Thidiazuron Induced In Vitro Clonal Propagation of Lagerstroemia speciosa (L.) Pers.—An Important Avenue Tree

Ahmad, Naseem; Faisal, Mohammad; Ahmad, Anees; Alatar, Abdulrahman A.; Qahtan, Ahmed A.; Alok, Anshu

doi:10.3390/horticulturae8050359

Cited by 5 publications

(4 citation statements)

References 61 publications

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“…The supplementation with TDZ in the culture media efficiently activated the antioxidant system in the in vitro raised shoots, leading to maximum production of total phenolic content, total flavonoid content, antioxidant enzymes and lignans [ 55 ]. In a recent study, TDZ was shown to be effective in inducing in vitro clonal propagation of Lagerstroemia speciosa (L.) Pers., commonly known as ‘Pride of India’ or ‘Banaba’, belonging to the family Lythraceae, and is an important avenue tropical deciduous tree widely distributed in the Philippines, Malaysia, India, Vietnam, and China [ 56 ]. Furthermore, TDZ has induced efficient in vitro organogenesis and regeneration of Scutellaria bornmuelleri , a medicinal plant belonging to the Scutellaria genus of the Lamiaceae family, endemic in the East Azerbaijan province of Iran, used in traditional medicines to treat constipation, wound healing, and stress [ 57 ], and has increased shoot induction and proliferation rate of Tecoma stans L. (Bignoniaceae), commonly known as Ginger-Thomas, a plant endowed with antitumor, antioxidant, antimicrobial, antidiabetic and free radical scavenging properties [ 58 ].…”

Section: Thidiazuron (Tdz)mentioning

confidence: 99%

Thidiazuron: New Trends and Future Perspectives to Fight Xylella fastidiosa in Olive Trees

et al. 2022

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These days, most of our attention has been focused on the COVID-19 pandemic, and we have often neglected what is happening in the environment. For instance, the bacterium Xylella fastidiosa re-emerged as a plant pathogen of global importance in 2013 when it was first associated with an olive tree disease epidemic in Italy, called Olive Quick Decline Syndrome (OQDS), specifically caused by X. fastidiosa subspecies pauca ST53, which affects the Salento olive trees (Apulia, South-East Italy). This bacterium, transmitted by the insect Philaenus spumarius, is negatively reshaping the Salento landscape and has had a very high impact in the production of olives, leading to an increase of olive oil prices, thus new studies to curb this bacterium are urgently needed. Thidiazuron (TDZ), a diphenylurea (N-phenyl-1,2,3-thiadiazol-5-yl urea), has gained considerable attention in recent decades due to its efficient role in plant cell and tissue culture, being the most suitable growth regulator for rapid and effective plant production in vitro. Its biological activity against bacteria, fungi and biofilms has also been described, and the use of this low-cost compound to fight OQDS may be an intriguing idea.

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Section: Thidiazuron (Tdz)mentioning

confidence: 99%

Thidiazuron: New Trends and Future Perspectives to Fight Xylella fastidiosa in Olive Trees

et al. 2022

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“…However, many woody plants, including crapemyrtle, exhibit recalcitrance in the in-vitro organogenic process, necessitating resource-intensive experiments to nd the optimal balance of PGRs to promote organogenesis (Benson 2000;Bonga 2017;Long et al 2022;Tanimoto and Harada 1984;Thorpe and Harry 1990). Previous studies on micropropagation of L. speciosa, focused on the effects of various PGRs including thidiazuron (TDZ), 6-benzyladenine (6-BA), -naphthalene acetic acid (NAA), and N6-(3-hydroxybenzylamino purine) (meta-Topolin) (Ahmad et al 2022a;Ahmad et al 2022b; Lim-Ho and Lee 1985; Vijayan et al 2015), but few studies have examined callus-induced regeneration from leaf explants (Rahman et al 2010). A comprehensive investigation to optimize PGR combinations for callus induction, adventitious bud differentiation, shoot proliferation, and the subsequent rooting of regenerated plantlets is a crucial rst step in developing methodologies to apply modern breeding tools to L. speciosa and other taxa.…”

Section: Introductionmentioning

confidence: 99%

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

Wu,

Zhang,

Dixon

et al. 2023

Preprint

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Crapemyrtle (Lagerstroemia sp.) is the top-selling flowering tree in the U.S. However, threats from arthropod pests, including the recently emerged crapemyrtle bark scale (CMBS; Acanthococcus lagerstroemiae), severely jeopardize the aesthetic and production attributes of crapemyrtle. A tropical species, L. speciosa (L.) Pers. (“Queen's Crapemyrtle”) exhibits partial resistance to CMBS and other pests, but conventional breeding to incorporate the characteristics of L. speciosa into existing hybrids remains challenging. Recognizing the potential of tissue culture in facilitating molecular breeding, but also the possibility of undesirable somaclonal variations from in-vitro organogenesis, we utilized leaf explants of L. speciosa to develop a callus-induced regeneration protocol and assessed genetic fidelity of regenerated plantlets using inter-simple sequence repeat (ISSR) markers. Using woody plant medium (WPM) supplemented with 0.2 mg/L 2,4-D and 1.0 mg/L 6-BA achieved 97.9% callus induction. Shifting the growth regulators to 10.0 mg/L 6-BA and 0.5 mg/L NAA resulted in 32.4% of callus explants differentiating into adventitious buds. Finally, nodal segment proliferation (94.6%) and new shoot growth was maximized by using WPM supplemented with 1.0 mg/L 6-BA and 0.02 mg/L NAA. Explants rooted 100% using half-strength WPM supplemented with 0.2 mg/L IBA, and acclimatization survival was 98.3%. The ISSR primer analysis revealed 98.7% monomorphic markers, confirming the genetic integrity of the regenerated plantlets. We describe a reliable callus-induced regeneration system for L. speciosa, which will facilitate future molecular breeding and biotechnology to enhance cold hardiness, pest resistance, and other desired traits in this important genus.

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“…The exploitation of such new species as ornamental crops can be facilitated by the existence of a simple, productive and low-cost propagation protocol. Micropropagation using in vitro grown seedlings as starting material is a widespread method for the establishment of in vitro cultures, as it overcomes culture infection problems, reduces time needed for the establishment of in vitro cultures and usually leads to high proliferation rates [9,10,13,[20][21][22][23][24]. On the other hand, in vitro propagation initiated from seedlings can have a positive effect on the genetic diversity of the produced material compared to the use of other type of explants.…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, in addition to other cytokinins, the effect of thidiazuron (TDZ) (non-purine cytokinin), an artificially modified phenyl-urea (N-phenyl-N -1,2,3-thidiazol-5-yl-urea) was also tested for its effect on shoot proliferation. TDZ has been used in the in vitro propagation of various plant species at low concentrations [21,22,39]. The morphogenic response on TDZ media varies depending on the concentration, the type of explant, and genotype.…”

Section: Introductionmentioning

confidence: 99%

Studies on Seed Germination and Micropropagation of Ebenus sibthorpii, an Endemic Shrub of Greece with Potential Ornamental Use

Bertsouklis,

Vazaka-Vodena,

Bazanis

et al. 2023

Horticulturae

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The introduction of new ornamental species is a challenge for the floriculture industry. Ebenus sibthorpii is an endemic species of Greece, with a strong ornamental potential. To the best of our knowledge, there are no studies on the in vitro propagation of this species. Therefore, the current study aimed to determine the possibility of micropropagation of Ebenus sibthorpii as a first step for its introduction into the floriculture industry. A preliminary study on the germination ability of the species was also conducted on 1/2-strength Murashige and Skoog medium (MS/2), in the range of 5–35 °C. Seeds germinated at 46–64% indifferently of temperature between the range of 10–30 °C. In vitro cultures were successfully established (77–80% explant response) from single-node explants excised from seedlings, on MS medium either hormone-free (Hf) or supplemented with 6-benzyladenine (BA) at 0.5 and 1 mg L−1. The subsequent multiplication stage involved subcultures in MS medium either Hf or supplemented with various cytokinin types and concentrations, while the combination of 0.01 mg L−1 naphthaleneacetic acid (NAA) with BA was also tested. Τhe highest multiplication indices (3.6–4.6) were observed in media containing BA at 0.1–0.5 mg L−1, regardless of NAA presence, and in those with 0.5 mg L−1 ZEA or 1 mg L−1 TDZ. The highest number of shoots were formed in TDZ media, but almost half of them did not elongate. To address this, a two-fold culture was developed, where micro-shoots produced on TDZ media were transferred to Hf, MS or MS/2 medium to elongate. Thus, the highest multiplication index (16.4) was achieved when micro-shoots from 1 mg L−1 TDZ medium were transferred to MS medium. The present study could be the basis of further exploitation and conservation of E. sibthorpii.

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Thidiazuron Induced In Vitro Clonal Propagation of Lagerstroemia speciosa (L.) Pers.—An Important Avenue Tree

Cited by 5 publications

References 61 publications

Thidiazuron: New Trends and Future Perspectives to Fight Xylella fastidiosa in Olive Trees

Thidiazuron: New Trends and Future Perspectives to Fight Xylella fastidiosa in Olive Trees

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

Studies on Seed Germination and Micropropagation of Ebenus sibthorpii, an Endemic Shrub of Greece with Potential Ornamental Use

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