Thidiazuron induced efficient in vitro organogenesis and regeneration of Scutellaria bornmuelleri: an important medicinal plant

Gharari, Zahra; Bagheri, Khadijeh; Sharafi, Ali; Danafar, Hossein

doi:10.1007/s11627-019-09965-7

Cited by 21 publications

(8 citation statements)

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“…Among the species of the genus Scutellaria , the following cultures have now been obtained and studied in vitro: Scutellaria baicalensis , Scutellaria lateriflora , Scutellaria barbata , Scutellaria columnae , Scutellaria alpine , Scutellaria brevibracteata and Scutellaria bornmuelleri [ 22 , 23 , 24 , 25 , 26 , 27 ]. The most well-known species of the genus Scutellaria — S. baicalensis —has been used in traditional Chinese medicine for a long time, and it is the most studied [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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We measured and studied the growth parameters and the qualitative and quantitative composition of the flavones of hairy roots of the Scutellaria genus: S. lateriflora, S. przewalskii and S. pycnoclada. Hairy roots were obtained using wild-type Agrobacterium rhizogenes A4 by co-cultivation of explants (cotyledons) in a suspension of Agrobacterium. The presence of the rol-genes was confirmed by PCR analysis. The hairy roots of the most studied plant from the Scutellaria genus, S. baicalensis, were obtained earlier and used as a reference sample. HPLC-MS showed the predominance of four main flavones (baicalin, baicalein, wogonin and wogonoside) in the methanol extracts of the studied hairy roots. In addition to the four main flavones, the other substances which are typical to the aerial part of plants were found in all the extracts: apigenin, apigetrin, scutellarin and chrysin-7-O-β-d-glucuronide. According to the total content of flavones, the hairy roots of the studied skullcaps form the following series: S. przewalskii (33 mg/g dry weight) > S. baicalensis (17.04 mg/g dry weight) > S. pycnoclada (12.9 mg/g dry weight) > S. lateriflora (4.57 mg/g dry weight). Therefore, the most promising producer of anti-coronavirus flavones is S. przewalskii.

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Section: Introductionmentioning

confidence: 99%

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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“…1C); on average, 8.33 ± 0.83 adventitious shoots were proliferated from each original shoot. TDZ is an efficient cytokinin for GGB proliferation in fern plant Cibotium barometz and adventitious shoot induction in seed plants, such as Erythrina variegate (Javed et al, 2019), Fraxinus nigra (Lee and Pijut, 2017), and Scutellaria bornmuelleri (Gharari et al, 2019). Nevertheless, the addition of TDZ, especially high concentrations (0.5 and 1.0 mg • L -1 ), easily resulted in the abnormal color of adventitious shoots in S. pulvinata (Fig.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants

Yu¹,

Cheng²,

Pu³

et al. 2021

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The resurrection plant Selaginella pulvinata (Hook. & Grev.) Maxim is used as an ornamental and medicinal plant. It is also a good candidate for exploring the desiccation tolerance of resurrection plants. However, there is not an efficient propagation method for S. pulvinata. In the present study, we evaluated the establishment of in vitro propagation of S. pulvinata using frond tips as explants. The original shoot induction, adventitious shoot proliferation and plantlet growth media, and substrate type of plantlet acclimatization were investigated. The highest induction rate of original shoots (61.77 ± 5.17%) was obtained on half-strength (1/2) MS medium supplemented with 0.1 mg·L−1 N6-benzylaminopurine (BAP). The 1/2 MS with 1.0 mg·L−1 BAP was the most effective medium for the adventitious shoot proliferation. The quarter-strength (1/4) MS containing 0.1% (w/v) active charcoal (AC) was optimum for plantlets proliferated from adventitious shoots and plantlet growth. Approximately 98 plantlets could be obtained from one single original shoot via one-time shoot proliferation cultivation and plantlet cultivation. The acclimated plants on a 5:1 (v/v) mixture of peat and perlite had the highest survival rate (92.13 ± 1.67%). The acclimated plants maintained excellent resurrection ability.

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“…In contrast, Zhang et al [ 17 ] reported that calli induced by 0.3 mg × dm −3 TDZ produced shoots directly, while those obtained with a higher TDZ concentration required a change of medium composition to initiate the process. In turn, Gharari et al [ 18 ] indicated that by increasing TDZ concentrations (0.5 mg × dm −3 ), the response rate of explants for shoot induction increased. Therefore, in connection with the aim of the research, it is necessary to indicate that potential genetic variability should be sought among the regenerants obtained on medium containing the combination of KIN + BAP (0.5 mg × dm −3 each).…”

Section: Resultsmentioning

confidence: 99%

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

Gawroński

Dyduch‐Siemińska

2022

Genes

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The in vitro culture technique can be used for micropropagation of medicinal plants as well as for creating genotypes with an improved profile of phytochemical compounds. For this purpose, somaclonal variability may be used for the induction of genetic diversity among regenerants. The paper presents a protocol for obtaining Scutellaria baicalensis regenerants by indirect organogenesis and the assessment of their genetic variability with the use of start codon-targeted markers. The most intense process of indirect shoot organogenesis was observed on Murashige and Skoog medium supplemented with kinetin and 6-Benzylaminopurine (0.5 mg × dm−3 each)—7.4 shoot per explant on average. The callogenesis process occurred on the medium supplemented with TDZ, while the medium supplemented with GA3 allowed for direct shoot organogenesis and was used for the micropropagation of regenerants. In the analysis of plantlets obtained by indirect organogenesis, 11 ScoT markers generated a total of 130 amplicons, 45 of which were polymorphic. This analysis showed genetic diversity of regenerants in relation to the donor plant as well as within them, with mean similarity among the analyzed genotypes at the level of 0.90. This study confirms that the use of in vitro cultures allows for the possibility to generate genetic variability in Scutellaria baicalensis, which can be effectively revealed with the use of the SCoT marker.

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Thidiazuron induced efficient in vitro organogenesis and regeneration of Scutellaria bornmuelleri: an important medicinal plant

Cited by 21 publications

References 20 publications

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants

Potential of In Vitro Culture of Scutellaria baicalensis in the Formation of Genetic Variation Confirmed by ScoT Markers

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