Thiamine induced resistance to Plasmopara viticola in grapevine and elicited host–defense responses, including HR like-cell death

Boubakri, Hatem; Wahab, Mohamed Ali; Chong, Julie; Bertsch, Christophe; Mliki, Ahmed; Soustre-Gacougnolle, Isabelle

doi:10.1016/j.plaphy.2012.05.016

Cited by 87 publications

(71 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…H 2 O 2 production is an important part of grapevine defense system (Boubakri et al, 2012) and is considered as a signal molecule to activate disease resistance (Pastor et al, 2013). No significant H 2 O 2 production was observed in response to bacterium or fungus inoculation ( Figure 4A ).…”

Section: Resultsmentioning

confidence: 99%

Burkholderia phytofirmans PsJN Confers Grapevine Resistance against Botrytis cinerea via a Direct Antimicrobial Effect Combined with a Better Resource Mobilization

Miotto-Vilanova¹,

Jacquard²,

Courteaux³

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

Plant innate immunity serves as a surveillance system by providing the first line of powerful weapons to fight against pathogen attacks. Beneficial microorganisms and Microbial-Associated Molecular Patterns might act as signals to trigger this immunity. Burkholderia phytofirmans PsJN, a highly efficient plant beneficial endophytic bacterium, promotes growth in a wide variety of plants including grapevine. Further, the bacterium induces plant resistance against abiotic and biotic stresses. However, no study has deciphered triggered-mechanisms during the tripartite interaction between grapevine, B. phytofirmans PsJN and Botrytis cinerea. Herein, we showed that in contrast with classical rhizobacteria, which are restricted in the root system and act through ISR, B. phytofirmans PsJN is able to migrate until aerial part and forms at leaves surface a biofilm around B. cinerea mycelium to restrict the pathogen. Nevertheless, considering the endophytic level of PsJN in leaves, the plant protection efficacy of B. phytofirmans PsJN could not be explained solely by its direct antifungal effect. Deeper investigations showed a callose deposition, H 2 O 2 production and primed expression of PR1, PR2, PR5, and JAZ only in bacterized-plantlets after pathogen challenge. The presence of PsJN modulated changes in leaf carbohydrate metabolism including gene expression, sugar levels, and chlorophyll fluorescence imaging after Botrytis challenge. Our findings indicated that protection induced by B. phytofirmans PsJN was multifaceted and relied on a direct antifungal effect, priming of defense mechanisms as well as the mobilization of carbon sources in grapevine leaf tissues.

show abstract

Section: Resultsmentioning

confidence: 99%

Burkholderia phytofirmans PsJN Confers Grapevine Resistance against Botrytis cinerea via a Direct Antimicrobial Effect Combined with a Better Resource Mobilization

Miotto-Vilanova¹,

Jacquard²,

Courteaux³

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…H 2 O 2 accumulation was detected as an early response induced by bacterial speck infection in tomato pre-treated by the biocontrol agent P. putida (Ahn et al 2011). In the cases of the SAR inducing agents, DL-β-aminobutyric acid (BABA) or thiamine, activation of H 2 O 2 production takes place prior to pathogen infection (Baysal et al 2005;Boubakri et al 2012). Whether the oxidative burst observed in the current study is correlative to or needed for biochar-IR could be addressed by the use of H 2 O 2 quenching agents in future research.…”

Section: Discussionmentioning

confidence: 99%

Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling

et al. 2015

View full text Add to dashboard Cite

Aims The goal of this study was to identify the induced resistance pathway mediated by biochar in the tomatoBotrytis cinerea pathosystem. Methods Tomato wild types and mutants modified in their salicylic acid (SA), ethylene (ET) or jasmonic acid (JA) metabolism were grown in a potting medium amended with biochar produced at 450ºC from greenhouse wastes, to identify the possible pathway(s) involved in biochar-mediated resistance to B. cinerea.Early cellular response of H 2 O 2 accumulation was biochemically tested, and the transcriptional changes of 12 defense-related genes upon B. cinerea challenge of detached leaflets were analyzed. Results Biochar amendment resulted in about 50 % reduction in B. cinerea disease severity in all tested genotypes with the exception of a JA deficient mutant, def1. Biochar amendment induced priming of early as well as late-acting defense responses particularly in the genes Pti5 (ET-related) and Pi2 (JA-related), which are known to be crucial in resistance against B. cinerea. Stronger and earlier H 2 O 2 accumulation subsequent to B. cinerea inoculation in all genotypes was observed as a result of biochar amendment, with the exception of the def1 mutation. Conclusion Biochar-mediated IR in the B. cinerea-tomato pathosystem involves the JA pathway.

show abstract

“…Application of thiamine in plants have been shown to activate plant defence responses in Arabidopsis (Ahn et al, 2007). Furthermore, thiamine treatment in soyabean also enhances resistance to charcoal rot disease (Monaim, 2011), rice against sheath blight disease (Bahuguna et al, 2012), and grapevine against a disease caused by Plasmapora viticola (Boubakri et al, 2012). In Arabidopsis thaliana, thiamine treatment activated pathogenesisrelated protein (PR-1) and phenylalanine lyase (PAL) (Ahn et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…In Arabidopsis thaliana, thiamine treatment activated pathogenesisrelated protein (PR-1) and phenylalanine lyase (PAL) (Ahn et al, 2007). Similarly, thiamine treatment in grapevine reduced downy mildew development compared to untreated control in a dose-dependent manner by inducing hydrogen peroxide generation, callose disposition and host resistance (HR) cell death (Boubakri et al, 2012). Therefore, thiamine is involved in priming, which is important to mitigate any crop-damaging diseases and stresses.…”

Section: Introductionmentioning

confidence: 99%

THIAMINE BIOSYNTHESIS GENE EXPRESSION ANALYSIS IN Elaeis guineensis DURING INTERACTIONS WITH Hendersonia toruloidea

Kamaruddin¹,

Seman²,

Yusof³

2017

JOPR

View full text Add to dashboard Cite

Figure 1 consists of two separate branches, which is the synthesis of thiazole and pyrimidine moieties. The condensation of pyrimidine and thiazole ring moieties will form thiamine monophosphate, which is then converted to the active form thiamine pyrophosphate (TPP). TPP is a cofactor of pyruvate dehydrogenase which participates in the oxidative decarboxylation of pyruvate to acetyl-CoA, synthesis of amino acid such as valine, leucine and isolecucine, 2-oxoglutarate dehydrogenase which is involved in Krebs cycle (Goyer, 2010;Goyer and Haynes, 2011; Tunc-Ozdemir et al., 2009). TPP is also a cofactor for transketolase in the oxidative pentose phosphate pathway, which plays a role in the production of reactive oxidative species (ROS). Downstream reactions of TPP-dependent of Krebs cycle is the oxidative phosphorylation pathway where electron is generated to synthesise ATP, which is important in energy production in all living organisms. Fundamentally, thiamine forms an indispensable role in proper functioning in plants. DOI: https://doi.org/10.21894/jopr.2017DOI: https://doi.org/10.21894/jopr. .2902 218

show abstract

Thiamine induced resistance to Plasmopara viticola in grapevine and elicited host–defense responses, including HR like-cell death

Cited by 87 publications

References 37 publications

Burkholderia phytofirmans PsJN Confers Grapevine Resistance against Botrytis cinerea via a Direct Antimicrobial Effect Combined with a Better Resource Mobilization

Burkholderia phytofirmans PsJN Confers Grapevine Resistance against Botrytis cinerea via a Direct Antimicrobial Effect Combined with a Better Resource Mobilization

Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling

THIAMINE BIOSYNTHESIS GENE EXPRESSION ANALYSIS IN Elaeis guineensis DURING INTERACTIONS WITH Hendersonia toruloidea

Contact Info

Product

Resources

About