Thermosensitive nanogels based on dendritic polyglycerol and N-isopropylacrylamide for biomedical applications

Recent advances in topical delivery of proteins and peptides mediated by soft matter nanocarriers

“…Nevertheless, protein and peptide delivery via the nonparenteral route are highly appealing due to their non-invasive nature and needs to be further addressed in the future. For other applications and indications intensive research efforts are already conducted resulting in highly sophisticated soft matter nanocarriers like charge conversional polyionic micelles (Lee et al, 2009) or stimuli-responsive polymeric micelles which release the payload depending on the environmental pH (Koyamatsu et al, 2014), temperature (Cuggino et al, 2011) or ionic strength (Lu et al, 2014).…”

Design, Synthesis, Characterization and Toxicity Studies of Poly (N-Iso- Propylacrylamide-co-Lucifer Yellow) Particles for Drug Delivery Applications

“…Results suggest that doses above 0.5 mg/mL are required to cause adverse effects on the cells. It is also worth mentioning that the particle could penetrate the cell membrane of lung cancer cells A549 incubated at 37°C and were distributed in the perinuclear region [55].…”

“…The cytotoxicity and uptake of p(NIPAM) based nanoparticles with incorporated hyperbranched polyglycerol (HPG) was investigated by Cuggino et al [55]. The cytotoxic effect of p(NIPAM)-co-HPG nanoparticles on epithelial human lung cancer cell line A549, human hematopoietic cells U-937, and human epidermoid carcinoma cells A431.…”

Biocompatibility and characterization of polyglycerol-based thermoresponsive nanogels designed as novel drug-delivery systems and their intracellular localization in keratinocytes

“…The tNGs were synthesized and characterized in accordance to previously reported methods and protocols (Asadian-Birjand et al, 2012b;Cuggino et al, 2011a;Giulbudagian et al, 2014). Briefly, for the synthesis of pNIPAM-based nanogels (tNG_dPG_pNIPAM), pNIPAM (66 mg), acrylated dendritic polyglycerol (dPG-Ac 10% ) (33 mg), sodium dodecyl sulfate (SDS) (1.8 mg) and ammonium persulfate (APS) (2.8 mg) were dissolved in 5 mL of distilled water.…”

“…Interestingly, dPG can serve as a macromolecular reagent to fabricate cross-linked hydrophilic three-dimensional polymeric networks referred to as nanogels (Asadian-Birjand et al, 2012aKabanov & Vinogradov, 2009). Moreover, dPG-based nanogels that respond to external stimuli such as temperature may enable targeted and controlled drug transport (Bergueiro & Calderon, 2015;Cuggino et al, 2011a). In particular, thermoresponsive dPG-based nanogels (tNGs) are NGs fabricated of dPG cross-linked by thermoresponsive polymers such as poly(N-isopropylacrylamide) (pNIPAM) or poly(glycidyl methyl ether-co-ethyl glycidyl ether) (p(GME-co-EGE)), a linear polyglycerol derivate (tPG) (Cuggino et al, 2011b;Giulbudagian et al, 2014).…”