Thermostable ATP regeneration system using polyphosphate kinase from Thermosynechococcus elongatus BP-1 for d-amino acid dipeptide synthesis

Satô, Masaru; Masuda, Yusuke; Kirimura, Kohtaro; Kino, Kuniki

doi:10.1263/jbb.103.179

Cited by 58 publications

(31 citation statements)

References 23 publications

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“…In addition to Ec PPK1, there have been reports of using recombinant PPK1 enzymes from thermophilic organisms for ATP‐dependent applications at raised temperatures. Examples are the combination of a thermostable D ‐alanine‐ D ‐alanine ligase with PPK1 from the cyanobacterium Thermosynechococcus elongates 80 and the production of glycerol‐3‐phosphate with a glycerol kinase from Thermococcus kodakarensis and PPK1 from Thermus thermophilus. 81…”

Section: Emerging Enzymes and Phosphate Donors Used For In Vitro Amentioning

confidence: 99%

Emerging Enzymes for ATP Regeneration in Biocatalytic Processes

2015

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Adenosine-5'-triphosphate-dependent enzyme catalysed reactions are widespread in nature. Consequently, the enzymes involved have an intrinsic potential for use in syntheses of high value products. Although regeneration systems for ATP starting from adenosine-5'-diphosphate are available, certain limitations exist for both in vitro and in vivo applications requiring ATP regeneration from adenosine-5'-monophosphate, or adenosine. Following a short overview of the chemical and thermodynamic background, this Minireview focuses on emerging enzymes and methodologies for ATP regeneration. A large range of as yet unexploited reactions will be accessible with new, powerful, multistep ATP regeneration systems that use cheap phosphate donors and provide high longevity, compatibility, and robustness under process conditions. Their potential might go far beyond the direct use of ATP in enzymatic reactions; enzyme discovery, and engineering, as well as immobilisation strategies, will help to realise such systems.

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Section: Emerging Enzymes and Phosphate Donors Used For In Vitro Amentioning

confidence: 99%

Emerging Enzymes for ATP Regeneration in Biocatalytic Processes

2015

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“…PPK from Thermus thermophilus , which shows a 30% amino acid sequence homology to Ec PPK1, generated fructose 1,6-diphosphate for at least one week at 70°C [115]. Sato and colleges studied ATP-requiring D-amino acid dipeptide synthesis using PPK from Thermosynechococcus [116], but this enzyme was less thermostable than Thermus thermophilus PPK [115]. …”

Section: Possible Ppk Applications In Industrymentioning

confidence: 99%

Polyphosphate - an ancient energy source and active metabolic regulator

2011

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There are a several molecules on Earth that effectively store energy within their covalent bonds, and one of these energy-rich molecules is polyphosphate. In microbial cells, polyphosphate granules are synthesised for both energy and phosphate storage and are degraded to produce nucleotide triphosphate or phosphate. Energy released from these energetic carriers is used by the cell for production of all vital molecules such as amino acids, nucleobases, sugars and lipids. Polyphosphate chains directly regulate some processes in the cell and are used as phosphate donors in gene regulation. These two processes, energetic metabolism and regulation, are orchestrated by polyphosphate kinases. Polyphosphate kinases (PPKs) can currently be categorized into three groups (PPK1, PPK2 and PPK3) according their functionality; they can also be divided into three groups according their homology (EcPPK1, PaPPK2 and ScVTC). This review discusses historical information, similarities and differences, biochemical characteristics, roles in stress response regulation and possible applications in the biotechnology industry of these enzymes. At the end of the review, a hypothesis is discussed in view of synthetic biology applications that states polyphosphate and calcium-rich organelles have endosymbiotic origins from ancient protocells that metabolized polyphosphate.

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“…1999). Thermostable polyphosphate kinase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP‐1 was successfully employed in an ATP regeneration system that could be used at high temperatures for the effective production of d‐amino acid dipeptides (Sato et al. 2007).…”

Section: Cyanobacterial Bioactive Compoundsmentioning

confidence: 99%

Applications of cyanobacteria in biotechnology

Abed

Dobretsov

Sudesh

2009

Journal of Applied Microbiology

451

238

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Summary Cyanobacteria have gained a lot of attention in recent years because of their potential applications in biotechnology. We present an overview of the literature describing the uses of cyanobacteria in industry and services sectors and provide an outlook on the challenges and future prospects of the field of cyanobacterial biotechnology. Cyanobacteria have been identified as a rich source of biologically active compounds with antiviral, antibacterial, antifungal and anticancer activities. Several strains of cyanobacteria were found to accumulate polyhydroxyalkanoates, which can be used as a substitute for nonbiodegradable petrochemical‐based plastics. Recent studies showed that oil‐polluted sites are rich in cyanobacterial consortia capable of degrading oil components. Cyanobacteria within these consortia facilitated the degradation processes by providing the associated oil‐degrading bacteria with the necessary oxygen, organics and fixed nitrogen. Cyanobacterial hydrogen has been considered as a very promising source of alternative energy, and has now been made commercially available. In addition to these applications, cyanobacteria are also used in aquaculture, wastewater treatment, food, fertilizers, production of secondary metabolites including exopolysaccharides, vitamins, toxins, enzymes and pharmaceuticals. Future research should focus on isolating new cyanobacterial strains producing high value products and genetically modifying existing strains to ensure maximum production of the desired products. Metagenomic libraries should be constructed to discover new functional genes that are involved in the biosynthesis of biotechnological relevant compounds. Large‐scale industrial production of the cyanobacterial products requires optimization of incubation conditions and fermenter designs in order to increase productivity.

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Thermostable ATP regeneration system using polyphosphate kinase from Thermosynechococcus elongatus BP-1 for d-amino acid dipeptide synthesis

Cited by 58 publications

References 23 publications

Emerging Enzymes for ATP Regeneration in Biocatalytic Processes

Emerging Enzymes for ATP Regeneration in Biocatalytic Processes

Polyphosphate - an ancient energy source and active metabolic regulator

Applications of cyanobacteria in biotechnology

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