellular resource efficiency by selectively regulating the uptake and metabolism of favorable carbon sources in the presence of non-favorable carbon sources, leading to both the favorable and non-favorable carbon sources can be metabolized albeit in a different rate [ 59 ]. In brief, CCR in LAB is regulated by different elements participating in rare sugar transport and metabolism, including sugar phosphotransferase system (PTS), enzyme I (EI), phosphocarrier histidine protein (HPr), and catabolite control protein A (CcpA) acts as the trans -acting repressor [ 60 ].…”

Section: Marine Macroalgae: the Future Of Sustainable Bioenergy And B...mentioning

confidence: 99%

Sustainable circular biorefinery approach for novel building blocks and bioenergy production from algae using microbial fuel cell

et al. 2023

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The imminent need for transition to a circular biorefinery using microbial fuel cells (MFC), based on the valorization of renewable resources, will ameliorate the carbon footprint induced by industrialization. MFC catalyzed by bioelectrochemical process drew significant attention initially for its exceptional potential for integrated production of biochemicals and bioenergy. Nonetheless, the associated costly bioproduct production and slow microbial kinetics have constrained its commercialization. This review encompasses the potential and development of macroalgal biomass as a substrate in the MFC system for L-lactic acid (L-LA) and bioelectricity generation. Besides, an insight into the state-of-the-art technological advancement in the MFC system is also deliberated in detail. Investigations in recent years have shown that MFC developed with different anolyte enhances power density from several µW/m 2 up to 8160 mW/m 2 . Further, this review provides a plausible picture of macroalgal-based L-LA and bioelectricity circular biorefinery in the MFC system for future research directions.

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Thermophilic Enterococcus faecium QU 50 enabled open repeated batch fermentation for l-lactic acid production from mixed sugars without carbon catabolite repression

Abstract: Thermophilic lactic acid bacterium enabled homo-l-lactic acid fermentation from hexose/pentose without carbon catabolite repression, and open repeated production by immobilization.

Cited by 19 publications

References 39 publications

High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration

High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration

Lactic Acid Production by Fermentation of Biomass: Recent Achievements and Perspectives

Sustainable circular biorefinery approach for novel building blocks and bioenergy production from algae using microbial fuel cell

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