Thidiazuron Induces High Frequency Direct Somatic Embryogenesis Growth from Cotyledon Culture of Eurycoma longifolia

Dalila, Zawawi Dhiya; Hafsah, Ja’afar; Zainuddin, Rokiah; Rodziah, Kari; Madihah, Mohd Noor

doi:10.17576/jsm-2015-4407-01

Cited by 4 publications

(1 citation statement)

References 34 publications

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“…Furthermore, EC were used to induce SEs on liquid media containing 1–2.5 mg/L 2,4-D, 2.0 mg/L BAP, and kinetin [ 73 ]. Additionally, Dalila et al [ 74 ] found that the highest percentage of SE was produced using modified MS containing 0.1 mg/L zeatin, 0.2 mg/L IBA, and 0.12 mg/L TDZ. Mohd et al [ 75 ] also utilized the mentioned liquid media and varied the immersion frequencies in RITA ® bioreactors.…”

Section: Tissue Culture Techniques For Mass Propagation In ...mentioning

confidence: 99%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, various techniques have been used to mass propagate and improve the yield of these bioactive compounds in tissue cultures. These techniques include the optimization of media conditions and application of various types and combinations of plant growth regulators (PGRs). In addition, some elicitation techniques have been used to improve the synthesis of these bioactive compounds. However, in comparison with other herbal species with similar economic importance, many techniques have not been applied to E. longifolia. Adopting the most recent methodologies would ensure efficiency and sustainability in the in vitro production of bioactive compounds in E. longifolia. Therefore, in this review, we present an up-to-date record on the success stories in the tissue culture techniques and synthesis of bioactive compounds. In addition, we attempted to identify some of the missing links on the road to the effective and sustainable biotechnological utilization of this super important biological resource.

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Section: Tissue Culture Techniques For Mass Propagation In ...mentioning

confidence: 99%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

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The effect of 2,4-D, BA and Thidiazuron on somatic embryo induction of liberica coffee of Tungkal Composite from Jambi

et al. 2023

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The Tungkal Composite of liberica coffee rejuvenation program at Jambi Province is urgently needed due to the latest status of the plants are old and showing diseased attacked. To support the rejuvenation, the provision of seedlings could be done by tissue culture approach through somatic embryogenesis. This research aimed to study the effect of 2,4-D, BA and TDZ on callus formation and embryo induction from liberica coffee leaf explants. Explants used were leaf sections with or without veins. Explants were grown on Murashige Skoog (MS) basal medium with 2,4-D + BA and 2,4-D + TDZ. A completely randomized design was employed in the trial, and each experimental unit was replicated three times. Results showed that callus could be formed on both types of explants, but leaf explants with veins could produce callus faster. The use of BA in medium could induce callus formation faster than TDZ. The higher the concentration of 2,4-D, BA and TDZ, the slower callus initiation. The addition of TDZ in the medium resulted in mostly callus, while callus from medium with BA showed a friable structure. Globular somatic embryos were formed in all treatments with varying amounts. TDZ can induce more globular somatic embryo than BA.

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In Vitro Somatic Embryos Multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®

Mohd¹,

Hafsah²,

Zawawi³

et al. 2017

JSM

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Thidiazuron Induces High Frequency Direct Somatic Embryogenesis Growth from Cotyledon Culture of Eurycoma longifolia

Abstract: Eurycoma longifolia is one of the well-known herbal plants in Southeast Asian region due to its remarkable properties, especially the root part that can be used as aphrodisiac, anti-cancer, anti-malaria and anti-ulcer

Cited by 4 publications

References 34 publications

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

The effect of 2,4-D, BA and Thidiazuron on somatic embryo induction of liberica coffee of Tungkal Composite from Jambi

In Vitro Somatic Embryos Multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®

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