Thidiazuron-induced adventitious shoot regeneration of sweetpotato (Ipomoea batatas)

Gosukonda, Ramana; Porobodessai, Ananta; Blay, Essie; Prakash, Channapatna S.; Peterson, Curt M.

doi:10.1007/bf02632239

Cited by 22 publications

(17 citation statements)

References 19 publications

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“…TDZ possess powerful cytokinin-like activity and is a good substitute for some other cytokinins such as zeatin riboside, benzyl aminopurin (BA) and kinetin in plant tissue culture systems (Mok et al 1982;Gosukonda et al 1995;Genkov and Iordanka 1995). The effect of TDZ such as other cytokinines on photosynthetic pigment contents was also reported.…”

Section: Introductionmentioning

confidence: 98%

The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica

Pourebad

Motafakkerazad

Kosari‐Nasab

et al. 2015

Plant Cell Tiss Organ Cult

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Lallemantia iberica (Lamiaceae) has pharmaceutical and nutritional importance owing to production of oily seeds and synthesis of commercially secondary metabolites. In the present study, total phenol and flavonoids content and quantitative changes in the shoot and callus pigments of L. iberica were investigated. Moreover, some growth parameters and volatile content of in vitro grown shoots were analyzed. Nodal segments and epicotyldons were used as the explants for shoot and callus formation, respectively. In the multiplication stage of shoot culture, MS medium supplemented with six concentrations of TDZ was employed. For callus induction, 1 mg/l of TDZ, NAA and a combination of NAA and BA were used. Extracted volatile products were analyzed by GC-mass spectrometry. According to the results, the concentration range of TDZ from 0.22 to 0.66 mg/l improved the flavonoids production and the highest content was reported to be in 2 mg/l TDZ (157.5 ± 25.9 mg QE/ g); also, the highest amount of total phenol (3.86 ± 1.02 mg PYRO/g) was observed in 0.66 mg/l TDZ. For induced calli, among the three tested types of PGRs, TDZ treatment was efficient for total phenol and flavonoids. In general, the higher content of flavonoids was obtained from L. iberica shoots while the callus of this plant was efficient for total phenol production. 55 components were identified using GC-MS analysis indicating a low amount of mono and sesquiterpenes and high quantity of alkan hydrocarbons.

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Section: Introductionmentioning

confidence: 98%

The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica

Pourebad

Motafakkerazad

Kosari‐Nasab

et al. 2015

Plant Cell Tiss Organ Cult

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“…Sweet potato regeneration using meristems as explants has been described for many varieties and many researchers in the world have managed to develop regeneration systems that work in their specific environments [10]. However, regeneration tends to be genotype specific; thus there is need to try and adapt established protocols and modify them if necessary, to suit local conditions and local varieties.…”

Section: Introductionmentioning

confidence: 99%

Effect of Bap, Naa and Ga3, Either Alone or in Combination, on Meristem Culture and Plantlet Establishment in Sweet Potato (Cv Brondal)

Masekesa¹,

Gasura²,

Matikiti³

et al. 2016

AJFAND

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In Zimbabwe, the average sweet potato yield (6 t/ha) is relatively low when compared to Asian counterparts (17 t/ha). These low crop yields have been blamed on weevil infestations and viral infections which account for 60-90% of sweet potato yield losses in Africa. Meristem tip culture, a Centre for Potato Improvement (CIP) initiated tissue culture technique, has been widely used to eradicate viruses from clonally propagated crops and has been noted to be one of the instrumental techniques that helped China to increase sweet potato yields. In an effort to adopt the meristem tip culture technique for the production of virus-free planting material of a local sweet potato (cv Brondal), a study was conducted to evaluate the effect of Benzylamino purine (BAP), 1-Naphthaleneacetic acid (NAA) and Gibberellic acid (GA3) (either alone or in combination) on cultured Brondal meristems. The different hormonal treatments were assessed on the following parameters: plantlet regenerative capacity, multiple plantlet production, shoot height, average leaf number per shoot and average node number per shoot, ten weeks after meristem culture. All treatments containing a combination of BAP (1 mg -L) and GA3 (at either 5 mg -L, 10 mg -L, or 20 mg -L) had a significantly (p<0.01) higher plantlet regenerative capacity of 33-66% when compared to other treatment combinations. Only treatments, 10 mg -L GA3 + 1 mg -L BAP and 20 mg -L GA3 + 1 mg -L BAP were capable of inducing multiple plantlet formation, producing an average of three plantlets/meristem and two plantlets/meristem respectively. Overall, treatment 10 mg -L GA3 + 1 mg -L BAP gave rise to significantly (p<0.01) taller shoots (20 mm) compared to the rest of the treatments used. For average leaf number per shoot, all GA3 treatments (5 mg -L, 10 mg -L, or 20 mg -L) supplemented with 1 mg -L BAP gave significantly (p<0.01) higher numbers of leaves (six leaves/shoot) than the rest of the treatments. Treatments 10 mg -L GA3 + 1 mg -L BAP and 20 mg -L GA3 + 1 mg -L BAP gave rise to the highest number of nodes per shoot, producing an average of three nodes per shoot. In sharp contrast to treatments containing a combination of BAP and GA3, all treatments containing a combination of BAP and NAA performed poorly in all parameters tested for plant regeneration of Brondal sweet potato variety. In conclusion, the best hormonal treatment for culturing Brondal meristems proved to be 10 mg -L GA3 + 1 mg -L BAP.

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“…A higher number of shoots developed from protuberances on a medium containing 5.0 μM TDZ compared to a medium containing 10.0 μM TDZ (Table 1). Gosukonda et al (1995) and Santa-Maria et al (2009) also reported adventitious shoot induction from leaf explants of sweetpotato on a stage II medium, where the explants were incubated for a few days on a medium containing auxins, and were subsequently transferred to a medium supplemented with cytokinins, which resulted in the development of 1-2 shoots per explant with a significant genotype and media interaction. In this study, we obtained relatively few-times more shoots per explant compared to earlier reports Santa-Maria et al, 2009).…”

Section: Production Of Protuberances From Leaf Explantsmentioning

confidence: 93%

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Kumar¹,

Ku²,

Agrawal³

et al. 2013

bta

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In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron (TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants. It appeared that the frequency of protuberance development depended on the concentration of TDZ. However, this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully established in a greenhouse.

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Thidiazuron-induced adventitious shoot regeneration of sweetpotato (Ipomoea batatas)

Cited by 22 publications

References 19 publications

The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica

The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica

Effect of Bap, Naa and Ga3, Either Alone or in Combination, on Meristem Culture and Plantlet Establishment in Sweet Potato (Cv Brondal)

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

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